OCA at 08:29, 16 August 2023

2023-08-16T08:29:51Z

← Older revision		Revision as of 08:29, 16 August 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8phy is ~~ON HOLD until Paper~~ Publication		==crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2 (2)==
			<StructureSection load='8phy' size='340' side='right'caption='[[8phy]], [[Resolution\|resolution]] 1.06Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8phy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Blumeria_graminis_f._sp._tritici Blumeria graminis f. sp. tritici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PHY FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.06Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8phy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8phy OCA], [https://pdbe.org/8phy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8phy RCSB], [https://www.ebi.ac.uk/pdbsum/8phy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8phy ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A1L5JEG4_BLUGR A0A1L5JEG4_BLUGR]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			In plants, host-pathogen coevolution often manifests in reciprocal, adaptive genetic changes through variations in host nucleotide-binding leucine-rich repeat immune receptors (NLRs) and virulence-promoting pathogen effectors. In grass powdery mildew (PM) fungi, an extreme expansion of a RNase-like effector family, termed RALPH, dominates the effector repertoire, with some members recognized as avirulence (AVR) effectors by cereal NLR receptors. We report the structures of the sequence-unrelated barley PM effectors AVR(A6), AVR(A7), and allelic AVR(A10)/AVR(A22) variants, which are detected by highly sequence-related barley NLRs MLA6, MLA7, MLA10, and MLA22 and of wheat PM AVR(PM2) detected by the unrelated wheat NLR PM2. The AVR effectors adopt a common scaffold, which is shared with the RNase T1/F1 family. We found striking variations in the number, position, and length of individual structural elements between RALPH AVRs, which is associated with a differentiation of RALPH effector subfamilies. We show that all RALPH AVRs tested have lost nuclease and synthetase activities of the RNase T1/F1 family and lack significant binding to RNA, implying that their virulence activities are associated with neo-functionalization events. Structure-guided mutagenesis identified six AVR(A6) residues that are sufficient to turn a sequence-diverged member of the same RALPH subfamily into an effector specifically detected by MLA6. Similar structure-guided information for AVR(A10) and AVR(A22) indicates that MLA receptors detect largely distinct effector surface patches. Thus, coupling of sequence and structural polymorphisms within the RALPH scaffold of PMs facilitated escape from NLR recognition and potential acquisition of diverse virulence functions.

	~~Authors:~~ Cao, Y., Gebauer, J~~.M.~~, Baumann, U., ~~Chai, J~~.J.		Structural polymorphisms within a common powdery mildew effector scaffold as a driver of coevolution with cereal immune receptors.,Cao Y, Kummel F, Logemann E, Gebauer JM, Lawson AW, Yu D, Uthoff M, Keller B, Jirschitzka J, Baumann U, Tsuda K, Chai J, Schulze-Lefert P Proc Natl Acad Sci U S A. 2023 Aug 8;120(32):e2307604120. doi: , 10.1073/pnas.2307604120. Epub 2023 Jul 31. PMID:37523523<ref>PMID:37523523</ref>

	~~Description~~: ~~crystal structure of powdery mildews~~ Blumeria graminis f. sp. tritici ~~AVRPM2 (2)~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Cao, Y~~]]		<div class="pdbe-citations 8phy" style="background-color:#fffaf0;"></div>
	[[Category: ~~Baumann, U~~]]		== References ==
	[[Category: Chai~~, J.J~~]]		<references/>
	[[Category: Gebauer~~, J.M~~]]		__TOC__
			</StructureSection>
			[[Category: Blumeria graminis f. sp. tritici]]
			[[Category: Large Structures]]
			[[Category: Baumann U]]
			[[Category: Cao Y]]
			[[Category: Chai JJ]]
			[[Category: Gebauer JM]]

OCA at 10:53, 26 July 2023

2023-07-26T10:53:45Z

← Older revision		Revision as of 10:53, 26 July 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8phy is ON HOLD		The entry 8phy is ON HOLD until Paper Publication

	Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J.		Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J.

OCA: Protected "8phy" [edit=sysop:move=sysop]

2023-06-28T19:53:21Z

Protected "8phy" [edit=sysop:move=sysop]

← Older revision	Revision as of 19:53, 28 June 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8phy is ON HOLD Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J. Description: crystal structure of powdery mildews Blumeria graminis f. sp....

2023-06-28T19:53:20Z

New page: '''Unreleased structure''' The entry 8phy is ON HOLD Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J. Description: crystal structure of powdery mildews Blumeria graminis f. sp....

New page

'''Unreleased structure'''

The entry 8phy is ON HOLD

Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J.

Description: crystal structure of powdery mildews Blumeria graminis f. sp. tritici AVRPM2 (2)
[[Category: Unreleased Structures]]
[[Category: Cao, Y]]
[[Category: Baumann, U]]
[[Category: Chai, J.J]]
[[Category: Gebauer, J.M]]

8phy - Revision history

OCA at 08:29, 16 August 2023

OCA at 10:53, 26 July 2023

OCA: Protected "8phy" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8phy is ON HOLD Authors: Cao, Y., Gebauer, J.M., Baumann, U., Chai, J.J. Description: crystal structure of powdery mildews Blumeria graminis f. sp....