OCA at 11:16, 16 April 2025

2025-04-16T11:16:07Z

← Older revision		Revision as of 11:16, 16 April 2025
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9bea is ~~ON HOLD~~		==SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation==
			<StructureSection load='9bea' size='340' side='right'caption='[[9bea]], [[Resolution\|resolution]] 3.85Å' scene=''>
	~~Authors~~: ~~Singh~~, S., ~~Hasan~~, S.S.		== Structural highlights ==
			<table><tr><td colspan='2'>[[9bea]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BEA FirstGlance]. <br>
	~~Description~~: ~~SARS CoV~~-~~2 full~~-~~length spike protein with internal tag~~, ~~2RBD~~-~~up conformation~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.85Å</td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	[[Category: Hasan~~, S.S~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bea OCA], [https://pdbe.org/9bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bea RCSB], [https://www.ebi.ac.uk/pdbsum/9bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bea ProSAT]</span></td></tr>
	[[Category: Singh, S]]		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Severe acute respiratory syndrome coronavirus 2]]
			[[Category: Hasan SS]]
			[[Category: Singh S]]

OCA at 14:47, 29 May 2024

2024-05-29T14:47:41Z

← Older revision		Revision as of 14:47, 29 May 2024
Line 7:		Line 7:
	Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation		Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Hasan, S.S]]
	[[Category: Singh, S]]		[[Category: Singh, S]]
	~~[[Category: Hasan, S.S]]~~

OCA: Protected "9bea" [edit=sysop:move=sysop]

2024-05-01T05:12:00Z

Protected "9bea" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:12, 1 May 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9bea is ON HOLD Authors: Singh, S., Hasan, S.S. Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation [[Categor...

2024-05-01T05:11:59Z

New page: '''Unreleased structure''' The entry 9bea is ON HOLD Authors: Singh, S., Hasan, S.S. Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation [[Categor...

New page

'''Unreleased structure'''

The entry 9bea is ON HOLD

Authors: Singh, S., Hasan, S.S.

Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation
[[Category: Unreleased Structures]]
[[Category: Singh, S]]
[[Category: Hasan, S.S]]

9bea - Revision history

OCA at 11:16, 16 April 2025

OCA at 14:47, 29 May 2024

OCA: Protected "9bea" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9bea is ON HOLD Authors: Singh, S., Hasan, S.S. Description: SARS CoV-2 full-length spike protein with internal tag, 2RBD-up conformation [[Categor...