8gpu - Revision history

OCA at 09:40, 17 October 2024

2024-10-17T09:40:02Z

← Older revision		Revision as of 09:40, 17 October 2024
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gpu OCA], [https://pdbe.org/8gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gpu RCSB], [https://www.ebi.ac.uk/pdbsum/8gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gpu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gpu OCA], [https://pdbe.org/8gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gpu RCSB], [https://www.ebi.ac.uk/pdbsum/8gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gpu ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	~~[https://www.uniprot.org/uniprot/Q89292_9FLAV Q89292_9FLAV]~~
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	The yellow fever virus (YFV) is a life-threatening human pathogen. Owing to the lack of available therapeutics, non-vaccinated individuals are at risk. Here, we isolated eight human monoclonal antibodies that neutralize YFV infection. Five recognized overlapping epitopes and exhibited potent neutralizing activity. Two (YD6 and YD73) were ultra-potent and conferred complete protection against the lethal challenge of YFV as both prophylactics and therapeutics in a mouse model. Crystal structures revealed that YD6 engaged the YFV envelope protein in both pre- and post-fusion states, suggesting viral inhibition by a "double-lock" mechanism. The recognition determinants for YD6 and YD73 are clustered at the premembrane (prM)-binding site. Notably, antibodies targeting this site were present in minute traces in YFV-infected individuals but contributed significantly to neutralization, suggesting a vulnerable supersite of YFV. We provide two promising candidates for immunotherapy against YFV, and the supersite represents an ideal target for epitope-based vaccine design.		The yellow fever virus (YFV) is a life-threatening human pathogen. Owing to the lack of available therapeutics, non-vaccinated individuals are at risk. Here, we isolated eight human monoclonal antibodies that neutralize YFV infection. Five recognized overlapping epitopes and exhibited potent neutralizing activity. Two (YD6 and YD73) were ultra-potent and conferred complete protection against the lethal challenge of YFV as both prophylactics and therapeutics in a mouse model. Crystal structures revealed that YD6 engaged the YFV envelope protein in both pre- and post-fusion states, suggesting viral inhibition by a "double-lock" mechanism. The recognition determinants for YD6 and YD73 are clustered at the premembrane (prM)-binding site. Notably, antibodies targeting this site were present in minute traces in YFV-infected individuals but contributed significantly to neutralization, suggesting a vulnerable supersite of YFV. We provide two promising candidates for immunotherapy against YFV, and the supersite represents an ideal target for epitope-based vaccine design.

	A neutralizing-protective supersite of human monoclonal antibodies for yellow fever virus.,Li Y, Chen Z, Wu L, Dai L, Qi J, Chai Y, Li S, Wang Q, Tong Z, Ma S, Duan X, Ren S, Song R, Liang M, Liu W, Yan J, Gao GF Innovation (Camb). 2022 Sep 15;3(6):100323. doi: 10.1016/j.xinn.2022.100323., eCollection 2022 Nov 8. PMID:36199277<ref>PMID:36199277</ref>		A neutralizing-protective supersite of human monoclonal antibodies for yellow fever virus.,Li Y, Chen Z, Wu L, Dai L, Qi J, Chai Y, Li S, Wang Q, Tong Z, Ma S, Duan X, Ren S, Song R, Liang M, Liu W, Yan J, Gao GF Innovation (Camb). 2022 Sep 15;3(6):100323. doi: 10.1016/j.xinn.2022.100323. , eCollection 2022 Nov 8. PMID:36199277<ref>PMID:36199277</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 18:13, 29 November 2023

2023-11-29T18:13:30Z

← Older revision		Revision as of 18:13, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[8gpu]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[8gpu]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GPU FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gpu OCA], [https://pdbe.org/8gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gpu RCSB], [https://www.ebi.ac.uk/pdbsum/8gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gpu ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.79Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gpu OCA], [https://pdbe.org/8gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gpu RCSB], [https://www.ebi.ac.uk/pdbsum/8gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gpu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:27, 3 November 2022

2022-11-03T07:27:02Z

@@ Line 1: / Line 1: @@
-The entry 8gpu is ON HOLD
+==YFV_E_YD6Fab_prefusion==
-Authors:
+A neutralizing-protective supersite of human monoclonal antibodies for yellow fever virus.,Li Y, Chen Z, Wu L, Dai L, Qi J, Chai Y, Li S, Wang Q, Tong Z, Ma S, Duan X, Ren S, Song R, Liang M, Liu W, Yan J, Gao GF Innovation (Camb). 2022 Sep 15;3(6):100323. doi: 10.1016/j.xinn.2022.100323., eCollection 2022 Nov 8. PMID:36199277<ref>PMID:36199277</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA: Protected "8gpu" [edit=sysop:move=sysop]

2022-09-07T15:39:39Z

Protected "8gpu" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:39, 7 September 2022
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8gpu is ON HOLD Authors: Description: Category: Unreleased Structures

2022-09-07T15:39:38Z

New page: '''Unreleased structure''' The entry 8gpu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8gpu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]