8won - Revision history

OCA at 13:58, 1 January 2025

2025-01-01T13:58:54Z

← Older revision		Revision as of 13:58, 1 January 2025
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	~~'''Unreleased structure'''~~

	The ~~entry 8won~~ is ~~ON HOLD~~		==Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis==
			<StructureSection load='8won' size='340' side='right'caption='[[8won]], [[Resolution\|resolution]] 2.69Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8won]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WON FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.69Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8won FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8won OCA], [https://pdbe.org/8won PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8won RCSB], [https://www.ebi.ac.uk/pdbsum/8won PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8won ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A653FP42_MYCSM A0A653FP42_MYCSM]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Encapsulin nanocompartments loaded with dedicated cargo proteins via unique targeting peptides, play a key role in stress resistance, iron storage and natural product biosynthesis. Mmp1 and cysteine desulfurase (Enc-CD) have been identified as the most abundant representatives of family 2 encapsulin systems. However, the molecular assembly, catalytic mechanism, and physiological functions of the Mmp1 encapsulin system have not been studied in detail. Here we isolate and characterize an Enc-CD-loaded Mmp1 encapsulin system from Mycobacterium smegmatis mc(2)155. The cryo-EM structure of the Mmp1 encapsulin and the crystal structure of the naked cargo Enc-CD have been determined. The structure shows that the Mmp1 protomer assembles two conformation models, the icosahedron (T = 1) and homodecamer, with the resolution of 2.60 A and 2.69 A. The Enc-CD at 2.10 A resolution is dimeric and loaded into the Mmp1 (T = 1) encapsulin through the N-terminal long disordered region. Mmp1 encapsulin protects Enc-CD against oxidation as well as to maintain structural stability. These studies provide new insights into the mechanism by which Enc-CD-loaded encapsulin stores sulfur and provides a framework for discovery of new anti-mycobacterial therapeutics.

	~~Authors:~~ Zhang, M., ~~Tang~~, Y., ~~Gao~~, Y., ~~Liu~~, X., ~~Lan~~, W., Liu, Y.~~, Ma, M~~.		The structural and functional analysis of mycobacteria cysteine desulfurase-loaded encapsulin.,Tang Y, Liu Y, Zhang M, Lan W, Ma M, Chen C, Wu S, Chen R, Yan Y, Feng L, Li Y, Guddat LW, Gao Y, Liu X, Rao Z Commun Biol. 2024 Dec 19;7(1):1656. doi: 10.1038/s42003-024-07299-8. PMID:39702509<ref>PMID:39702509</ref>

	~~Description: Cryo-EM structure~~ of the 10-~~subunits Mmp1 complex from Mycobacterium smegmatis~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Ma, M~~]]		<div class="pdbe-citations 8won" style="background-color:#fffaf0;"></div>
	[[Category: ~~Liu,~~ Y]]		== References ==
	[[Category: ~~Zhang, M~~]]		<references/>
	[[Category: ~~Gao,~~ Y]]		__TOC__
	[[Category: ~~Tang, Y~~]]		</StructureSection>
	[[Category: ~~Lan, W~~]]		[[Category: Large Structures]]
	[[Category: ~~Liu, X~~]]		[[Category: Mycolicibacterium smegmatis]]
			[[Category: Gao Y]]
			[[Category: Lan W]]
			[[Category: Liu X]]
			[[Category: Liu Y]]
			[[Category: Ma M]]
			[[Category: Tang Y]]
			[[Category: Zhang M]]

OCA at 04:41, 27 November 2024

2024-11-27T04:41:19Z

← Older revision		Revision as of 04:41, 27 November 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8won is ON HOLD ~~until Paper Publication~~		The entry 8won is ON HOLD

	Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M.		Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M.
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	Description: Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis		Description: Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Ma, M]]
			[[Category: Liu, Y]]
	[[Category: Zhang, M]]		[[Category: Zhang, M]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
	~~[[Category: Liu, Y]]~~
	[[Category: Tang, Y]]		[[Category: Tang, Y]]
			[[Category: Lan, W]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
	~~[[Category: Lan, W]]~~
	~~[[Category: Ma, M]]~~

OCA at 12:28, 29 May 2024

2024-05-29T12:28:00Z

← Older revision		Revision as of 12:28, 29 May 2024
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	Description: Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis		Description: Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Zhang, M]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
			[[Category: Liu, Y]]
	[[Category: Tang, Y]]		[[Category: Tang, Y]]
			[[Category: Liu, X]]
	[[Category: Lan, W]]		[[Category: Lan, W]]
	~~[[Category: Liu, X]]~~
	~~[[Category: Zhang, M]]~~
	~~[[Category: Liu, Y]]~~
	[[Category: Ma, M]]		[[Category: Ma, M]]

OCA at 22:18, 27 December 2023

2023-12-27T22:18:01Z

← Older revision		Revision as of 22:18, 27 December 2023
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8won is ON HOLD		The entry 8won is ON HOLD until Paper Publication

	Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M.		Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M.

OCA: Protected "8won" [edit=sysop:move=sysop]

2023-10-18T12:43:23Z

Protected "8won" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:43, 18 October 2023
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8won is ON HOLD Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M. Description: Cryo-EM structure of the 10-subunits Mmp1 com...

2023-10-18T12:43:21Z

New page: '''Unreleased structure''' The entry 8won is ON HOLD Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M. Description: Cryo-EM structure of the 10-subunits Mmp1 com...

New page

'''Unreleased structure'''

The entry 8won is ON HOLD

Authors: Zhang, M., Tang, Y., Gao, Y., Liu, X., Lan, W., Liu, Y., Ma, M.

Description: Cryo-EM structure of the 10-subunits Mmp1 complex from Mycobacterium smegmatis
[[Category: Unreleased Structures]]
[[Category: Gao, Y]]
[[Category: Tang, Y]]
[[Category: Lan, W]]
[[Category: Liu, X]]
[[Category: Zhang, M]]
[[Category: Liu, Y]]
[[Category: Ma, M]]