OCA at 12:08, 8 November 2023

2023-11-08T12:08:08Z

← Older revision		Revision as of 12:08, 8 November 2023
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	~~'''Unreleased structure'''~~

	~~The entry~~ 8tkf is ~~ON HOLD~~		==Human Type 3 IP3 Receptor - Activated State (+IP3/ATP/JD Ca2+)==
			<StructureSection load='8tkf' size='340' side='right'caption='[[8tkf]], [[Resolution\|resolution]] 3.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8tkf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TKF FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=I3P:D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE'>I3P</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene>, <scene name='pdbligand=PT5:(1S)-2-{[(R)-HYDROXY{[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIHYDROXY-4,5-BIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}-1-[(OCTADECANOYLOXY)METHYL]ETHYL+(8E,11E)-ICOSA-5,8,11,14-TETRAENOATE'>PT5</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tkf OCA], [https://pdbe.org/8tkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tkf RCSB], [https://www.ebi.ac.uk/pdbsum/8tkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tkf ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ITPR3_HUMAN ITPR3_HUMAN] Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are endoplasmic reticulum Ca(2+) channels whose biphasic dependence on cytosolic Ca(2+) gives rise to Ca(2+) oscillations that regulate fertilization, cell division and cell death. Despite the critical roles of IP(3)R-mediated Ca(2+) responses, the structural underpinnings of the biphasic Ca(2+) dependence that underlies Ca(2+) oscillations are incompletely understood. Here, we collect cryo-EM images of an IP(3)R with Ca(2+) concentrations spanning five orders of magnitude. Unbiased image analysis reveals that Ca(2+) binding does not explicitly induce conformational changes but rather biases a complex conformational landscape consisting of resting, preactivated, activated, and inhibited states. Using particle counts as a proxy for relative conformational free energy, we demonstrate that Ca(2+) binding at a high-affinity site allows IP(3)Rs to activate by escaping a low-energy resting state through an ensemble of preactivated states. At high Ca(2+) concentrations, IP(3)Rs preferentially enter an inhibited state stabilized by a second, low-affinity Ca(2+) binding site. Together, these studies provide a mechanistic basis for the biphasic Ca(2+)-dependence of IP(3)R channel activity.

	~~Authors:~~ Paknejad, N., Sapuru, V., Hite~~, R~~.K.		Structural titration reveals Ca(2+)-dependent conformational landscape of the IP(3) receptor.,Paknejad N, Sapuru V, Hite RK Nat Commun. 2023 Oct 28;14(1):6897. doi: 10.1038/s41467-023-42707-3. PMID:37898605<ref>PMID:37898605</ref>

	~~Description~~: ~~Human Type 3 IP3 Receptor -Activated State (+IP3~~/~~ATP~~/~~JD Ca2+)~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Paknejad, N~~]]		<div class="pdbe-citations 8tkf" style="background-color:#fffaf0;"></div>
	[[Category: ~~Hite, R.K~~]]		== References ==
	[[Category: Sapuru, V]]		<references/>
			__TOC__
			</StructureSection>
			[[Category: Homo sapiens]]
			[[Category: Large Structures]]
			[[Category: Hite RK]]
			[[Category: Paknejad N]]
			[[Category: Sapuru V]]

OCA at 10:48, 1 November 2023

2023-11-01T10:48:58Z

← Older revision		Revision as of 10:48, 1 November 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8tkf is ON HOLD ~~until sometime in the future~~		The entry 8tkf is ON HOLD

	Authors: Paknejad, N., Sapuru, V., Hite, R.K.		Authors: Paknejad, N., Sapuru, V., Hite, R.K.

OCA: Protected "8tkf" [edit=sysop:move=sysop]

2023-08-02T09:25:19Z

Protected "8tkf" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:25, 2 August 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8tkf is ON HOLD until sometime in the future Authors: Paknejad, N., Sapuru, V., Hite, R.K. Description: Human Type 3 IP3 Receptor -Activated State ...

2023-08-02T09:25:18Z

New page: '''Unreleased structure''' The entry 8tkf is ON HOLD until sometime in the future Authors: Paknejad, N., Sapuru, V., Hite, R.K. Description: Human Type 3 IP3 Receptor -Activated State ...

New page

'''Unreleased structure'''

The entry 8tkf is ON HOLD until sometime in the future

Authors: Paknejad, N., Sapuru, V., Hite, R.K.

Description: Human Type 3 IP3 Receptor -Activated State (+IP3/ATP/JD Ca2+)
[[Category: Unreleased Structures]]
[[Category: Paknejad, N]]
[[Category: Hite, R.K]]
[[Category: Sapuru, V]]

8tkf - Revision history

OCA at 12:08, 8 November 2023

OCA at 10:48, 1 November 2023

OCA: Protected "8tkf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8tkf is ON HOLD until sometime in the future Authors: Paknejad, N., Sapuru, V., Hite, R.K. Description: Human Type 3 IP3 Receptor -Activated State ...