OCA at 07:36, 8 October 2025

2025-10-08T07:36:46Z

← Older revision		Revision as of 07:36, 8 October 2025
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	~~'''Unreleased structure'''~~

	The ~~entry 9v21~~ is ~~ON HOLD~~		==Cryo- EM structure of 75S ribosome with P- tRNA from Entamoeba histolytica==
			<StructureSection load='9v21' size='340' side='right'caption='[[9v21]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[9v21]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica_HM-1:IMSS Entamoeba histolytica HM-1:IMSS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9V21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9V21 FirstGlance]. <br>
			</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9v21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9v21 OCA], [https://pdbe.org/9v21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9v21 RCSB], [https://www.ebi.ac.uk/pdbsum/9v21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9v21 ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RL8_ENTH1 RL8_ENTH1] Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.[UniProtKB:P62917]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with poor sanitation conditions, and it remains the fourth leading cause of death due to a protozoan infection. E. histolytica life cycle spans between an infective 'cyst stage' and an active disease-causing 'trophozoite stage'. We have isolated ribosomes from the trophozoite stage of E. histolytica. Here, we report single particle cryo-EM structures of the 53S ribosome large subunit (LSU), and 75S associated ribosomes, with P-tRNA, A/P and P/E tRNAs, and with paromomycin antibiotic, at 2.8 A to 3.4 A resolution. The E. histolytica possesses a reduced ribosome with a conserved core, and the periphery evolved with species-specific unique features. The most notable features are the presence of the rRNA triple helix near the peptide exit tunnel, the expansion segment H88ES102 near the exit site on LSU, and unique insertions in r-proteins. Furthermore, the 75S ribosome paromomycin complex structure provides the atomic details of its interactions. These structures provide insights into the evolutionary adaptation of the E. histolytica translational machinery and may be explored further for amoebicidal therapeutic intervention.

	~~Authors:~~ Sharma, S., Mishra, S., Gourinath, S., Kaushal~~, P~~.S.		Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture.,Sharma S, Mishra S, Gourinath S, Kaushal PS Nat Commun. 2025 Aug 20;16(1):7758. doi: 10.1038/s41467-025-62767-x. PMID:40835601<ref>PMID:40835601</ref>

	~~Description: Cryo~~-~~EM structure of 75S ribosome with P~~-~~tRNA from~~ Entamoeba histolytica		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased~~ Structures]]		</div>
	[[Category: Gourinath, S]]		<div class="pdbe-citations 9v21" style="background-color:#fffaf0;"></div>
	[[Category: ~~Sharma, S~~]]		== References ==
	[[Category: ~~Kaushal, P.~~S]]		<references/>
	[[Category: ~~Mishra,~~ S]]		__TOC__
			</StructureSection>
			[[Category: Entamoeba histolytica HM-1:IMSS]]
			[[Category: Large Structures]]
			[[Category: Gourinath S]]
			[[Category: Kaushal PS]]
			[[Category: Mishra S]]
			[[Category: Sharma S]]

OCA: Protected "9v21" [edit=sysop:move=sysop]

2025-05-28T04:36:51Z

Protected "9v21" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:36, 28 May 2025
(No difference)

OCA: New page: '''Unreleased structure''' The entry 9v21 is ON HOLD Authors: Sharma, S., Mishra, S., Gourinath, S., Kaushal, P.S. Description: Cryo-EM structure of 75S ribosome with P-tRNA from Entam...

2025-05-28T04:36:51Z

New page: '''Unreleased structure''' The entry 9v21 is ON HOLD Authors: Sharma, S., Mishra, S., Gourinath, S., Kaushal, P.S. Description: Cryo-EM structure of 75S ribosome with P-tRNA from Entam...

New page

'''Unreleased structure'''

The entry 9v21 is ON HOLD

Authors: Sharma, S., Mishra, S., Gourinath, S., Kaushal, P.S.

Description: Cryo-EM structure of 75S ribosome with P-tRNA from Entamoeba histolytica
[[Category: Unreleased Structures]]
[[Category: Gourinath, S]]
[[Category: Sharma, S]]
[[Category: Kaushal, P.S]]
[[Category: Mishra, S]]

9v21 - Revision history

OCA at 07:36, 8 October 2025

OCA: Protected "9v21" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 9v21 is ON HOLD Authors: Sharma, S., Mishra, S., Gourinath, S., Kaushal, P.S. Description: Cryo-EM structure of 75S ribosome with P-tRNA from Entam...