OCA at 13:11, 21 August 2024

2024-08-21T13:11:21Z

← Older revision		Revision as of 13:11, 21 August 2024
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9fcd is ~~ON HOLD until Paper~~ Publication		==CysG(N-16) in complex with SAH from Kitasatospora cystarginea==
			<StructureSection load='9fcd' size='340' side='right'caption='[[9fcd]], [[Resolution\|resolution]] 1.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[9fcd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_cystarginea Kitasatospora cystarginea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FCD FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fcd OCA], [https://pdbe.org/9fcd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fcd RCSB], [https://www.ebi.ac.uk/pdbsum/9fcd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fcd ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A1W6R556_9ACTN A0A1W6R556_9ACTN]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cystargolides and belactosins are natural products with a distinct dipeptide structure and an electrophilic beta-lactone warhead. They are known to inhibit proteases such as the proteasome or caseinolytic protease P, highlighting their potential in treating cancers and neurodegenerative diseases. Recent genetic analyses have shown homology between the biosynthetic pathways of the two inhibitors. Here, we characterize the O-methyltransferases BelI and CysG, which catalyze the initial step of beta-lactone formation. Employing techniques such as crystallography, computational analysis, mutagenesis, and activity assays, we identified a His-His-Asp (HHD) motif in the active sites of the two enzymes, which is crucial for binding a catalytically active calcium ion. Our findings thus elucidate a conserved divalent metal-dependent mechanism in both biosynthetic pathways that distinguishes BelI and CysG from previously characterized O-methyltransferases.

	~~Authors~~: Kuttenlochner, W., Beller, P., Kaysser, L., Groll, M.		Deciphering the SAM- and Metal-Dependent Mechanism of O-Methyltransferases in Cystargolide and Belactosin Biosynthesis: A Structure-Activity Relationship Study.,Kuttenlochner W, Beller P, Kaysser L, Groll M J Biol Chem. 2024 Aug 7:107646. doi: 10.1016/j.jbc.2024.107646. PMID:39121999<ref>PMID:39121999</ref>

	~~Description~~: ~~CysG(N-16) in complex with SAH from~~ Kitasatospora cystarginea		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Kaysser, L~~]]		<div class="pdbe-citations 9fcd" style="background-color:#fffaf0;"></div>
	[[Category: ~~Beller, P~~]]		== References ==
	[[Category: ~~Kuttenlochner, W~~]]		<references/>
	[[Category: ~~Groll, M~~]]		__TOC__
			</StructureSection>
			[[Category: Kitasatospora cystarginea]]
			[[Category: Large Structures]]
			[[Category: Beller P]]
			[[Category: Groll M]]
			[[Category: Kaysser L]]
			[[Category: Kuttenlochner W]]

OCA at 15:38, 29 May 2024

2024-05-29T15:38:50Z

← Older revision		Revision as of 15:38, 29 May 2024
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	Description: CysG(N-16) in complex with SAH from Kitasatospora cystarginea		Description: CysG(N-16) in complex with SAH from Kitasatospora cystarginea
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Kaysser, L]]
			[[Category: Beller, P]]
	[[Category: Kuttenlochner, W]]		[[Category: Kuttenlochner, W]]
	~~[[Category: Beller, P]]~~
	~~[[Category: Kaysser, L]]~~
	[[Category: Groll, M]]		[[Category: Groll, M]]

OCA: Protected "9fcd" [edit=sysop:move=sysop]

2024-05-22T07:12:54Z

Protected "9fcd" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:12, 22 May 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9fcd is ON HOLD until Paper Publication Authors: Kuttenlochner, W., Beller, P., Kaysser, L., Groll, M. Description: CysG(N-16) in complex with SAH ...

2024-05-22T07:12:53Z

New page: '''Unreleased structure''' The entry 9fcd is ON HOLD until Paper Publication Authors: Kuttenlochner, W., Beller, P., Kaysser, L., Groll, M. Description: CysG(N-16) in complex with SAH ...

New page

'''Unreleased structure'''

The entry 9fcd is ON HOLD until Paper Publication

Authors: Kuttenlochner, W., Beller, P., Kaysser, L., Groll, M.

Description: CysG(N-16) in complex with SAH from Kitasatospora cystarginea
[[Category: Unreleased Structures]]
[[Category: Kuttenlochner, W]]
[[Category: Beller, P]]
[[Category: Kaysser, L]]
[[Category: Groll, M]]

9fcd - Revision history

OCA at 13:11, 21 August 2024

OCA at 15:38, 29 May 2024

OCA: Protected "9fcd" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9fcd is ON HOLD until Paper Publication Authors: Kuttenlochner, W., Beller, P., Kaysser, L., Groll, M. Description: CysG(N-16) in complex with SAH ...