OCA at 09:30, 25 December 2024

2024-12-25T09:30:26Z

← Older revision		Revision as of 09:30, 25 December 2024
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 8zos is ~~ON HOLD until Paper~~ Publication		==Cryo-EM structure of pyraclostrobin-bound porcine bc1 complex==
			<StructureSection load='8zos' size='340' side='right'caption='[[8zos]], [[Resolution\|resolution]] 2.37Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[8zos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZOS FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.37Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1D6K:methyl+~{N}-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-~{N}-methoxy-carbamate'>A1D6K</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zos OCA], [https://pdbe.org/8zos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zos RCSB], [https://www.ebi.ac.uk/pdbsum/8zos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zos ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/F1SDI2_PIG F1SDI2_PIG]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cytochrome bc(1) (complex III) represents a significant target for the discovery of both drugs and fungicides. Metyltetraprole (MET) is commonly classified as a quinone site inhibitor (Q(o)I) that combats the G143A mutated isolate, which confers high resistance to strobilurin fungicides such as pyraclostrobin (PYR). The binding mode and antiresistance mechanism of MET remain unclear. Here, we determined the high-resolution structures of inhibitor-bound S. cerevisiae complex III (MET, 2.52 A; PYR, 2.42 A) and inhibitor-bound porcine complex III (MET, 2.53 A; PYR, 2,37 A) by cryo-electron microscopy. The distinct binding modes of MET and PYR were observed for the first time. Notably, the MET exhibited different binding modes in the two species. In S. cerevisiae, the binding site of MET was the same as PYR, serving as a Pm-type inhibitor of the Q(o) site. However, in porcine, MET acted as a dual-target inhibitor of both Q(o) and Q(i). Based on the structural insights, a novel inhibitor (YF23694) was discovered and demonstrated excellent fungicidal activity against downy mildew and powdery mildew fungi. This work provides a new starting point for the design of the next generation of inhibitors to overcome the resistance.

	~~Authors:~~ Wang, Y~~.X.~~, Sun, J.Y.~~, Li, Z.W~~.~~, Cui, G~~.R., ~~Yang, G.F~~.		Cryo-EM Structures Reveal the Unique Binding Modes of Metyltetraprole in Yeast and Porcine Cytochrome bc(1) Complex Enabling Rational Design of Inhibitors.,Wang YX, Ye Y, Li ZW, Cui GR, Shi XX, Dong Y, Jiang JJ, Sun JY, Guan ZW, Zhang N, Wu QY, Wang F, Zhu XL, Yang GF J Am Chem Soc. 2024 Dec 11;146(49):33903-33913. doi: 10.1021/jacs.4c12595. Epub , 2024 Nov 27. PMID:39601138<ref>PMID:39601138</ref>

	~~Description: Cryo~~-~~EM structure of pyraclostrobin~~-~~bound porcine bc1 complex~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: Cui~~, G.R~~]]		<div class="pdbe-citations 8zos" style="background-color:#fffaf0;"></div>
	[[Category: ~~Yang, G.F~~]]		== References ==
	[[Category: ~~Wang, Y.X~~]]		<references/>
	[[Category: ~~Li, Z.W~~]]		__TOC__
	[[Category: ~~Sun, J.Y~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Sus scrofa]]
			[[Category: Cui GR]]
			[[Category: Li ZW]]
			[[Category: Sun JY]]
			[[Category: Wang YX]]
			[[Category: Yang GF]]

OCA at 04:37, 19 June 2024

2024-06-19T04:37:42Z

← Older revision		Revision as of 04:37, 19 June 2024
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 8zos is ON HOLD		The entry 8zos is ON HOLD until Paper Publication

	Authors: Wang, Y.X., Sun, J.L., Li, Z.W., Cui, G.R., Yang, G.F.		Authors: Wang, Y.X., Sun, J.Y., Li, Z.W., Cui, G.R., Yang, G.F.

	Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 complex		Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Li, Z.W]]		[[Category: Cui, G.R]]
	[[Category: Yang, G.F]]		[[Category: Yang, G.F]]
	~~[[Category: Sun, J.L]]~~
	[[Category: Wang, Y.X]]		[[Category: Wang, Y.X]]
	[[Category: ~~Cui~~, G.R]]		[[Category: Li, Z.W]]
			[[Category: Sun, J.Y]]

OCA: Protected "8zos" [edit=sysop:move=sysop]

2024-06-05T04:13:55Z

Protected "8zos" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:13, 5 June 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 8zos is ON HOLD Authors: Wang, Y.X., Sun, J.L., Li, Z.W., Cui, G.R., Yang, G.F. Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 ...

2024-06-05T04:13:54Z

New page: '''Unreleased structure''' The entry 8zos is ON HOLD Authors: Wang, Y.X., Sun, J.L., Li, Z.W., Cui, G.R., Yang, G.F. Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 ...

New page

'''Unreleased structure'''

The entry 8zos is ON HOLD

Authors: Wang, Y.X., Sun, J.L., Li, Z.W., Cui, G.R., Yang, G.F.

Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 complex
[[Category: Unreleased Structures]]
[[Category: Li, Z.W]]
[[Category: Yang, G.F]]
[[Category: Sun, J.L]]
[[Category: Wang, Y.X]]
[[Category: Cui, G.R]]

8zos - Revision history

OCA at 09:30, 25 December 2024

OCA at 04:37, 19 June 2024

OCA: Protected "8zos" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 8zos is ON HOLD Authors: Wang, Y.X., Sun, J.L., Li, Z.W., Cui, G.R., Yang, G.F. Description: Cryo-EM structure of pyraclostrobin-bound porcine bc1 ...