9ext - Revision history

OCA at 07:35, 27 November 2024

2024-11-27T07:35:49Z

← Older revision		Revision as of 07:35, 27 November 2024
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9ext is ~~ON HOLD until Paper~~ Publication		==Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)==
			<StructureSection load='9ext' size='340' side='right'caption='[[9ext]], [[Resolution\|resolution]] 2.75Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[9ext]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EXT FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ext FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ext OCA], [https://pdbe.org/9ext PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ext RCSB], [https://www.ebi.ac.uk/pdbsum/9ext PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ext ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CLH_YEAST CLH_YEAST] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. In yeast, it is involved in the retention of proteins in an intracellular membrane compartment, presumably the trans-Golgi.<ref>PMID:12686590</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Clathrin forms a triskelion, or three-legged, network that regulates cellular processes by facilitating cargo internalization and trafficking in eukaryotes. Its N-terminal domain is crucial for interacting with adaptor proteins, which link clathrin to the membrane and engage with specific cargo. The N-terminal domain contains up to four adaptor-binding sites, though their role in preferential occupancy by adaptor proteins remains unclear. In this study, we examine the binding hierarchy of adaptors for clathrin, using integrative biophysical and structural approaches, along with in vivo functional experiments. We find that yeast epsin Ent5 has the highest affinity for clathrin, highlighting its key role in cellular trafficking. Epsins Ent1 and Ent2, crucial for endocytosis but thought to have redundant functions, show distinct binding patterns. Ent1 exhibits stronger interactions with clathrin than Ent2, suggesting a functional divergence toward actin binding. These results offer molecular insights into adaptor protein selectivity, suggesting they competitively bind clathrin while also targeting three different clathrin sites.

	~~Authors:~~ Defelipe, ~~L.A.~~, Bento, I., Garcia Alai~~, M~~.M.		Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.,Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kolbel K, Uetrecht C, Thuenauer R, Garcia-Alai MM Nat Commun. 2024 Nov 7;15(1):9655. doi: 10.1038/s41467-024-54037-z. PMID:39511183<ref>PMID:39511183</ref>

	~~Description: Crystal structure~~ of ~~Yeast Clathrin Heavy Chain N~~-~~terminal domain bound to APL2~~/~~AP-1 Beta peptide (LLDL)~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Garcia Alai, M.M~~]]		<div class="pdbe-citations 9ext" style="background-color:#fffaf0;"></div>
	[[Category: ~~Defelipe, L.A~~]]		== References ==
	[[Category: Bento, I]]		<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Saccharomyces cerevisiae]]
			[[Category: Saccharomyces cerevisiae S288C]]
			[[Category: Bento I]]
			[[Category: Defelipe LA]]
			[[Category: Garcia Alai MM]]

OCA at 15:23, 29 May 2024

2024-05-29T15:23:33Z

← Older revision		Revision as of 15:23, 29 May 2024
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	Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)		Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Garcia Alai, M.M]]
	[[Category: Defelipe, L.A]]		[[Category: Defelipe, L.A]]
	~~[[Category: Garcia Alai, M.M]]~~
	[[Category: Bento, I]]		[[Category: Bento, I]]

OCA at 04:23, 15 May 2024

2024-05-15T04:23:57Z

← Older revision		Revision as of 04:23, 15 May 2024
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 9ext is ON HOLD		The entry 9ext is ON HOLD until Paper Publication

	Authors: Defelipe, L.A., Bento, I., Garcia Alai, M.M.		Authors: Defelipe, L.A., Bento, I., Garcia Alai, M.M.
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	Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)		Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Defelipe, L.A]]
	[[Category: Garcia Alai, M.M]]		[[Category: Garcia Alai, M.M]]
	[[Category: Bento, I]]		[[Category: Bento, I]]
	~~[[Category: Defelipe, L.A]]~~

OCA: Protected "9ext" [edit=sysop:move=sysop]

2024-04-17T04:40:28Z

Protected "9ext" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:40, 17 April 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9ext is ON HOLD Authors: Defelipe, L.A., Bento, I., Garcia Alai, M.M. Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domai...

2024-04-17T04:40:27Z

New page: '''Unreleased structure''' The entry 9ext is ON HOLD Authors: Defelipe, L.A., Bento, I., Garcia Alai, M.M. Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domai...

New page

'''Unreleased structure'''

The entry 9ext is ON HOLD

Authors: Defelipe, L.A., Bento, I., Garcia Alai, M.M.

Description: Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to APL2/AP-1 Beta peptide (LLDL)
[[Category: Unreleased Structures]]
[[Category: Garcia Alai, M.M]]
[[Category: Bento, I]]
[[Category: Defelipe, L.A]]