9k4e - Revision history

OCA at 09:14, 22 October 2025

2025-10-22T09:14:21Z

← Older revision		Revision as of 09:14, 22 October 2025
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9k4e is ~~ON HOLD until Paper Publication~~		==SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class1==
			<StructureSection load='9k4e' size='340' side='right'caption='[[9k4e]], [[Resolution\|resolution]] 3.30Å' scene=''>
	~~Authors~~: ~~Zheng~~, R., Ma, L.J.		== Structural highlights ==
			<table><tr><td colspan='2'>[[9k4e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9K4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9K4E FirstGlance]. <br>
	~~Description~~: ~~SuperFi~~ Cas9 -~~mismatch 22nt sgRNA~~ -~~DNA ternary complex class1~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9k4e OCA], [https://pdbe.org/9k4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9k4e RCSB], [https://www.ebi.ac.uk/pdbsum/9k4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9k4e ProSAT]</span></td></tr>
	[[Category: Ma~~, L.J~~]]		</table>
	[[Category: Zheng, R]]		== Function ==
			[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Streptococcus pyogenes]]
			[[Category: Synthetic construct]]
			[[Category: Ma LJ]]
			[[Category: Zheng R]]

OCA at 05:33, 27 August 2025

2025-08-27T05:33:22Z

← Older revision		Revision as of 05:33, 27 August 2025
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 9k4e is ON HOLD		The entry 9k4e is ON HOLD until Paper Publication

	Authors: Zheng, R., Ma, L.J.		Authors: Zheng, R., Ma, L.J.

OCA at 04:48, 20 August 2025

2025-08-20T04:48:50Z

← Older revision		Revision as of 04:48, 20 August 2025
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 9k4e is ON HOLD ~~until Paper Publication~~		The entry 9k4e is ON HOLD

	Authors: Zheng, R., Ma, L.J.		Authors: Zheng, R., Ma, L.J.
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	Description: SuperFi Cas9 -mismatch 22nt sgRNA -DNA ternary complex class1		Description: SuperFi Cas9 -mismatch 22nt sgRNA -DNA ternary complex class1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Ma, L.J]]
	[[Category: Zheng, R]]		[[Category: Zheng, R]]
	~~[[Category: Ma, L.J]]~~

OCA at 06:18, 9 April 2025

2025-04-09T06:18:50Z

← Older revision		Revision as of 06:18, 9 April 2025
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 9k4e is ON HOLD		The entry 9k4e is ON HOLD until Paper Publication

	Authors: Zheng, R., Ma, L.J.		Authors: Zheng, R., Ma, L.J.

OCA: Protected "9k4e" [edit=sysop:move=sysop]

2024-10-30T05:17:22Z

Protected "9k4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:17, 30 October 2024
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9k4e is ON HOLD Authors: Zheng, R., Ma, L.J. Description: SuperFi Cas9 -mismatch 22nt sgRNA -DNA ternary complex class1 [[Category: Unreleased Stru...

2024-10-30T05:17:20Z

New page: '''Unreleased structure''' The entry 9k4e is ON HOLD Authors: Zheng, R., Ma, L.J. Description: SuperFi Cas9 -mismatch 22nt sgRNA -DNA ternary complex class1 [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 9k4e is ON HOLD

Authors: Zheng, R., Ma, L.J.

Description: SuperFi Cas9 -mismatch 22nt sgRNA -DNA ternary complex class1
[[Category: Unreleased Structures]]
[[Category: Zheng, R]]
[[Category: Ma, L.J]]