Pre-mRNA-splicing factor - Revision history

Michal Harel at 09:17, 27 November 2019

2019-11-27T09:17:58Z

Michal Harel at 09:16, 27 November 2019

2019-11-27T09:16:59Z

← Older revision		Revision as of 09:16, 27 November 2019
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	**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />		**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
	**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />		**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />
			**[[6nqi]] – Prp RH domain – red alga <br />
	**[[2p8r]] – CePrp C terminal (mutant) – ''Caenorhabditis elegans''<br />		**[[2p8r]] – CePrp C terminal (mutant) – ''Caenorhabditis elegans''<br />
	**[[2p87]] – CePrp C terminal <br />		**[[2p87]] – CePrp C terminal <br />
			**[[6mwy]] – Prp – ''Cryptococcus gattii'' <br />

	*Pre-mRNA-splicing factor 9		*Pre-mRNA-splicing factor 9
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	*Pre-mRNA-splicing factors in spliceosome		*Pre-mRNA-splicing factors in spliceosome

	**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />		**[[3jcr]], [[5o9z]], [[6id1]], [[6id0]], [[6icz]], [[6ff7]], [[6ff4]], [[6ah0]], [[5z58]] – hPrp - CryoEM<br />
	**[[3jb9]] – yPrp <br />		**[[3jb9]] – yPrp <br />
	**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]], [[6g90]], [[6exn]], [[6bk8]], [[5ylz]], [[5y88]] – yPrp - CryoEM<br />		**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]], [[6g90]], [[6exn]], [[6bk8]], [[5ylz]], [[5y88]], [[6j6q]], [[6j6n]], [[6j6h]], [[6j6g]], [[5zwo]] – yPrp - CryoEM<br />

	*Pre-mRNA-splicing factor cwc2		*Pre-mRNA-splicing factor cwc2

Michal Harel at 08:56, 27 November 2019

2019-11-27T08:56:15Z

← Older revision		Revision as of 08:56, 27 November 2019
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	== Structural highlights ==		== Structural highlights ==
	<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.		<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.

			== 3D Structures of pre-mRNA splicing factors ==
			[[Pre-mRNA splicing factors 3D structures]]

	</StructureSection>		</StructureSection>

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	*Pre-mRNA-splicing factor 8		*Pre-mRNA-splicing factor 8

			**[[3lru]] – hPrp residues 1831-1990<br />
			**[[3e9l]], [[3enb]] – hPrp endonuclease domain<br />
	**[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 <br />		**[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 <br />
	**[[4jk7]], [[4jk9]], [[3e9o]], [[3e9p]], [[3e66]] - yPrp RNA-binding domain <br />		**[[4jk7]], [[4jk9]], [[3e9o]], [[3e9p]], [[3e66]] - yPrp RNA-binding domain <br />
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	**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />		**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
	**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />		**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />
	**[[3lru]] – hPrp residues 1831-1990<br />
	**[[3e9l]], [[3enb]] – hPrp endonuclease domain<br />
	**[[2p8r]] – CePrp C terminal (mutant) – ''Caenorhabditis elegans''<br />		**[[2p8r]] – CePrp C terminal (mutant) – ''Caenorhabditis elegans''<br />
	**[[2p87]] – CePrp C terminal <br />		**[[2p87]] – CePrp C terminal <br />
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	*Pre-mRNA-splicing factor 20		*Pre-mRNA-splicing factor 20

			**[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
	**[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />		**[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
	**[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />

	*Pre-mRNA-splicing factor 21		*Pre-mRNA-splicing factor 21
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	*Pre-mRNA-splicing factors in spliceosome		*Pre-mRNA-splicing factors in spliceosome

			**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />
	**[[3jb9]] – yPrp <br />		**[[3jb9]] – yPrp <br />
	**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]], [[6g90]], [[6exn]], [[6bk8]], [[5ylz]], [[5y88]] – yPrp - CryoEM<br />		**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]], [[6g90]], [[6exn]], [[6bk8]], [[5ylz]], [[5y88]] – yPrp - CryoEM<br />
	**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />

	*Pre-mRNA-splicing factor cwc2		*Pre-mRNA-splicing factor cwc2

Alexander Berchansky at 13:41, 12 August 2019

2019-08-12T13:41:50Z

← Older revision		Revision as of 13:41, 12 August 2019
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	== Structural highlights ==		== Structural highlights ==
	<scene name='46/462200/Cv/2'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.		<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.
	</StructureSection>		</StructureSection>

Michal Harel at 10:07, 7 February 2019

2019-02-07T10:07:41Z

← Older revision		Revision as of 10:07, 7 February 2019
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	*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />		*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />
	*For '''Prp19''' see [[Prp19]]<br />		*For '''Prp19''' see [[Prp19]]<br />
			*For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br />
	*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />		*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />
	*For '''Prp40''' see [[Prp40]].<br />		*For '''Prp40''' see [[Prp40]].<br />

Michal Harel at 09:52, 26 August 2018

2018-08-26T09:52:53Z

← Older revision		Revision as of 09:52, 26 August 2018
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	<StructureSection load='' size='~~450~~' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>		<StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
	__TOC__		__TOC__
	== Function ==		== Function ==
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	**[[1x5t]] - hPrp subunit 4 – NMR<BR />		**[[1x5t]] - hPrp subunit 4 – NMR<BR />
	**[[5gvq]] - hPrp subunit 4 RNA-binding domain – NMR<BR />		**[[5gvq]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
			**[[6en4]] - hPrp subunits 1,3,5 + SF3B14B<br />
			**[[5ife]] - hPrp subunits 1,3,5 + PHD finger-like protein 5A <br />
			**[[5zya]] - hPrp subunits 1,3,5 + PHD finger-like protein 5A + splicing modulator<br />

	*Pre-mRNA-splicing factor 8		*Pre-mRNA-splicing factor 8
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	**[[3jb9]] – yPrp <br />		**[[3jb9]] – yPrp <br />
	**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]] – yPrp - CryoEM<br />		**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]], [[6g90]], [[6exn]], [[6bk8]], [[5ylz]], [[5y88]] – yPrp - CryoEM<br />
	**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />		**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />

Joel L. Sussman at 18:54, 28 December 2017

2017-12-28T18:54:03Z

← Older revision		Revision as of 18:54, 28 December 2017
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	**[[2do5]] – hPrp subunit 2 – NMR<BR />		**[[2do5]] – hPrp subunit 2 – NMR<BR />
	**[[1x5t]] - hPrp subunit 4 – NMR<BR />		**[[1x5t]] - hPrp subunit 4 – NMR<BR />
	**[[~~1x5u~~]] - hPrp subunit 4 RNA-binding domain – NMR<BR />		**[[5gvq]] - hPrp subunit 4 RNA-binding domain – NMR<BR />

	*Pre-mRNA-splicing factor 8		*Pre-mRNA-splicing factor 8

Joel L. Sussman at 06:29, 16 September 2017

2017-09-16T06:29:40Z

← Older revision		Revision as of 06:29, 16 September 2017
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	<StructureSection load='~~3zef~~' size='450' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>		<StructureSection load='' size='450' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
	__TOC__		__TOC__
	== Function ==		== Function ==

Michal Harel at 09:34, 14 September 2017

2017-09-14T09:34:39Z

← Older revision		Revision as of 09:34, 14 September 2017
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	**[[1x4q]] – hPrp PWI domain - NMR<BR />		**[[1x4q]] – hPrp PWI domain - NMR<BR />
			**[[4yhu]], [[4yhv]] – yPrp C-terminal - yeast<br />
			**[[4yhw]] – yPrp C-terminal + snRNA<br />

	*Pre-mRNA-splicing factor 3B		*Pre-mRNA-splicing factor 3B
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	*Pre-mRNA-splicing factor 8		*Pre-mRNA-splicing factor 8

	**[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 ~~– yeast~~<br />		**[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 <br />
	**[[4jk7]], [[4jk9]], [[3e9o]], [[3e9p]], [[3e66]] - yPrp RNA-binding domain <br />		**[[4jk7]], [[4jk9]], [[3e9o]], [[3e9p]], [[3e66]] - yPrp RNA-binding domain <br />
	**[[4ilj]], [[4jk8]], [[4jka]], [[4jkb]], [[4jkc]], [[4jkd]], [[4jke]], [[4jkf]], [[4jkg]], [[4jkh]] - yPrp RNA-binding domain (mutant)<br />		**[[4ilj]], [[4jk8]], [[4jka]], [[4jkb]], [[4jkc]], [[4jkd]], [[4jke]], [[4jkf]], [[4jkg]], [[4jkh]] - yPrp RNA-binding domain (mutant)<br />
	**[[3zef]], [[4i43]] – yPrp + A1 cistron-splicing factor AAR2<br />		**[[3zef]], [[4i43]] – yPrp + A1 cistron-splicing factor AAR2<br />
	**[[2og4]], [[3sbg]] - yPrp Jab1/MPN domain<br />		**[[2og4]], [[3sbg]] - yPrp Jab1/MPN domain<br />
	**[[4bgd]] – yPrp Jab1/MPN domain + pre-mRNA-splicing helicase BRR2<br />		**[[4bgd]], [[5dca]], [[5m52]], [[5m5p]] – yPrp Jab1/MPN domain + pre-mRNA-splicing helicase BRR2<br />
	**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />		**[[4kit]] – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
	**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />		**[[4ilg]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 <br />
	**[[4bgd]] – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2 + pre-mRNA-splicing helicase BRR2<br />
	**[[3lru]] – hPrp residues 1831-1990<br />		**[[3lru]] – hPrp residues 1831-1990<br />
	**[[3e9l]], [[3enb]] – hPrp endonuclease domain<br />		**[[3e9l]], [[3enb]] – hPrp endonuclease domain<br />
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	**[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />		**[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
			**[[1dvk]] – yPrp functional domain <br />

	*Pre-mRNA-splicing factor 19		*Pre-mRNA-splicing factor 19
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	**[[2ozb]] – hPrp + RNA + tri-snRNP <br />		**[[2ozb]] – hPrp + RNA + tri-snRNP <br />
	**[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />		**[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />

			*Pre-mRNA-splicing factor 38A

			**[[4rz9]], [[4rza]] – hPrp <br />
			**[[5f5s]] – hPrp + microfibrillar-associated protein 1<br />

	*Pre-mRNA-splicing factor 40		*Pre-mRNA-splicing factor 40
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	**[[2xau]], [[3kx2]] – yPrp + ADP		**[[2xau]], [[3kx2]] – yPrp + ADP

			*Pre-mRNA-splicing factors in spliceosome

			**[[3jb9]] – yPrp <br />
			**[[3jcm]], [[5gam]], [[5gan]], [[5gao]], [[5gap]], [[5gmk]], [[5lj3]], [[5lj5]], [[5lqw]], [[5mps]], [[5mq0]], [[5mqf]], [[5wsg]] – yPrp - CryoEM<br />
			**[[3jcr]], [[5o9z]] – hPrp - CryoEM<br />

	*Pre-mRNA-splicing factor cwc2		*Pre-mRNA-splicing factor cwc2

Alexander Berchansky at 08:33, 7 August 2016

2016-08-07T08:33:23Z

← Older revision		Revision as of 08:33, 7 August 2016
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	== Structural highlights ==		== Structural highlights ==
	Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain.		<scene name='46/462200/Cv/2'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.
	</StructureSection>		</StructureSection>