Intersectin - Revision history

Michal Harel at 08:32, 5 October 2025

2025-10-05T08:32:56Z

← Older revision		Revision as of 08:32, 5 October 2025
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	*Intersectin 1 long isoform		*Intersectin 1 long isoform

	**[[~~1ki1~~]] - hITSN1 DBL+PH domains 1229-1580 + Cdc42<br />		**[[5hzj]] - hITSN1 DBL+PH domains 1229-1580 <br />
			**[[5hzi]] - hITSN1 DBL+PH domains (mutant)<br />
			**[[1ki1]], [[5hzk]] - hITSN1 DBL+PH domains + Cdc42<br />
	**[[3qbv]] - hITSN1 DBL+PH domains (mutant) + Cdc42<br />		**[[3qbv]] - hITSN1 DBL+PH domains (mutant) + Cdc42<br />
	**[[3jv3]] - mITSN1 5th SH3+DH domains 1151-1431 <br />		**[[3jv3]] - mITSN1 5th SH3+DH domains 1151-1431 <br />

Michal Harel at 11:51, 7 July 2024

2024-07-07T11:51:05Z

← Older revision		Revision as of 11:51, 7 July 2024
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	== Function ==		== Function ==

	'''Intersectin''' (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains<ref>PMID:29229830</ref>. Alternative splicing of ITSN generates a short isoform with 2 EH (Eps15 homology) domains and 5 SH3 domains and a long isoform with additional DH, PH and C2 domains<ref>PMID:19037327</ref>,<ref>PMID:10922467</ref>.		'''Intersectin''' (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains<ref>PMID:29229830</ref>.

	'''ITSN 2''' is also involved in clathrin-mediated endocytosis and is very similar to '''ITSN 1'''.		'''ITSN 2''' is also involved in clathrin-mediated endocytosis and is very similar to '''ITSN 1'''.

Michal Harel at 11:49, 7 July 2024

2024-07-07T11:49:25Z

← Older revision		Revision as of 11:49, 7 July 2024
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	*'''ITSN-1 short form''' is 140kD and expressed in all tissues		*'''ITSN-1 short form''' is 140kD and expressed in all tissues
	*'''ITSN-1 long form''' is 200kD and expressed in neutrons, eye, heart, blood cells and kidney<ref>PMID:32161523</ref>.		*'''ITSN-1 long form''' is 200kD and expressed in neutrons, eye, heart, blood cells and kidney<ref>PMID:32161523</ref>.
			*'''ITSN-2 short form''' contains 2 EH domains, a coiled-coil domain and 5 SH3 domains
			*'''ITSN-2 long form'''contains an extra carboxylate domain<ref>PMID:10922467</ref>.

	== Relevance ==		== Relevance ==

Michal Harel at 11:43, 7 July 2024

2024-07-07T11:43:30Z

← Older revision		Revision as of 11:43, 7 July 2024
Line 5:		Line 5:
	'''Intersectin''' (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains<ref>PMID:29229830</ref>. Alternative splicing of ITSN generates a short isoform with 2 EH (Eps15 homology) domains and 5 SH3 domains and a long isoform with additional DH, PH and C2 domains<ref>PMID:19037327</ref>,<ref>PMID:10922467</ref>.		'''Intersectin''' (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains<ref>PMID:29229830</ref>. Alternative splicing of ITSN generates a short isoform with 2 EH (Eps15 homology) domains and 5 SH3 domains and a long isoform with additional DH, PH and C2 domains<ref>PMID:19037327</ref>,<ref>PMID:10922467</ref>.

	'''ITSN 2''' is also involved in clathrin-mediated endocytosis and is very similar to '''ITSN 1'''.		'''ITSN 2''' is also involved in clathrin-mediated endocytosis and is very similar to '''ITSN 1'''.

			ITSN-1 has two isoforms as a result of differential splicing:
			*'''ITSN-1 short form''' is 140kD and expressed in all tissues
			*'''ITSN-1 long form''' is 200kD and expressed in neutrons, eye, heart, blood cells and kidney<ref>PMID:32161523</ref>.


	== Relevance ==		== Relevance ==

Alexander Berchansky at 17:22, 18 January 2021

2021-01-18T17:22:15Z

← Older revision		Revision as of 17:22, 18 January 2021
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	== Structural highlights ==		== Structural highlights ==

	The 3D structure of <scene name='84/842982/Cv/3'>the complex between the SH3 domains of ITSN 1 and FCHSD2</scene> shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.		The 3D structure of <scene name='84/842982/Cv/5'>the complex between the SH3 domains of ITSN 1 and FCHSD2</scene> shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 14:14, 4 May 2020

2020-05-04T14:14:45Z

← Older revision		Revision as of 14:14, 4 May 2020
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	<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (~~grey~~, pink, light pink, orange) (PDB code [[6gbu]])' scene='84/842982/Cv/1'>		<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (deepskyblue, pink, light pink, orange) (PDB code [[6gbu]])' scene='84/842982/Cv/4'>

	== Function ==		== Function ==
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	== Structural highlights ==		== Structural highlights ==

	The 3D structure of <scene name='84/842982/Cv/2'>the complex between the SH3 domains of ITSN 1 and FCHSD2</scene> shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.		The 3D structure of <scene name='84/842982/Cv/3'>the complex between the SH3 domains of ITSN 1 and FCHSD2</scene> shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 14:07, 4 May 2020

2020-05-04T14:07:17Z

← Older revision		Revision as of 14:07, 4 May 2020
Line 15:		Line 15:
	== Structural highlights ==		== Structural highlights ==

	The 3D structure of the complex between the SH3 domains of ITSN 1 and FCHSD2 shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.		The 3D structure of <scene name='84/842982/Cv/2'>the complex between the SH3 domains of ITSN 1 and FCHSD2</scene> shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.

	</StructureSection>		</StructureSection>

Alexander Berchansky at 13:56, 4 May 2020

2020-05-04T13:56:12Z

← Older revision		Revision as of 13:56, 4 May 2020
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	<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code [[6gbu]])' scene=''>		<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code [[6gbu]])' scene='84/842982/Cv/1'>

	== Function ==		== Function ==

Michal Harel at 09:51, 29 April 2020

2020-04-29T09:51:19Z

← Older revision		Revision as of 09:51, 29 April 2020
Line 1:		Line 1:
	<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code [[6gbu]])' scene=''>		<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 short isoform 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code [[6gbu]])' scene=''>

	== Function ==		== Function ==

Michal Harel at 09:50, 29 April 2020

2020-04-29T09:50:44Z

← Older revision		Revision as of 09:50, 29 April 2020
Line 24:		Line 24:
	{{#tree:id=OrganizedByTopic\|openlevels=0\|		{{#tree:id=OrganizedByTopic\|openlevels=0\|

	*Intersectin 1		*Intersectin 1 short isoform

	**[[3fia]] - hITSN1 EH domain 1-111<br />		**[[3fia]] - hITSN1 EH domain 1-111<br />
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	**[[4iim]] - hITSN1 2nd SH3 domain + peptide<br />		**[[4iim]] - hITSN1 2nd SH3 domain + peptide<br />
	**[[6gbu]] - hITSN1 4th SH3 domain 1055-1133 + FCHSD2<br />		**[[6gbu]] - hITSN1 4th SH3 domain 1055-1133 + FCHSD2<br />

			*Intersectin 1 long isoform

	**[[1ki1]] - hITSN1 DBL+PH domains 1229-1580 + Cdc42<br />		**[[1ki1]] - hITSN1 DBL+PH domains 1229-1580 + Cdc42<br />
	**[[3qbv]] - hITSN1 DBL+PH domains (mutant) + Cdc42<br />		**[[3qbv]] - hITSN1 DBL+PH domains (mutant) + Cdc42<br />
	**[[3jv3]] - mITSN1 5th SH3+DH domains 1151-1431 <br />		**[[3jv3]] - mITSN1 5th SH3+DH domains 1151-1431 <br />

	*Intersectin 2		*Intersectin 2 short isoform

	**[[1uff]] - hITSN2 1st SH3 domain 761-841 - NMR<br />		**[[1uff]] - hITSN2 1st SH3 domain 761-841 - NMR<br />
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	**[[1ue9]] - hITSN2 4th SH3 domain 1055-1121 - NMR<br />		**[[1ue9]] - hITSN2 4th SH3 domain 1055-1121 - NMR<br />
	**[[1udl]] - hITSN2 5th SH3 domain 1102-1186 - NMR<br />		**[[1udl]] - hITSN2 5th SH3 domain 1102-1186 - NMR<br />

			*Intersectin 2 long isoform

	**[[3gf9]] - hITSN2 RhoGEF domain 1102-1378 <br />		**[[3gf9]] - hITSN2 RhoGEF domain 1102-1378 <br />
	**[[3jzy]] - hITSN2 C2 domain 1173-1664 <br />		**[[3jzy]] - hITSN2 C2 domain 1173-1664 <br />