8gex - Revision history

OCA at 08:23, 14 August 2024

2024-08-14T08:23:14Z

← Older revision		Revision as of 08:23, 14 August 2024
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	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients such as iron. Pathogens scavenge iron using siderophores, ~~which~~ is counteracted by ~~the~~ host ~~using~~ lipocalin-2, ~~a protein that~~ sequesters iron-laden ~~siderophores, including~~ enterobactin. Although ~~the host and pathogens compete for~~ iron in the presence of gut ~~commensal~~ bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron in the inflamed gut by utilizing siderophores produced by other bacteria including Salmonella, via a secreted siderophore-binding lipoprotein ~~termed~~ XusB. Notably, XusB-bound ~~siderophores are~~ less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella , allowing the pathogen to evade nutritional immunity. As the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the interactions ~~between pathogen~~ and ~~host~~ nutritional immunity.		During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients, such as iron. Pathogens scavenge iron using siderophores, including enterobactin; however, this strategy is counteracted by host protein lipocalin-2, which sequesters iron-laden enterobactin. Although this iron competition occurs in the presence of gut bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron and sustains its resilience in the inflamed gut by utilizing siderophores produced by other bacteria, including Salmonella, via a secreted siderophore-binding lipoprotein XusB. Notably, XusB-bound enterobactin is less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella, allowing the pathogen to evade nutritional immunity. Because the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the host-pathogen interactions and nutritional immunity.

	Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.,Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, Torres TP~~, David HE~~, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W ~~bioRxiv [Preprint]~~. 2023 ~~Jun 26~~:~~2023.06~~.~~25.546471~~. doi: , 10.~~1101~~/2023.06.25.~~546471~~. PMID:~~37425782~~<ref>PMID:~~37425782~~</ref>		Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.,Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, David HE, Torres TP, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Folta-Stogniew E, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W Cell Host Microbe. 2023 Oct 11;31(10):1639-1654.e10. doi: , 10.1016/j.chom.2023.08.018. Epub 2023 Sep 29. PMID:37776864<ref>PMID:37776864</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 10:49, 22 May 2024

2024-05-22T10:49:54Z

← Older revision		Revision as of 10:49, 22 May 2024
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	During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients such as iron. Pathogens scavenge iron using siderophores, which is counteracted by the host using lipocalin-2, a protein that sequesters iron-laden siderophores, including enterobactin. Although the host and pathogens compete for iron in the presence of gut commensal bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron in the inflamed gut by utilizing siderophores produced by other bacteria including Salmonella, via a secreted siderophore-binding lipoprotein termed XusB. Notably, XusB-bound siderophores are less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella , allowing the pathogen to evade nutritional immunity. As the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the interactions between pathogen and host nutritional immunity.		During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients such as iron. Pathogens scavenge iron using siderophores, which is counteracted by the host using lipocalin-2, a protein that sequesters iron-laden siderophores, including enterobactin. Although the host and pathogens compete for iron in the presence of gut commensal bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron in the inflamed gut by utilizing siderophores produced by other bacteria including Salmonella, via a secreted siderophore-binding lipoprotein termed XusB. Notably, XusB-bound siderophores are less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella , allowing the pathogen to evade nutritional immunity. As the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the interactions between pathogen and host nutritional immunity.

	Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.,Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, Torres TP, David HE, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W bioRxiv. 2023 Jun 26:2023.06.25.546471. doi: 10.1101/2023.06.25.546471~~. Preprint~~. PMID:37425782<ref>PMID:37425782</ref>		Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.,Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, Torres TP, David HE, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W bioRxiv [Preprint]. 2023 Jun 26:2023.06.25.546471. doi: , 10.1101/2023.06.25.546471. PMID:37425782<ref>PMID:37425782</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 10:38, 2 August 2023

2023-08-02T10:38:04Z

← Older revision		Revision as of 10:38, 2 August 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8gex is ~~ON HOLD until Paper~~ Publication		==Crystal structure of the ferric enterobactin transporter (XusB) from Bacteroides thetaiotaomicron==
			<StructureSection load='8gex' size='340' side='right'caption='[[8gex]], [[Resolution\|resolution]] 2.55Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8gex]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GEX FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gex OCA], [https://pdbe.org/8gex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gex RCSB], [https://www.ebi.ac.uk/pdbsum/8gex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gex ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A0P0ENH6_BACT4 A0A0P0ENH6_BACT4]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			During intestinal inflammation, host nutritional immunity starves microbes of essential micronutrients such as iron. Pathogens scavenge iron using siderophores, which is counteracted by the host using lipocalin-2, a protein that sequesters iron-laden siderophores, including enterobactin. Although the host and pathogens compete for iron in the presence of gut commensal bacteria, the roles of commensals in nutritional immunity involving iron remain unexplored. Here, we report that the gut commensal Bacteroides thetaiotaomicron acquires iron in the inflamed gut by utilizing siderophores produced by other bacteria including Salmonella, via a secreted siderophore-binding lipoprotein termed XusB. Notably, XusB-bound siderophores are less accessible to host sequestration by lipocalin-2 but can be "re-acquired" by Salmonella , allowing the pathogen to evade nutritional immunity. As the host and pathogen have been the focus of studies of nutritional immunity, this work adds commensal iron metabolism as a previously unrecognized mechanism modulating the interactions between pathogen and host nutritional immunity.

	~~Authors:~~ Perera, ~~Y.R.~~, Chazin, W.J.		Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection.,Spiga L, Fansler RT, Perera YR, Shealy NG, Munneke MJ, Torres TP, David HE, Lemoff A, Ran X, Richardson KL, Pudlo N, Martens EC, Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W bioRxiv. 2023 Jun 26:2023.06.25.546471. doi: 10.1101/2023.06.25.546471. Preprint. PMID:37425782<ref>PMID:37425782</ref>

	~~Description: Crystal structure~~ of the ~~ferric enterobactin transporter (XusB) from~~ Bacteroides thetaiotaomicron		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: Chazin~~, W.J~~]]		<div class="pdbe-citations 8gex" style="background-color:#fffaf0;"></div>
	[[Category: Perera~~, Y.R~~]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Bacteroides thetaiotaomicron]]
			[[Category: Large Structures]]
			[[Category: Chazin WJ]]
			[[Category: Perera YR]]

OCA at 04:56, 7 April 2023

2023-04-07T04:56:35Z

← Older revision		Revision as of 04:56, 7 April 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8gex is ON HOLD		The entry 8gex is ON HOLD until Paper Publication

	Authors: Perera, Y.R., Chazin, W.J.		Authors: Perera, Y.R., Chazin, W.J.

OCA: Protected "8gex" [edit=sysop:move=sysop]

2023-03-15T07:51:03Z

Protected "8gex" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:51, 15 March 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8gex is ON HOLD Authors: Perera, Y.R., Chazin, W.J. Description: Crystal structure of the ferric enterobactin transporter (XusB) from Bacteroides t...

2023-03-15T07:50:59Z

New page: '''Unreleased structure''' The entry 8gex is ON HOLD Authors: Perera, Y.R., Chazin, W.J. Description: Crystal structure of the ferric enterobactin transporter (XusB) from Bacteroides t...

New page

'''Unreleased structure'''

The entry 8gex is ON HOLD

Authors: Perera, Y.R., Chazin, W.J.

Description: Crystal structure of the ferric enterobactin transporter (XusB) from Bacteroides thetaiotaomicron
[[Category: Unreleased Structures]]
[[Category: Chazin, W.J]]
[[Category: Perera, Y.R]]