Matriptase - Revision history

Michal Harel at 10:42, 28 October 2019

2019-10-28T10:42:19Z

← Older revision		Revision as of 10:42, 28 October 2019
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	</StructureSection>		</StructureSection>

	~~== 3D Structures of matriptase ==~~

	~~Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~
	~~{{#tree:id=OrganizedByTopic\|openlevels=0\|~~

	*'''matriptase'''

	**[[4is5]], [[5lyo]] – hST14 catalytic domain - human<br />

	*Matriptase complex with small molecule inhibitor

	**[[1eax]] – hST14 catalytic domain + benzamidine <br />
	**[[3p8g]] – hST14 residues 182-422 (mutant) + benzamidine <br />
	**[[2gv6]] – hST14 catalytic domain + benzamidine derivative<br />
	**[[4o97]], [[4o9v]] – hST14 catalytic domain + benzamidine derivative + tetrapeptide<br />
	**[[3ncl]] – hST14 catalytic domain (mutant) + benzamidine derivative<br />
	**[[2gv7]], [[4jz1]], [[4jzi]], [[4jyt]], [[4r0i]] – hST14 catalytic domain + inhibitor<br />
	**[[6n4t]] – hST14 catalytic domain + benzothiazole derivative<br />

	*Matriptase complex with polypeptide inhibitor

	**[[1eaw]] – hST14 catalytic domain + BPTI<br />
	**[[3bn9]] – hST14 catalytic domain + antibody<br />
	**[[3nps]], [[3so3]] – hST14 catalytic domain (mutant) + antibody<br />
	**[[3p8f]] – hST14 residues 182-422 (mutant) + trypsin inhibitor peptide <br />
	**[[4isl]], [[4isn]], [[4iso]] – hST14 catalytic domain (mutant) + Kunitz-type protease inhibitor <br />
	}}

	== References ==		== References ==

Michal Harel at 10:41, 28 October 2019

2019-10-28T10:41:18Z

← Older revision		Revision as of 10:41, 28 October 2019
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	== Structural highlights ==		== Structural highlights ==
	ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the <scene name='59/595760/Cv/5'>Ser-His-Asp catalytic triad</scene> and <scene name='59/595760/Cv/6'>Gly-Ser oxyanion hole</scene>. The <scene name='59/595760/Cv/7'>inhibitor benzamidine blocks the catalytic triad</scene><ref>PMID:11696548</ref>. Water molecules shown as red spheres.		ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the <scene name='59/595760/Cv/5'>Ser-His-Asp catalytic triad</scene> and <scene name='59/595760/Cv/6'>Gly-Ser oxyanion hole</scene>. The <scene name='59/595760/Cv/7'>inhibitor benzamidine blocks the catalytic triad</scene><ref>PMID:11696548</ref>. Water molecules shown as red spheres.

			== 3D Structures of matriptase ==
			[[Matriptase 3D structures]]

	</StructureSection>		</StructureSection>

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	*'''matriptase'''		*'''matriptase'''

	**[[4is5]] – hST14 catalytic domain - human<br />		**[[4is5]], [[5lyo]] – hST14 catalytic domain - human<br />

	*Matriptase complex with small molecule inhibitor		*Matriptase complex with small molecule inhibitor
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	**[[4o97]], [[4o9v]] – hST14 catalytic domain + benzamidine derivative + tetrapeptide<br />		**[[4o97]], [[4o9v]] – hST14 catalytic domain + benzamidine derivative + tetrapeptide<br />
	**[[3ncl]] – hST14 catalytic domain (mutant) + benzamidine derivative<br />		**[[3ncl]] – hST14 catalytic domain (mutant) + benzamidine derivative<br />
	**[[2gv7]], [[4jz1]], [[4jzi]], [[4jyt]] – hST14 catalytic domain + inhibitor<br />		**[[2gv7]], [[4jz1]], [[4jzi]], [[4jyt]], [[4r0i]] – hST14 catalytic domain + inhibitor<br />
			**[[6n4t]] – hST14 catalytic domain + benzothiazole derivative<br />

	*Matriptase complex with polypeptide inhibitor		*Matriptase complex with polypeptide inhibitor

Alexander Berchansky at 09:47, 10 July 2019

2019-07-10T09:47:44Z

← Older revision		Revision as of 09:47, 10 July 2019
Line 10:		Line 10:

	== Structural highlights ==		== Structural highlights ==
	ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the <scene name='59/595760/Cv/2'>Ser-His-Asp catalytic triad</scene> and <scene name='59/595760/Cv/3'>Gly-Ser oxyanion hole</scene>. The <scene name='59/595760/Cv/4'>inhibitor benzamidine blocks the catalytic triad</scene><ref>PMID:11696548</ref>. Water molecules shown as red spheres.		ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the <scene name='59/595760/Cv/5'>Ser-His-Asp catalytic triad</scene> and <scene name='59/595760/Cv/6'>Gly-Ser oxyanion hole</scene>. The <scene name='59/595760/Cv/7'>inhibitor benzamidine blocks the catalytic triad</scene><ref>PMID:11696548</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>

Alexander Berchansky at 08:19, 25 April 2016

2016-04-25T08:19:49Z

← Older revision		Revision as of 08:19, 25 April 2016
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	<StructureSection load='1eax' size='350' side='right' caption='Structure of human matriptase catalytic domain ~~(grey)~~ complex with benzamidine and sulfate (PDB code [[1eax]]). ' scene='59/595760/Cv/1'>		<StructureSection load='1eax' size='350' side='right' caption='Structure of human matriptase catalytic domain complex with benzamidine and sulfate (PDB code [[1eax]]). ' scene='59/595760/Cv/1'>

	== Function ==		== Function ==
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	== Structural highlights ==		== Structural highlights ==
	ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the Ser-His-Asp catalytic triad and Gly-Ser oxyanion hole. The inhibitor benzamidine blocks the catalytic triad<ref>PMID:11696548</ref>.		ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the <scene name='59/595760/Cv/2'>Ser-His-Asp catalytic triad</scene> and <scene name='59/595760/Cv/3'>Gly-Ser oxyanion hole</scene>. The <scene name='59/595760/Cv/4'>inhibitor benzamidine blocks the catalytic triad</scene><ref>PMID:11696548</ref>. Water molecules shown as red spheres.
	</StructureSection>		</StructureSection>

Alexander Berchansky at 08:02, 25 April 2016

2016-04-25T08:02:18Z

← Older revision		Revision as of 08:02, 25 April 2016
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	<StructureSection load='1eax' size='~~340~~' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with benzamidine and sulfate (PDB code [[1eax]]). ' scene=''>		<StructureSection load='1eax' size='350' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with benzamidine and sulfate (PDB code [[1eax]]). ' scene='59/595760/Cv/1'>

	== Function ==		== Function ==

Michal Harel at 07:44, 19 April 2016

2016-04-19T07:44:23Z

← Older revision		Revision as of 07:44, 19 April 2016
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	== Function ==		== Function ==

	'''Matriptase''' or '''suppressor of tumorigenicity 14 protein''' (ST14) is an epithelial-derived, membrane multi-domain serine protease. ST14 cleaves and activates hepatocyte ~~frowth~~ factor/scatter factor and urokinase plasminogen. Benzamidine is an inhibitor of ST14. ST14 catalytic domain contains residues 615-855.		'''Matriptase''' or '''suppressor of tumorigenicity 14 protein''' (ST14) is an epithelial-derived, membrane multi-domain serine protease. ST14 cleaves and activates hepatocyte growth factor/scatter factor and urokinase plasminogen. Benzamidine is an inhibitor of ST14. ST14 catalytic domain contains residues 615-855<ref>PMID:16838070</ref>.

	~~== Disease ==~~

	== Relevance ==		== Relevance ==

	ST14 is a target for anti-cancer therapy.		ST14 has a role in ovarian cancer and is a target for anti-cancer therapy<ref>PMID:12779007</ref>. Inflammation-associated reactive oxygen species and tissue acidity enhance ST14 activation in some skin diseases<ref>PMID:21123732</ref>.

	== Structural highlights ==		== Structural highlights ==
	ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the Ser-His-Asp catalytic triad and Gly-Ser oxyanion hole. The inhibitor benzamidine blocks the ~~cataytic~~ triad<ref>PMID:11696548</ref>.		ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the Ser-His-Asp catalytic triad and Gly-Ser oxyanion hole. The inhibitor benzamidine blocks the catalytic triad<ref>PMID:11696548</ref>.
	</StructureSection>		</StructureSection>

Michal Harel at 07:01, 19 April 2016

2016-04-19T07:01:11Z

← Older revision		Revision as of 07:01, 19 April 2016
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	<StructureSection load='1eax' size='340' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with ~~Kunitz-type protease inhibitor (green)~~ and ~~glutathione~~ (PDB code [[~~4isn~~]]). ' scene=''>		<StructureSection load='1eax' size='340' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with benzamidine and sulfate (PDB code [[1eax]]). ' scene=''>

	== Function ==		== Function ==

Michal Harel at 06:59, 19 April 2016

2016-04-19T06:59:38Z

← Older revision		Revision as of 06:59, 19 April 2016
Line 1:		Line 1:
	<StructureSection load='~~4isn~~' size='340' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with Kunitz-type protease inhibitor (green) and glutathione (PDB code [[4isn]]). ' scene=''>		<StructureSection load='1eax' size='340' side='right' caption='Structure of human matriptase catalytic domain (grey) complex with Kunitz-type protease inhibitor (green) and glutathione (PDB code [[4isn]]). ' scene=''>

	== Function ==		== Function ==
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	== Structural highlights ==		== Structural highlights ==
			ST14 active site has the conformation of a typical serine protease like trypsin or chymotrypsin with the Ser-His-Asp catalytic triad and Gly-Ser oxyanion hole. The inhibitor benzamidine blocks the cataytic triad<ref>PMID:11696548</ref>.
	</StructureSection>		</StructureSection>

Michal Harel at 08:02, 1 December 2014

2014-12-01T08:02:32Z

← Older revision		Revision as of 08:02, 1 December 2014
Line 12:		Line 12:

	== Structural highlights ==		== Structural highlights ==
			</StructureSection>

	== 3D Structures of matriptase ==		== 3D Structures of matriptase ==

	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
			{{#tree:id=OrganizedByTopic\|openlevels=0\|

	~~===~~matriptase~~===~~		*'''matriptase'''

	[[4is5]] – hST14 catalytic domain - human<br />		**[[4is5]] – hST14 catalytic domain - human<br />

	~~===~~Matriptase complex with small molecule inhibitor~~===~~		*Matriptase complex with small molecule inhibitor

	[[1eax]] – hST14 catalytic domain + benzamidine <br />		**[[1eax]] – hST14 catalytic domain + benzamidine <br />
	[[3p8g]] – hST14 residues 182-422 (mutant) + benzamidine <br />		**[[3p8g]] – hST14 residues 182-422 (mutant) + benzamidine <br />
	[[2gv6]] – hST14 catalytic domain + benzamidine derivative<br />		**[[2gv6]] – hST14 catalytic domain + benzamidine derivative<br />
	[[4o97]], [[4o9v]] – hST14 catalytic domain + benzamidine derivative + tetrapeptide<br />		**[[4o97]], [[4o9v]] – hST14 catalytic domain + benzamidine derivative + tetrapeptide<br />
	[[3ncl]] – hST14 catalytic domain (mutant) + benzamidine derivative<br />		**[[3ncl]] – hST14 catalytic domain (mutant) + benzamidine derivative<br />
	[[2gv7]], [[4jz1]], [[4jzi]], [[4jyt]] – hST14 catalytic domain + inhibitor<br />		**[[2gv7]], [[4jz1]], [[4jzi]], [[4jyt]] – hST14 catalytic domain + inhibitor<br />

	~~===~~Matriptase complex with polypeptide inhibitor~~===~~		*Matriptase complex with polypeptide inhibitor

	~~[[1eaw]] – hST14 catalytic domain + BPTI<br />~~
	~~[[3bn9]] – hST14 catalytic domain + antibody<br />~~
	~~[[3nps]], [[3so3]] – hST14 catalytic domain (mutant) + antibody<br />~~
	~~[[3p8f]] – hST14 residues 182-422 (mutant) + trypsin inhibitor peptide <br />~~
	~~[[4isl]], [[4isn]], [[4iso]] – hST14 catalytic domain (mutant) + Kunitz-type protease inhibitor <br />~~

			**[[1eaw]] – hST14 catalytic domain + BPTI<br />
			**[[3bn9]] – hST14 catalytic domain + antibody<br />
			**[[3nps]], [[3so3]] – hST14 catalytic domain (mutant) + antibody<br />
			**[[3p8f]] – hST14 residues 182-422 (mutant) + trypsin inhibitor peptide <br />
			**[[4isl]], [[4isn]], [[4iso]] – hST14 catalytic domain (mutant) + Kunitz-type protease inhibitor <br />
			}}

	== References ==		== References ==

Michal Harel at 06:28, 2 September 2014

2014-09-02T06:28:26Z

← Older revision		Revision as of 06:28, 2 September 2014
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	Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}		Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

			===matriptase===

	[[4is5]] – hST14 catalytic domain - human<br />		[[4is5]] – hST14 catalytic domain - human<br />