OCA at 06:31, 18 December 2024

2024-12-18T06:31:33Z

← Older revision		Revision as of 06:31, 18 December 2024
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9d4e is ~~ON HOLD~~		==Crystal structure of the human DCAF1 WDR domain in complex with OICR-41103==
			<StructureSection load='9d4e' size='340' side='right'caption='[[9d4e]], [[Resolution\|resolution]] 1.70Å' scene=''>
	~~Authors~~: ~~Kimani~~, S., ~~Dong~~, A., Li, Y., ~~Seitova, A~~.~~, Al~~-~~awar, R~~., ~~Mamai~~, A., ~~Ackloo~~, S., ~~Arrowsmith~~, ~~C.H~~., ~~Edwards~~, A.M., ~~Halabelian, L~~.~~, Structural Genomics Consortium~~ (~~SGC~~)		== Structural highlights ==
			<table><tr><td colspan='2'>[[9d4e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9D4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9D4E FirstGlance]. <br>
	~~Description~~: ~~Crystal structure~~ of the ~~human~~ DCAF1 ~~WDR domain~~ in complex ~~with OICR~~-~~1103~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1A1Q:~{N}-[(1~{S})-3-azanyl-1-(3-chloranyl-4-fluoranyl-phenyl)-3-oxidanylidene-propyl]-4-(4-chloranyl-2-fluoranyl-phenyl)-5-[(~{E})-3-oxidanylidene-3-piperidin-1-yl-prop-1-enyl]-1~{H}-pyrrole-3-carboxamide'>A1A1Q</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
	[[Category: ~~Mamai, A~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9d4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9d4e OCA], [https://pdbe.org/9d4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9d4e RCSB], [https://www.ebi.ac.uk/pdbsum/9d4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9d4e ProSAT]</span></td></tr>
	[[Category: ~~Arrowsmith, C.H~~]]		</table>
	[[Category: ~~Ackloo, S~~]]		== Function ==
	[[Category: ~~Structural Genomics Consortium (Sgc)~~]]		[https://www.uniprot.org/uniprot/DCAF1_HUMAN DCAF1_HUMAN] Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).<ref>PMID:16964240</ref> <ref>PMID:17609381</ref> <ref>PMID:17630831</ref> <ref>PMID:18332868</ref> <ref>PMID:18524771</ref> <ref>PMID:18606781</ref> <ref>PMID:19287380</ref> <ref>PMID:20644714</ref> <ref>PMID:22184063</ref> <ref>PMID:23063525</ref> <ref>PMID:23362280</ref> <ref>PMID:24140421</ref> (Microbial infection) In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. The HIV-1 Vpr protein hijacks the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex to promote ubiquitination and degradation of proteins such as TERT and ZIP/ZGPAT.<ref>PMID:17314515</ref> <ref>PMID:17559673</ref> <ref>PMID:17609381</ref> <ref>PMID:17620334</ref> <ref>PMID:17626091</ref> <ref>PMID:17630831</ref> <ref>PMID:18524771</ref> <ref>PMID:24116224</ref> (Microbial infection) In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage.<ref>PMID:17314515</ref> <ref>PMID:18464893</ref> <ref>PMID:19264781</ref> <ref>PMID:19923175</ref> <ref>PMID:24336198</ref>
	[[Category: ~~Edwards,~~ A.M]]		== References ==
	[[Category: ~~Dong, A~~]]		<references/>
	[[Category: Halabelian, L]]		__TOC__
	[[Category: ~~Li, Y~~]]		</StructureSection>
	[[Category: ~~Kimani, S~~]]		[[Category: Homo sapiens]]
	[[Category: ~~Al-Awar, R~~]]		[[Category: Large Structures]]
	[[Category: Seitova, A]]		[[Category: Ackloo S]]
			[[Category: Al-awar R]]
			[[Category: Arrowsmith CH]]
			[[Category: Dong A]]
			[[Category: Edwards AM]]
			[[Category: Halabelian L]]
			[[Category: Kimani S]]
			[[Category: Li Y]]
			[[Category: Mamai A]]
			[[Category: Seitova A]]

OCA: Protected "9d4e" [edit=sysop:move=sysop]

2024-08-21T12:05:44Z

Protected "9d4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:05, 21 August 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9d4e is ON HOLD Authors: Kimani, S., Dong, A., Li, Y., Seitova, A., Al-awar, R., Mamai, A., Ackloo, S., Arrowsmith, C.H., Edwards, A.M., Halabelian,...

2024-08-21T12:05:43Z

New page: '''Unreleased structure''' The entry 9d4e is ON HOLD Authors: Kimani, S., Dong, A., Li, Y., Seitova, A., Al-awar, R., Mamai, A., Ackloo, S., Arrowsmith, C.H., Edwards, A.M., Halabelian,...

New page

'''Unreleased structure'''

The entry 9d4e is ON HOLD

Authors: Kimani, S., Dong, A., Li, Y., Seitova, A., Al-awar, R., Mamai, A., Ackloo, S., Arrowsmith, C.H., Edwards, A.M., Halabelian, L., Structural Genomics Consortium (SGC)

Description: Crystal structure of the human DCAF1 WDR domain in complex with OICR-1103
[[Category: Unreleased Structures]]
[[Category: Mamai, A]]
[[Category: Arrowsmith, C.H]]
[[Category: Ackloo, S]]
[[Category: Structural Genomics Consortium (Sgc)]]
[[Category: Edwards, A.M]]
[[Category: Dong, A]]
[[Category: Halabelian, L]]
[[Category: Li, Y]]
[[Category: Kimani, S]]
[[Category: Al-Awar, R]]
[[Category: Seitova, A]]

9d4e - Revision history

OCA at 06:31, 18 December 2024

OCA: Protected "9d4e" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9d4e is ON HOLD Authors: Kimani, S., Dong, A., Li, Y., Seitova, A., Al-awar, R., Mamai, A., Ackloo, S., Arrowsmith, C.H., Edwards, A.M., Halabelian,...