OCA at 04:20, 17 October 2024

2024-10-17T04:20:22Z

← Older revision		Revision as of 04:20, 17 October 2024
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9fvs is ~~ON HOLD~~		==Crystal structure of Heme-Oxygenase mutant G139A from Corynebacterium diphtheriae complexed with Cobalt-porphyrine (HumO-Co(III))==
			<StructureSection load='9fvs' size='340' side='right'caption='[[9fvs]], [[Resolution\|resolution]] 1.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[9fvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FVS FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fvs OCA], [https://pdbe.org/9fvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fvs RCSB], [https://www.ebi.ac.uk/pdbsum/9fvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fvs ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q54AI1_CORDP Q54AI1_CORDP]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Light-dependent reduction of carbon dioxide (CO(2)) into value-added products can be catalyzed by a variety of molecular complexes. Here we report a rare example of a structurally characterized artificial enzyme, resulting from the combination of a heme binding protein, heme oxygenase, with cobalt-protoporphyrin IX, with good activity for the photoreduction of CO(2) to carbon monoxide (CO). Using a copper-based photosensitizer, thus making the photosystem free of noble metals, a large turnover frequency value of approximately 616 h(-1), a turnover value of approximately 589, after 3 h reaction, and a CO vs H(2) selectivity of 72% were obtained, establishing a record among previously reported artificial CO(2) reductases. Thorough photophysical studies allowed tracking of reaction intermediates and provided insights into the reaction mechanism. Thanks to a high-resolution crystal structure of the artificial enzyme, both in the absence and in the presence of the protein-bound CO(2) substrate, a rational site-directed mutagenesis approach was used to study the effect of some modifications of the active site on the activity.

	~~Authors~~: Labidi~~, R.J.~~, Faivre, B., Carpentier, P., Perard, J., Gotico, P., Li, Y., Atta, M., Fontecave, M.		Light-Activated Artificial CO(2)-Reductase: Structure and Activity.,Labidi RJ, Faivre B, Carpentier P, Perard J, Gotico P, Li Y, Atta M, Fontecave M J Am Chem Soc. 2024 Oct 1. doi: 10.1021/jacs.4c08927. PMID:39352411<ref>PMID:39352411</ref>

	~~Description: Crystal structure~~ of ~~Heme~~-~~Oxygenase mutant G139A from Corynebacterium diphtheriae complexed with Cobalt~~-~~porphyrine (HumO-Co(III))~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
	[[Category: ~~Faivre, B~~]]		<div class="pdbe-citations 9fvs" style="background-color:#fffaf0;"></div>
	[[Category: ~~Fontecave,~~ M]]		== References ==
	[[Category: Carpentier, P]]		<references/>
	[[Category: ~~Atta,~~ M]]		__TOC__
	[[Category: ~~Perard, J~~]]		</StructureSection>
	[[Category: ~~Li, Y~~]]		[[Category: Corynebacterium diphtheriae]]
	[[Category: ~~Gotico, P~~]]		[[Category: Large Structures]]
	[[Category: ~~Labidi, R.~~J]]		[[Category: Atta M]]
			[[Category: Carpentier P]]
			[[Category: Faivre B]]
			[[Category: Fontecave M]]
			[[Category: Gotico P]]
			[[Category: Labidi RJ]]
			[[Category: Li Y]]
			[[Category: Perard J]]

OCA: Protected "9fvs" [edit=sysop:move=sysop]

2024-07-03T06:17:28Z

Protected "9fvs" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:17, 3 July 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9fvs is ON HOLD Authors: Labidi, R.J., Faivre, B., Carpentier, P., Perard, J., Gotico, P., Li, Y., Atta, M., Fontecave, M. Description: Crystal str...

2024-07-03T06:17:27Z

New page: '''Unreleased structure''' The entry 9fvs is ON HOLD Authors: Labidi, R.J., Faivre, B., Carpentier, P., Perard, J., Gotico, P., Li, Y., Atta, M., Fontecave, M. Description: Crystal str...

New page

'''Unreleased structure'''

The entry 9fvs is ON HOLD

Authors: Labidi, R.J., Faivre, B., Carpentier, P., Perard, J., Gotico, P., Li, Y., Atta, M., Fontecave, M.

Description: Crystal structure of Heme-Oxygenase mutant G139A from Corynebacterium diphtheriae complexed with Cobalt-porphyrine (HumO-Co(III))
[[Category: Unreleased Structures]]
[[Category: Faivre, B]]
[[Category: Fontecave, M]]
[[Category: Carpentier, P]]
[[Category: Atta, M]]
[[Category: Perard, J]]
[[Category: Li, Y]]
[[Category: Gotico, P]]
[[Category: Labidi, R.J]]

9fvs - Revision history

OCA at 04:20, 17 October 2024

OCA: Protected "9fvs" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9fvs is ON HOLD Authors: Labidi, R.J., Faivre, B., Carpentier, P., Perard, J., Gotico, P., Li, Y., Atta, M., Fontecave, M. Description: Crystal str...