Jhanvi Kath at 14:14, 30 November 2025

2025-11-30T14:14:28Z

← Older revision		Revision as of 14:14, 30 November 2025
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	<gallery>		<gallery>
	File: HSA_Overall_Structure.png\|Overall Cryo-EM structure of HSA (8VAC)		File: HSA_Overall_Structure.png\|Overall Cryo-EM structure of HSA (8VAC)
			File: HSA+Salicylic.png\|HSA with salicylic ligand bound
	</gallery>		</gallery>

	== References ==		== References ==
	<references/>		<references/>

Jhanvi Kath at 14:12, 30 November 2025

2025-11-30T14:12:30Z

← Older revision		Revision as of 14:12, 30 November 2025
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	== Uploaded Figures ==		== Uploaded Figures ==
	~~Insert your PNGs here after uploading (instructions below):~~

	<gallery>		<gallery>
	File:~~YourImage1~~.png\|Cryo-EM ~~map overview~~		File: HSA_Overall_Structure.png\|Overall Cryo-EM structure of HSA (8VAC)
	~~File:YourImage2.png\|Ligand-binding pockets highlighted~~
	</gallery>		</gallery>

	== References ==		== References ==
	<references/>		<references/>

Jhanvi Kath: New page: = Cryo-EM Structure of Human Serum Albumin (2024) = ''By JHANVI'' == Reference Study == Catalano C., Lucier K. W., To D., Senko S., Tran N. L., Farwell A. C., Silva S. M., Dip P. V., Powe...

2025-11-30T14:05:16Z

New page: = Cryo-EM Structure of Human Serum Albumin (2024) = ''By JHANVI'' == Reference Study == Catalano C., Lucier K. W., To D., Senko S., Tran N. L., Farwell A. C., Silva S. M., Dip P. V., Powe...

New page

= Cryo-EM Structure of Human Serum Albumin (2024) =
''By JHANVI''

== Reference Study ==
Catalano C., Lucier K. W., To D., Senko S., Tran N. L., Farwell A. C., Silva S. M., Dip P. V., Poweleit N., Scapin G. (2024).
''The CryoEM structure of human serum albumin in complex with ligands.''
Journal of Structural Biology, 216(3):108105.
DOI: 10.1016/j.jsb.2024.108105

PDB ID: **8VAC**

== Structure ==
<StructureSection load='8VAC' size='340' side='right' caption='Cryo-EM structure of human serum albumin bound to ligands (8VAC, 2024)' scene=''>
Human Serum Albumin (HSA) is the dominant plasma protein responsible for transport, buffering, and molecular trafficking.
The 2024 cryo-EM map (8VAC) captures HSA in complex with multiple ligands under near-physiological biochemical conditions, revealing:

* Three-domain organization (I, II, III), each with A/B subdomains.
* A flexible, heart-shaped tertiary fold.
* Multiple hydrophobic binding pockets for drugs, fatty acids, and endogenous molecules.
* Conformational adjustments in Sudlow sites I and II upon ligand engagement.
* Domain movements consistent with adaptive ligand accommodation.

The loaded 8VAC structure provides a solution-like, high-fidelity representation of the molecule.
</StructureSection>

== Function ==
HSA acts as a systemic transport hub in the bloodstream.
Major physiological roles include:

* Binding and transport of **fatty acids**, **bilirubin**, hormones, and xenobiotics.
* Strong influence on **pharmacokinetics** for albumin-bound drugs.
* Maintenance of **colloid osmotic pressure**.
* Participation in **pH buffering** and redox balance.

The 8VAC cryo-EM structure highlights how the protein repositions domains to optimize ligand engagement.

== Disease ==
Structural or concentration changes in HSA correlate with many pathologies:

* **Hypoalbuminemia** in liver disease, sepsis, nephrotic syndrome.
* **Glycation and oxidation** in diabetes or chronic kidney disease, altering ligand affinity.
* **Hereditary albumin variants**, impacting stability and drug-binding profiles.
* Altered levels in **inflammation**, **cancer**, and severe infection influence drug dosing outcomes.

Structural mapping enables correlation of clinical conditions with ligand-pocket geometry.

== Relevance ==
The 8VAC structure is directly applicable to:

* **Drug design** – understanding pocket architecture assists affinity and specificity tuning.
* **PK/PD modeling** – high-affinity albumin binders require dosage fine-tuning.
* **Albumin-based drug delivery systems** – nanoparticles and small molecules utilize albumin’s long circulation time.
* **Biomarker interpretation** – albumin concentration is essential in diagnoses and scoring systems.

This structure bridges clinical context and molecular understanding in a single cryo-EM model.

== Structural Highlights ==
Below are placeholder SAT scenes that you can replace with your own:

* Overall 3-domain organization:
<scene name="/sandbox/jhanvi/HSA_8VAC/overall/1">Domain overview</scene>

* Sudlow Site I (Domain IIA):
<scene name="/sandbox/jhanvi/HSA_8VAC/siteI/1">Primary drug-binding pocket</scene>

* Sudlow Site II (Domain IIIA):
<scene name="/sandbox/jhanvi/HSA_8VAC/siteII/1">Secondary drug-binding pocket</scene>

* Flexibility + domain movements:
<scene name="/sandbox/jhanvi/HSA_8VAC/flexibility/1">Hinge flexibility</scene>

== Uploaded Figures ==
Insert your PNGs here after uploading (instructions below):

<gallery>
File:YourImage1.png|Cryo-EM map overview
File:YourImage2.png|Ligand-binding pockets highlighted
</gallery>

== References ==
<references/>

CreateSandbox - Revision history

Jhanvi Kath at 14:14, 30 November 2025

Jhanvi Kath at 14:12, 30 November 2025

Jhanvi Kath: New page: = Cryo-EM Structure of Human Serum Albumin (2024) = ''By JHANVI'' == Reference Study == Catalano C., Lucier K. W., To D., Senko S., Tran N. L., Farwell A. C., Silva S. M., Dip P. V., Powe...