OCA at 09:03, 11 March 2026

2026-03-11T09:03:58Z

← Older revision		Revision as of 09:03, 11 March 2026
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	~~'''Unreleased structure'''~~

	~~The entry~~ 9o7u is ~~ON HOLD until Paper~~ Publication		==Structure Determination of Pedobacter sp. KP-2 PahZ1==
			<StructureSection load='9o7u' size='340' side='right'caption='[[9o7u]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[9o7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pedobacter_sp._KP-2 Pedobacter sp. KP-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9O7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9O7U FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9o7u OCA], [https://pdbe.org/9o7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9o7u RCSB], [https://www.ebi.ac.uk/pdbsum/9o7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9o7u ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Polyaspartic acid (PAA) is a biodegradable polymer with various industrial applications. To date there are only three known PAA hydrolases (from the gene PahZ) capable of degrading PAA . These enzymes are expressed in two different bacteria, Sphingomonas sp. KT-1 (PahZ1KT-1 and PahZ2KT-1) and Pedobacter sp. KP-2 (PahZ1KP-2). PahZ1KT-1 and PahZ2KT-1 form a two-component system degrading tPAA to oligoaspartic acid (OAA) and subsequently into aspartic acid. This study aims to expand the diversity of PAA hydrolases and inform efforts to improve PAA degradation. To further understand the known PahZ1 homologs, the X-ray crystal structure of PahZ1KP-2 was determined to examine its structural homology with PahZ1KT-1. Crystallographic analysis revealed PahZ1KP-2 is monomeric, contrasting with the dimeric PahZ1KT-1, yet both share a conserved serine protease catalytic triad. With the aim of expanding the PahZ1 family, four putative homologs were identified using bioinformatics and AI-based structural modeling, all of which retained the alpha/beta hydrolase domain. Importantly, all homologs exhibited measurable PAA-degrading activity and each was classified as either monomer or dimer to further expand the diversity of PahZ1 enzymes and provide a broader toolbox for sustainable polymer degradation.

	~~Authors:~~ Wallen, J.R.~~, Miller, J~~.M.		Expansion of the known Poly(Aspartic Acid) Hydrolases through the Identification of Four New PahZ1 Homologs.,Marsee JD, Brambley CA, Ho T, Callaway WW, Jansch AL, Taylor K, Watson-Sanders SR, Williams B, Wolvington A, Nguyen CT, Khan L, Cabrera C, Cerna MVC, Wallen JR, Weiland MH, Miller JM Protein Eng Des Sel. 2026 Feb 28:gzag006. doi: 10.1093/protein/gzag006. PMID:41761765<ref>PMID:41761765</ref>

	~~Description: Structure Determination~~ of ~~Pedobacter sp~~. KP-~~2 PahZ1~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: ~~Wallen, J~~.R]]		<div class="pdbe-citations 9o7u" style="background-color:#fffaf0;"></div>
	[[Category: Miller~~, J.M~~]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Pedobacter sp. KP-2]]
			[[Category: Miller JM]]
			[[Category: Wallen JR]]

OCA at 09:18, 30 April 2025

2025-04-30T09:18:30Z

← Older revision		Revision as of 09:18, 30 April 2025
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 9o7u is ON HOLD		The entry 9o7u is ON HOLD until Paper Publication

	Authors: Wallen, J.R., Miller, J.M.		Authors: Wallen, J.R., Miller, J.M.
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	Description: Structure Determination of Pedobacter sp. KP-2 PahZ1		Description: Structure Determination of Pedobacter sp. KP-2 PahZ1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Wallen, J.R]]
	[[Category: Miller, J.M]]		[[Category: Miller, J.M]]
	~~[[Category: Wallen, J.R]]~~

OCA: Protected "9o7u" [edit=sysop:move=sysop]

2025-04-23T04:05:52Z

Protected "9o7u" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:05, 23 April 2025
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9o7u is ON HOLD Authors: Wallen, J.R., Miller, J.M. Description: Structure Determination of Pedobacter sp. KP-2 PahZ1 [[Category: Unreleased Struct...

2025-04-23T04:05:51Z

New page: '''Unreleased structure''' The entry 9o7u is ON HOLD Authors: Wallen, J.R., Miller, J.M. Description: Structure Determination of Pedobacter sp. KP-2 PahZ1 [[Category: Unreleased Struct...

New page

'''Unreleased structure'''

The entry 9o7u is ON HOLD

Authors: Wallen, J.R., Miller, J.M.

Description: Structure Determination of Pedobacter sp. KP-2 PahZ1
[[Category: Unreleased Structures]]
[[Category: Miller, J.M]]
[[Category: Wallen, J.R]]

9o7u - Revision history

OCA at 09:03, 11 March 2026

OCA at 09:18, 30 April 2025

OCA: Protected "9o7u" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9o7u is ON HOLD Authors: Wallen, J.R., Miller, J.M. Description: Structure Determination of Pedobacter sp. KP-2 PahZ1 [[Category: Unreleased Struct...