8ify - Revision history

OCA at 12:16, 23 October 2024

2024-10-23T12:16:36Z

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 == Function ==
 [https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref>   mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]  Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
 == References ==
 <references/>

OCA at 09:12, 30 August 2023

2023-08-30T09:12:09Z

← Older revision		Revision as of 09:12, 30 August 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8ify is ~~ON HOLD~~ ~~until Paper Publication~~		==Cryo-EM structure of SARS-CoV-2 Omicron BA.4/5 spike protein in complex with white-tailed deer ACE2==
			<StructureSection load='8ify' size='340' side='right'caption='[[8ify]], [[Resolution\|resolution]] 2.55Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[8ify]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Odocoileus_virginianus Odocoileus virginianus] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8IFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8IFY FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.55Å</td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ify FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ify OCA], [https://pdbe.org/8ify PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ify RCSB], [https://www.ebi.ac.uk/pdbsum/8ify PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ify ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Odocoileus virginianus]]
			[[Category: Severe acute respiratory syndrome coronavirus 2]]
			[[Category: Han P]]
			[[Category: Meng YM]]
			[[Category: Qi JX]]

OCA at 04:32, 23 August 2023

2023-08-23T04:32:27Z

← Older revision		Revision as of 04:32, 23 August 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8ify is ON HOLD		The entry 8ify is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "8ify" [edit=sysop:move=sysop]

2023-03-02T04:40:35Z

Protected "8ify" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:40, 2 March 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8ify is ON HOLD Authors: Description: Category: Unreleased Structures

2023-03-02T04:40:33Z

New page: '''Unreleased structure''' The entry 8ify is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 8ify is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]