Net charge - Revision history

Eric Martz: /* Calculating Net Charge Using Protein Calculator */

2024-10-26T19:24:39Z

Calculating Net Charge Using Protein Calculator

← Older revision		Revision as of 19:24, 26 October 2024
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	<br>You can calculate the net charge of your protein easily at a given pH or over a range:		<br>You can calculate the net charge of your protein easily at a given pH or over a range:

	==~~Calculating Net Charge Using~~ Protein Calculator==		==Protein Calculator==
	#First, get the one-letter amino acid sequence of your protein of interest.		#First, get the one-letter amino acid sequence of your protein of interest.
	##See the [[Isoelectric point\|Isoelectric point page]] for some methods.		##See the [[Isoelectric point\|Isoelectric point page]] for some methods.
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	#Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator].		#Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator].
	#Check the first box under Charge at the right, and click Submit Query.		#Check the first box under Charge at the right, and click Submit Query.

			==Prot-pi==
			"Prot pi \| Protein Tool calculates isoelectric point and net charge of proteins, as well as the exact mass and the absorption coefficient using the amino acid sequence."

			*Go to [https://www.protpi.ch/Calculator/ProteinTool Prot-pi]] and follow instructions.

	==See Also==		==See Also==

Eric Martz: /* Calculating Net Charge Using Protein Calculator */

2013-05-15T02:59:27Z

Calculating Net Charge Using Protein Calculator

← Older revision		Revision as of 02:59, 15 May 2013
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	### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).		### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).
	###Click on the link for the one-letter amino acid sequence for one chain.		###Click on the link for the one-letter amino acid sequence for one chain.
	#Copy the sequence and paste it into the large box at Christopher Putnam's [http://~~www~~.~~scripps~~.~~edu/~cdputnam~~/~~protcalc.html~~ Protein Calculator].		#Copy the sequence and paste it into the large box at Christopher Putnam's [http://protcalc.sourceforge.net/ Protein Calculator].
	#Check the first box under Charge at the right, and click Submit Query.		#Check the first box under Charge at the right, and click Submit Query.

Eric Martz: /* Calculating Net Charge Using Protein Calculator */

2011-10-19T18:41:18Z

Calculating Net Charge Using Protein Calculator

← Older revision		Revision as of 18:41, 19 October 2011
Line 5:		Line 5:
	#First, get the one-letter amino acid sequence of your protein of interest.		#First, get the one-letter amino acid sequence of your protein of interest.
	##See the [[Isoelectric point\|Isoelectric point page]] for some methods.		##See the [[Isoelectric point\|Isoelectric point page]] for some methods.
	##Or for a protein that has a ~~solved~~ in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.		##Or for a protein that has a 3D structure in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.
	### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).		### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).
	###Click on the link for the one-letter amino acid sequence for one chain.		###Click on the link for the one-letter amino acid sequence for one chain.

Wayne Decatur at 18:26, 17 January 2011

2011-01-17T18:26:00Z

← Older revision		Revision as of 18:26, 17 January 2011
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	==Content Attribution==		==Content Attribution==
	The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz].		The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz].
			[[Category:Topic Page]]

Wayne Decatur at 18:38, 21 November 2010

2010-11-21T18:38:53Z

← Older revision		Revision as of 18:38, 21 November 2010
Line 4:		Line 4:
	==Calculating Net Charge Using Protein Calculator==		==Calculating Net Charge Using Protein Calculator==
	#First, get the one-letter amino acid sequence of your protein of interest.		#First, get the one-letter amino acid sequence of your protein of interest.
	##See the [[~~Isolectric~~ point\|~~Isolectric~~ point page]] for some methods.		##See the [[Isoelectric point\|Isoelectric point page]] for some methods.
	##Or for a protein that has a solved in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.		##Or for a protein that has a solved in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.
	### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).		### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).

Wayne Decatur at 18:38, 21 November 2010

2010-11-21T18:38:18Z

← Older revision		Revision as of 18:38, 21 November 2010
Line 3:		Line 3:

	==Calculating Net Charge Using Protein Calculator==		==Calculating Net Charge Using Protein Calculator==
	#In Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.		#First, get the one-letter amino acid sequence of your protein of interest.
	# At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).		##See the [[Isolectric point\|Isolectric point page]] for some methods.
	#Click on the link for the one-letter amino acid sequence for one chain.		##Or for a protein that has a solved in Proteopedia, find the page for your structure named for PDB file code and click on [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA] under the structure.
			### At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom).
			###Click on the link for the one-letter amino acid sequence for one chain.
	#Copy the sequence and paste it into the large box at Christopher Putnam's [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator].		#Copy the sequence and paste it into the large box at Christopher Putnam's [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator].
	#~~Choose your options in or near~~ the ~~second or third check boxes~~ box under Charge at the right, and click Submit Query.		#Check the first box under Charge at the right, and click Submit Query.

	==See Also==		==See Also==

Wayne Decatur: fix a typo and a link

2010-11-21T18:05:48Z

fix a typo and a link

← Older revision		Revision as of 18:05, 21 November 2010
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	In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the ~~constiuent~~ amino acids. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge.		In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the constituent amino acids. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge.
	<br>You can calculate the net charge of your protein easily at a given pH or over a range:		<br>You can calculate the net charge of your protein easily at a given pH or over a range:

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	==See Also==		==See Also==
	*[[Isoelectric point]]		*[[Isoelectric point]]
	*[[Monovalent Cation Binding in Cubic Insulin Crystals~~\|9ins~~]]		*[[9ins\|Monovalent Cation Binding in Cubic Insulin Crystals]]

	==Content Attribution==		==Content Attribution==
	The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz].		The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz].

Wayne Decatur at 18:04, 21 November 2010

2010-11-21T18:04:59Z

← Older revision		Revision as of 18:04, 21 November 2010
Line 11:		Line 11:
	==See Also==		==See Also==
	*[[Isoelectric point]]		*[[Isoelectric point]]
	*[[9ins]]		*[[Monovalent Cation Binding in Cubic Insulin Crystals\|9ins]]

	==Content Attribution==		==Content Attribution==
	The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class ~~sylabus~~ taught by Eric Martz].		The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class syllabus taught by Eric Martz].

Wayne Decatur: /* See Also */

2009-10-27T18:49:16Z

Wayne Decatur at 20:55, 25 October 2009

2009-10-25T20:55:40Z

← Older revision		Revision as of 20:55, 25 October 2009
Line 1:		Line 1:
	In a solution of a given pH, a protein will have a negative or positive net charge. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge.		In a solution of a given pH, a protein will have a negative or positive net charge dependent on the pKa values of the acidic and basic groups of the constiuent amino acids. The [[isoelectric point]], or pI, is the pH at which a protein has zero net charge. When the pH is higher than the isoelectric point, the protein has negative net charge, and when lower, positive net charge.
	<br>You can calculate the net charge of your protein easily at a given pH or over a range:		<br>You can calculate the net charge of your protein easily at a given pH or over a range:

← Older revision		Revision as of 18:49, 27 October 2009
Line 11:		Line 11:
	==See Also==		==See Also==
	*[[Isoelectric point]]		*[[Isoelectric point]]
			*[[9ins]]

	==Content Attribution==		==Content Attribution==
	The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class sylabus taught by Eric Martz].		The calculation method was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class sylabus taught by Eric Martz].