Reverse transcriptase - Revision history

Michal Harel at 11:05, 21 January 2024

2024-01-21T11:05:23Z

← Older revision		Revision as of 11:05, 21 January 2024
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	*[[Efavirenz/emtricitabine/tenofovir]]<br />		*[[Efavirenz/emtricitabine/tenofovir]]<br />
	*[[Phl p 2]]<br />		*[[Phl p 2]]<br />
			*[[Tenofovir disoproxil]]<br />
	*[[AZT-resistant HIV-1 reverse transcriptase]]<br />		*[[AZT-resistant HIV-1 reverse transcriptase]]<br />
	*[[Catalytic Subunit of T. Castaneum TERT Polymerase]].<br />		*[[Catalytic Subunit of T. Castaneum TERT Polymerase]].<br />

Michal Harel at 11:34, 16 January 2024

2024-01-16T11:34:48Z

← Older revision		Revision as of 11:34, 16 January 2024
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	*[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br />		*[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br />
	*[[Efavirenz]]<br />		*[[Efavirenz]]<br />
			*[[Emtricitabine]]<br />
	*[[Efavirenz/emtricitabine/tenofovir]]<br />		*[[Efavirenz/emtricitabine/tenofovir]]<br />
	*[[Phl p 2]]<br />		*[[Phl p 2]]<br />

Michal Harel at 11:33, 16 January 2024

2024-01-16T11:33:32Z

← Older revision		Revision as of 11:33, 16 January 2024
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	*[[Transcription and RNA Processing]]<br />		*[[Transcription and RNA Processing]]<br />
	*[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br />		*[[HIV-1 Reverse Transcriptase in Complex with Nevirapine]]<br />
			*[[Efavirenz]]<br />
			*[[Efavirenz/emtricitabine/tenofovir]]<br />
	*[[Phl p 2]]<br />		*[[Phl p 2]]<br />
	*[[AZT-resistant HIV-1 reverse transcriptase]]<br />		*[[AZT-resistant HIV-1 reverse transcriptase]]<br />

Michal Harel at 07:37, 16 October 2023

2023-10-16T07:37:44Z

← Older revision		Revision as of 07:37, 16 October 2023
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	==Introduction==		==Introduction==
	[[Reverse transcriptase]] (RT) or '''RNA-dependent DNA polymerase''' transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51 subchains. P15 is its RNAse H domain. There are two types of inhibitors for RT: NNRTIs are the non-nucleoside inhibitors, and NRTIs are the ~~nucleoide~~ inhibitors. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, along with [[Hiv protease\|Protease]] and Integrase, the most important part of the protein system involved in the process of infection and reproduction for viruses like HIV, MuLV and AMV. RT has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.		[[Reverse transcriptase]] (RT) or '''RNA-dependent DNA polymerase''' transcribes single-stranded RNA into double-stranded [[DNA]]. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51 subchains. P15 is its RNAse H domain. There are two types of inhibitors for RT: '''NNRTIs''' are the non-nucleoside inhibitors, and '''NRTIs''' are the nucleoside inhibitors. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, along with [[Hiv protease\|Protease]] and Integrase, the most important part of the protein system involved in the process of infection and reproduction for viruses like HIV, MuLV and AMV. RT has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.
	Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries. See also <br />		Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries. See also <br />
	*[[Transcription and RNA Processing]]<br />		*[[Transcription and RNA Processing]]<br />

Alexander Berchansky at 11:18, 24 December 2019

2019-12-24T11:18:36Z

← Older revision		Revision as of 11:18, 24 December 2019
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	*[[Reverse Transcriptase (Hebrew)]]<br />		*[[Reverse Transcriptase (Hebrew)]]<br />

	Reverse Transcriptase is one of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst (see [[UMass Chem 423 Student Projects 2011-2]]) and on display at the [http://www.molecularplayground.org/ Molecular Playground]. <scene name='Reverse_transcriptase/Presentation/3' caption='The hand-like two-enzymes-in-one protein that amazingly makes DNA from RNA'>-- CBI Molecular Playground Model --</scene>		Reverse Transcriptase is one of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst (see [[UMass Chem 423 Student Projects 2011-2#HIV Reverse Transcriptase\|HIV Reverse Transcriptase (UMass Chem 423 Student Projects 2011-2)]]) and on display at the [http://www.molecularplayground.org/ Molecular Playground]. <scene name='Reverse_transcriptase/Presentation/3' caption='The hand-like two-enzymes-in-one protein that amazingly makes DNA from RNA'>-- CBI Molecular Playground Model --</scene>

	{{Clear}}		{{Clear}}

Michal Harel at 10:11, 15 December 2019

2019-12-15T10:11:17Z

← Older revision		Revision as of 10:11, 15 December 2019
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	</StructureSection>		</StructureSection>
	~~== 3D Structures of Reverse transcriptase ==~~

	~~Updated on {{REVISIONDAY2}}-{{MONTHNAME\|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~
	~~{{#tree:id=OrganizedByTopic\|openlevels=0\|~~

	* HIV-1 reverse transcriptase P66/P51

	**[[3dlk]], [[1hmv]] - P66/P51<br />
	**[[1mu2]], [[1jle]], [[1hpz]], [[1hqu]], [[1qe1]], [[1dlo]], [[2wom]], [[2won]], [[3tam]], [[4dg1]], [[4zhr]], [[4ifv]] - P66/P51 (mutant)<br />
	**[[3qip]] - P66/P51 + NNRTI + RNase H inhibitor<br />
	**[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]], [[3t19]], [[5ki4]], [[5fdl]], [[5c24]], [[5c25]], [[4rw7]], [[5ter]], [[5k14]], [[2ynh]], [[2yni]], [[4ncg]], [[1c1b]], [[3lp0]], [[3lp1]], [[3lp2]], [[1vrt]], [[3hvt]], [[4i7f]], [[3m8p]], [[3mec]], [[3mee]], [[2zd1]], [[3qlh]], [[1ikw]], [[1fk9]], [[3dya]], [[3e01]], [[3drp]] - P66/P51+NNRTI<br />
	**[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]], [[3qo9]], [[3t1a]], [[5c42]], [[5tw3]], [[4rw4]], [[4rw6]], [[4rw8]], [[2ynf]], [[2yng]], [[2ynf]], [[2yng]], [[4kko]], [[4lsl]], [[4lsn]], [[4mfb]], [[4o44]], [[4o4g]], [[1lw2]], [[1rt3]], [[6duh]], [[6dug]], [[6duf]], [[6cgf]], [[6c0r]], [[1jla]], [[6c0p]], [[6c0o]], [[6c0n]], [[6c0l]], [[6c0k]], [[6c0j]], [[5vqz]], [[5vqy]], [[5vqx]], [[5vqw]], [[5vqv]], [[5vqu]], [[5vqt]], [[5vqs]], [[5vqr]], [[5vqq]], [[5tep]], [[2ykm]], [[2ykn]], [[4h4m]], [[4h4o]], [[4rw4]], [[4rw6]], [[4rw7]], [[4rw8]], [[4rw9]], [[2iaj]], [[2hnd]], [[2hny]], [[1s1u]], [[1s1x]], [[1lw0]], [[1lwc]], [[1lwe]], [[1lwf]], [[1jlb]], [[1jlf]], [[1fkp]], [[3med]], [[3meg]], [[2ze2]], [[4g1q]], [[4icl]], [[4id5]], [[4idk]], [[4ify]], [[4ig0]], [[4ig3]], [[4kfb]], [[1jkh]], [[1ikv]], [[1fko]], [[3drr]], [[3drs]], [[6dtw]], [[6dtx]] - P66/P51 (mutant)+NNRTI<br />
	**[[4qag]] – Hiv1P66 + coumarin derivative <br />
	**[[1rti]] - P66/P51+ inhibitor<br />
	**[[3ig1]] - P66/P51 + inhibitor beta-thujaplicinol<br />
	**[[3i0r]], [[3i0s]], [[3m8q]], [[3isn]], [[3ith]], [[1c1c]], [[3nbp]], [[5vz6]] - P66/P51+ pyrimidine derivative<br />
	**[[1ikx]], [[4ko0]], [[4we1]], [[6aoc]], [[5tuq]], [[6oe3]] - P66/P51 (mutant)+ pyrimidine derivative<br />
	**[[1tv6]] - P66/P51+ pyridine derivative<br />
	**[[6eli]] - P66/P51+ pyridine derivative + rilpivirine + gag-pol polyprotein<br />
	**[[1jlc]], [[1iky]], [[1eet]] - P66/P51 (mutant) + pyridine derivative<br />
	**[[5cym]], [[5cyq]] - P66/P51 (mutant) + pyrazole derivative<br />
	**[[2b5j]], [[2ban]], [[2be2]], [[1rth]], [[4kv8]] - P66/P51+ pyridone derivative<br />
	**[[5j1e]], [[5hbm]] - P66/P51 (mutant) + pyridone derivative<br />
	**[[6ecl]] - P66/P51 (mutant) + triazole derivative<br />
	**[[6o9e]] - P66/P51 (mutant) + pyridone derivative + CMP<br />
	**[[1tkt]], [[1tkx]], [[1tkz]], [[1tl1]], [[1tl3]] - P66/P51+ quinoline derivative<br />
	**[[5uv5]] - P66/P51 (mutant) + quinoline derivative <br />
	**[[2b6a]] - P66/P51+ THR-50<br />
	**[[2i5j]] - P66/P51 (mutant)+hydrazone derivative<br />
	**[[1rt4]], [[1rt5]], [[1rt6]], [[1rt7]] - P66/P51+ carboxanilide derivative<br />
	**[[1klm]] - P66/P51+piperazine derivative<br />

	* HIV-1 reverse transcriptase complex with DNA/RNA

	**[[3kjv]], [[3kk3]], [[1r0a]], [[1n5y]], [[1n6q]], [[1hys]], [[5d3g]], [[5i3u]] - P66/P51+DNA<br />
	**[[1j5o]], [[1rtd]], [[3klf]], [[3v4i]], [[3v6d]], [[6avt]], [[6avm]], [[6asw]], [[5j2q]], [[5j2p]], [[5j2m]], [[6or7]], [[6otz]], [[6oun]], [[6bhj]] - P66/P51 (mutant)+DNA<br />
	**[[4r5p]], [[5j2n]], [[5txo]], [[5txp]] - P66/P51 (mutant) + nucleotide + DNA<br />
	**[[3v81]] - P66/P51 (mutant) + DNA + NNRTI<br />
	**[[1hvu]] - P66/P51+RNA<br />
	**[[4b3o]], [[4b3p]], [[4b3q]], [[6bsj]], [[6bsi]], [[6bsh]], [[6bsg]] - P66/P51 + RNA + DNA<br />
	**[[4pqu]] - P66/P51 (mutant) + dATP + RNA + DNA<br />
	**[[5i42]] - P66/P51 + dTTP + DNA<br />
	**[[6anq]], [[6an8]], [[6an2]], [[6amo]] - P66/P51 (mutant) + dTTP + DNA<br />
	**[[6p1i]], [[6p1x]], [[6p2g]] - P66/P51 (mutant) + dCTP + DNA<br />
	**[[6ik9]] - P66/P51 (mutant) + dCMP + GTP + DNA<br />
	**[[5txn]], [[5txm]], [[5txl]] - P66/P51 (mutant) + dATP + DNA<br />
	**[[6b19]] - P66/P51 (mutant) + tRNA + RNA<br />
	**[[4puo]], [[4pwd]], [[4q0b]] - P66/P51 (mutant) + NNRTI + RNA + DNA<br />
	**[[1t03]], [[1t05]] - P66/P51+DNA+Fab+NNRTI<br />
	**[[3jsm]] - P66/P51 (mutant)+DNA+NNRTI<br />
	**[[2hmi]] - P66/P51 (mutant)+DNA+Fab<br />
	**[[3kk1]] - P66/P51 (mutant)+DNA + inhibitor<br />
	**[[3kk2]] - P66/P51+DNA+NNRTI<br />
	**[[3jyt]] - P66/P51 (mutant)+DNA+NNRTI<br />
	**[[4r5p]], [[5hlf]], [[5hp1]], [[5hro]] - P66/P51 + DNA + phosphonate inhibitor<br />

	*HIV-1 AZT-resistant reverse transcriptase P66/P51

	**[[3kli]] - P66/P51 (mutant)<br />
	**[[3kle]], [[3klg]], [[3klh]] – P66/P51 (mutant) + DNA

	*HIV-1 reverse transcriptase P66

	**[[2jle]] – P66+NNRTI<br />
	**[[1har]] - P66 N-terminal<br />
	**[[5t82]] - P66 thumb domain - NMR<br />

	*Hiv-1 P15

	**[[1rdh]], [[1hrh]], [[5dzm]] – P15<br />
	**[[3lp3]] – P15+inhibitor MK3<br />
	**[[3k2p]] – P15+inhibitor beta-thujaplicinol<br />
	**[[3hyf]] – P15+pyrimidine derivative<br />
	**[[1o1w]] – P15+Mg - NMR<br />

	*HIV-2 reverse transcriptase

	**[[1mu2]] - RT (mutant)

	* Moloney murine leukemia virus reverse transcriptase

	**[[1mml]] - MmlvRT residues 130-394<br />
	**[[1nnd]] - MmlvRT catalytic fragment residues 144-398 (mutant)<br />
	**[[2r2r]], [[2r2t]], [[2fjv]], [[2fjw]], [[2fjx]], [[1ztw]], [[2fvs]], [[2fvr]], [[2fvq]], [[2fvp]], [[1n4l]] - MmlvRT catalytic fragment residues 144-398 +DNA<br />
	**[[2r2s]], [[2r2u]] – MmlvRT catalytic fragment residues 144-398 +DNA+Co Bleomycin<br />
	**[[1ztt]] – MmlvRT catalytic fragment residues 144-398 +DNA+netropsin<br />
	**[[3fsi]] – MmlvRT catalytic fragment residues 144-398 +DNA+trypanocidal<br />
	**[[2hb5]] – MmlvRT RNAse domain residues 618-791<br />
	**[[4xo0]], [[4xpc]], [[4xpe]], [[5vbs]], [[4m94]], [[4m95]], [[6b1q]], [[6b1r]], [[6b1s]] – MmlvRT catalytic fragment residues 683-937 +DNA<br />
	**[[4mh8]] – MmlvRT P80 residues 683-1330 (mutant)<br />
	**[[1i6j]], [[1d0e]], [[1d1u]], [[1qaj]], [[1qai]], [[6mih]], [[6mik]] – MmlvRT N-terminal+DNA<br />

	*Xenotropic mulv-related virus reverse transcriptase

	**[[3v1o]], [[3v1q]] – RNAse H domain<br />
	**[[3v1r]] – RNAse H domain + inhibitor<br />
	**[[4hkq]] – RT residues 658-1328 (mutant) + DNA + RNA<br />

	*Telomerase reverse transcriptase see [[Telomerase]]

	*other RT

	**[[2lsn]] – RT – Simian foamy virus – NMR<br />
	**[[4ol8]] - P55 (mutant) + RNA + DNA - yeast<br />

	}}
	==See Also==		==See Also==

Michal Harel at 10:10, 15 December 2019

2019-12-15T10:10:08Z

← Older revision		Revision as of 10:10, 15 December 2019
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	One of the principal issues about this protein compared to usual DNA polymerase (besides to the similarity with the Klenow fragment), is the lack of a correction mechanism (usually made by DNA PolIII in the [[User:Karl E. Zahn/RB69 DNA polymerase (GP43)\|DNA Polymerase]]); this deficiency increases the number of errors, producing more mutations and therefore giving more facultative and resistance ability to the virus.		One of the principal issues about this protein compared to usual DNA polymerase (besides to the similarity with the Klenow fragment), is the lack of a correction mechanism (usually made by DNA PolIII in the [[User:Karl E. Zahn/RB69 DNA polymerase (GP43)\|DNA Polymerase]]); this deficiency increases the number of errors, producing more mutations and therefore giving more facultative and resistance ability to the virus.
	{{Clear}}		{{Clear}}

			== 3D Structures of Reverse transcriptase ==
			[[Reverse transcriptase 3D structures]]

	</StructureSection>		</StructureSection>
	== 3D Structures of Reverse transcriptase ==		== 3D Structures of Reverse transcriptase ==
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	**[[3qip]] - P66/P51 + NNRTI + RNase H inhibitor<br />		**[[3qip]] - P66/P51 + NNRTI + RNase H inhibitor<br />
	**[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]], [[3t19]], [[5ki4]], [[5fdl]], [[5c24]], [[5c25]], [[4rw7]], [[5ter]], [[5k14]], [[2ynh]], [[2yni]], [[4ncg]], [[1c1b]], [[3lp0]], [[3lp1]], [[3lp2]], [[1vrt]], [[3hvt]], [[4i7f]], [[3m8p]], [[3mec]], [[3mee]], [[2zd1]], [[3qlh]], [[1ikw]], [[1fk9]], [[3dya]], [[3e01]], [[3drp]] - P66/P51+NNRTI<br />		**[[3irx]], [[3is9]], [[3lak]], [[3lal]], [[3lam]], [[3lan]], [[3ffi]], [[3di6]], [[3dle]], [[3dlg]], [[2rki]], [[3c6t]], [[3c6u]], [[2rf2]], [[2vg5]], [[2vg6]], [[2vg7]], [[2opp]], [[1jlq]], [[1ep4]], [[1c0t]], [[1c0u]], [[1dtt]], [[1dtq]], [[1bqm]], [[1rt1]], [[1rt2]], [[1rev]], [[1rtj]], [[1vru]], [[1hnv]], [[1hni]], [[3t19]], [[5ki4]], [[5fdl]], [[5c24]], [[5c25]], [[4rw7]], [[5ter]], [[5k14]], [[2ynh]], [[2yni]], [[4ncg]], [[1c1b]], [[3lp0]], [[3lp1]], [[3lp2]], [[1vrt]], [[3hvt]], [[4i7f]], [[3m8p]], [[3mec]], [[3mee]], [[2zd1]], [[3qlh]], [[1ikw]], [[1fk9]], [[3dya]], [[3e01]], [[3drp]] - P66/P51+NNRTI<br />
	**[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]], [[3qo9]], [[3t1a]], [[5c42]], [[5tw3]], [[4rw4]], [[4rw6]], [[4rw8]], [[2ynf]], [[2yng]], [[2ynf]], [[2yng]], [[4kko]], [[4lsl]], [[4lsn]], [[4mfb]], [[4o44]], [[4o4g]], [[1lw2]], [[1rt3]], [[6duh]], [[6dug]], [[6duf]], [[6cgf]], [[6c0r]], [[1jla]], [[6c0p]], [[6c0o]], [[6c0n]], [[6c0l]], [[6c0k]], [[6c0j]], [[5vqz]], [[5vqy]], [[5vqx]], [[5vqw]], [[5vqv]], [[5vqu]], [[5vqt]], [[5vqs]], [[5vqr]], [[5vqq]], [[5tep]], [[2ykm]], [[2ykn]], [[4h4m]], [[4h4o]], [[4rw4]], [[4rw6]], [[4rw7]], [[4rw8]], [[4rw9]], [[2iaj]], [[2hnd]], [[2hny]], [[1s1u]], [[1s1x]], [[1lw0]], [[1lwc]], [[1lwe]], [[1lwf]], [[1jlb]], [[1jlf]], [[1fkp]], [[3med]], [[3meg]], [[2ze2]], [[4g1q]], [[4icl]], [[4id5]], [[4idk]], [[4ify]], [[4ig0]], [[4ig3]], [[4kfb]], [[1jkh]], [[1ikv]], [[1fko]], [[3drr]], [[3drs]] - P66/P51 (mutant)+NNRTI<br />		**[[1s1t]], [[1s1v]], [[1s1w]], [[1s6p]], [[1s6q]], [[1s9e]], [[1s9g]], [[1suq]], [[1sv5]], [[3dm2]], [[3dmj]], [[3dok]], [[3dol]], [[2opq]], [[2opr]], [[2ops]], [[2ic3]], [[2hnz]], [[1jlg]], [[1bqn]], [[1uwb]], [[1tvr]], [[3qo9]], [[3t1a]], [[5c42]], [[5tw3]], [[4rw4]], [[4rw6]], [[4rw8]], [[2ynf]], [[2yng]], [[2ynf]], [[2yng]], [[4kko]], [[4lsl]], [[4lsn]], [[4mfb]], [[4o44]], [[4o4g]], [[1lw2]], [[1rt3]], [[6duh]], [[6dug]], [[6duf]], [[6cgf]], [[6c0r]], [[1jla]], [[6c0p]], [[6c0o]], [[6c0n]], [[6c0l]], [[6c0k]], [[6c0j]], [[5vqz]], [[5vqy]], [[5vqx]], [[5vqw]], [[5vqv]], [[5vqu]], [[5vqt]], [[5vqs]], [[5vqr]], [[5vqq]], [[5tep]], [[2ykm]], [[2ykn]], [[4h4m]], [[4h4o]], [[4rw4]], [[4rw6]], [[4rw7]], [[4rw8]], [[4rw9]], [[2iaj]], [[2hnd]], [[2hny]], [[1s1u]], [[1s1x]], [[1lw0]], [[1lwc]], [[1lwe]], [[1lwf]], [[1jlb]], [[1jlf]], [[1fkp]], [[3med]], [[3meg]], [[2ze2]], [[4g1q]], [[4icl]], [[4id5]], [[4idk]], [[4ify]], [[4ig0]], [[4ig3]], [[4kfb]], [[1jkh]], [[1ikv]], [[1fko]], [[3drr]], [[3drs]], [[6dtw]], [[6dtx]] - P66/P51 (mutant)+NNRTI<br />
	**[[4qag]] – Hiv1P66 + coumarin derivative <br />		**[[4qag]] – Hiv1P66 + coumarin derivative <br />
	**[[1rti]] - P66/P51+ inhibitor<br />		**[[1rti]] - P66/P51+ inhibitor<br />
	**[[3ig1]] - P66/P51 + inhibitor beta-thujaplicinol<br />		**[[3ig1]] - P66/P51 + inhibitor beta-thujaplicinol<br />
	**[[3i0r]], [[3i0s]], [[3m8q]], [[3isn]], [[3ith]], [[1c1c]], [[3nbp]], [[5vz6]] - P66/P51+ pyrimidine derivative<br />		**[[3i0r]], [[3i0s]], [[3m8q]], [[3isn]], [[3ith]], [[1c1c]], [[3nbp]], [[5vz6]] - P66/P51+ pyrimidine derivative<br />
	**[[1ikx]], [[4ko0]], [[4we1]], [[6aoc]], [[5tuq]] - P66/P51 (mutant)+ pyrimidine derivative<br />		**[[1ikx]], [[4ko0]], [[4we1]], [[6aoc]], [[5tuq]], [[6oe3]] - P66/P51 (mutant)+ pyrimidine derivative<br />
	**[[1tv6]] - P66/P51+ pyridine derivative<br />		**[[1tv6]] - P66/P51+ pyridine derivative<br />
	**[[6eli]] - P66/P51+ pyridine derivative + rilpivirine + gag-pol polyprotein<br />		**[[6eli]] - P66/P51+ pyridine derivative + rilpivirine + gag-pol polyprotein<br />
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	**[[2b5j]], [[2ban]], [[2be2]], [[1rth]], [[4kv8]] - P66/P51+ pyridone derivative<br />		**[[2b5j]], [[2ban]], [[2be2]], [[1rth]], [[4kv8]] - P66/P51+ pyridone derivative<br />
	**[[5j1e]], [[5hbm]] - P66/P51 (mutant) + pyridone derivative<br />		**[[5j1e]], [[5hbm]] - P66/P51 (mutant) + pyridone derivative<br />
			**[[6ecl]] - P66/P51 (mutant) + triazole derivative<br />
			**[[6o9e]] - P66/P51 (mutant) + pyridone derivative + CMP<br />
	**[[1tkt]], [[1tkx]], [[1tkz]], [[1tl1]], [[1tl3]] - P66/P51+ quinoline derivative<br />		**[[1tkt]], [[1tkx]], [[1tkz]], [[1tl1]], [[1tl3]] - P66/P51+ quinoline derivative<br />
	**[[5uv5]] - P66/P51 (mutant) + quinoline derivative <br />		**[[5uv5]] - P66/P51 (mutant) + quinoline derivative <br />
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	**[[3kjv]], [[3kk3]], [[1r0a]], [[1n5y]], [[1n6q]], [[1hys]], [[5d3g]], [[5i3u]] - P66/P51+DNA<br />		**[[3kjv]], [[3kk3]], [[1r0a]], [[1n5y]], [[1n6q]], [[1hys]], [[5d3g]], [[5i3u]] - P66/P51+DNA<br />
	**[[1j5o]], [[1rtd]], [[3klf]], [[3v4i]], [[3v6d]], [[6avt]], [[6avm]], [[6asw]], [[5j2q]], [[5j2p]], [[5j2m]] - P66/P51 (mutant)+DNA<br />		**[[1j5o]], [[1rtd]], [[3klf]], [[3v4i]], [[3v6d]], [[6avt]], [[6avm]], [[6asw]], [[5j2q]], [[5j2p]], [[5j2m]], [[6or7]], [[6otz]], [[6oun]], [[6bhj]] - P66/P51 (mutant)+DNA<br />
	**[[4r5p]], [[5j2n]], [[5txo]], [[5txp]] - P66/P51 (mutant) + nucleotide + DNA<br />		**[[4r5p]], [[5j2n]], [[5txo]], [[5txp]] - P66/P51 (mutant) + nucleotide + DNA<br />
	**[[3v81]] - P66/P51 (mutant) + DNA + NNRTI<br />		**[[3v81]] - P66/P51 (mutant) + DNA + NNRTI<br />
Line 74:		Line 80:
	**[[5i42]] - P66/P51 + dTTP + DNA<br />		**[[5i42]] - P66/P51 + dTTP + DNA<br />
	**[[6anq]], [[6an8]], [[6an2]], [[6amo]] - P66/P51 (mutant) + dTTP + DNA<br />		**[[6anq]], [[6an8]], [[6an2]], [[6amo]] - P66/P51 (mutant) + dTTP + DNA<br />
			**[[6p1i]], [[6p1x]], [[6p2g]] - P66/P51 (mutant) + dCTP + DNA<br />
			**[[6ik9]] - P66/P51 (mutant) + dCMP + GTP + DNA<br />
	**[[5txn]], [[5txm]], [[5txl]] - P66/P51 (mutant) + dATP + DNA<br />		**[[5txn]], [[5txm]], [[5txl]] - P66/P51 (mutant) + dATP + DNA<br />
	**[[6b19]] - P66/P51 (mutant) + tRNA + RNA<br />		**[[6b19]] - P66/P51 (mutant) + tRNA + RNA<br />
Line 111:		Line 119:

	**[[1mml]] - MmlvRT residues 130-394<br />		**[[1mml]] - MmlvRT residues 130-394<br />
			**[[1nnd]] - MmlvRT catalytic fragment residues 144-398 (mutant)<br />
			**[[2r2r]], [[2r2t]], [[2fjv]], [[2fjw]], [[2fjx]], [[1ztw]], [[2fvs]], [[2fvr]], [[2fvq]], [[2fvp]], [[1n4l]] - MmlvRT catalytic fragment residues 144-398 +DNA<br />
			**[[2r2s]], [[2r2u]] – MmlvRT catalytic fragment residues 144-398 +DNA+Co Bleomycin<br />
			**[[1ztt]] – MmlvRT catalytic fragment residues 144-398 +DNA+netropsin<br />
			**[[3fsi]] – MmlvRT catalytic fragment residues 144-398 +DNA+trypanocidal<br />
	**[[2hb5]] – MmlvRT RNAse domain residues 618-791<br />		**[[2hb5]] – MmlvRT RNAse domain residues 618-791<br />
	**~~[[4mh8]] – MmlvRT P80 residues 683-1330<br />~~		**[[4xo0]], [[4xpc]], [[4xpe]], [[5vbs]], [[4m94]], [[4m95]], [[6b1q]], [[6b1r]], [[6b1s]] – MmlvRT catalytic fragment residues 683-937 +DNA<br />
	**[[2hb5]] - MmlvRT residues 618-791<br />		**[[4mh8]] – MmlvRT P80 residues 683-1330 (mutant)<br />
	**[[1nnd]] - MmlvRT catalytic domain (mutant)<br />		**[[1i6j]], [[1d0e]], [[1d1u]], [[1qaj]], [[1qai]], [[6mih]], [[6mik]] – MmlvRT N-terminal+DNA<br />
	**[[2r2r]], [[2r2t]], [[2fjv]], [[2fjw]], [[2fjx]], [[1ztw]], [[2fvs]], [[2fvr]], [[2fvq]], [[2fvp]], [[1n4l]], [[4xo0]], [[4xpc]], [[4xpe]], [[5vbs]], [[4m94]], [[4m95]] ~~– MmlvRT catalytic domain + DNA<br />~~
	**[[~~2r2s~~]], [[~~2r2u~~]] ~~- MmlvRT catalytic domain +DNA+Co Bleomycin<br />~~
	**[[~~1ztt~~]] - MmlvRT catalytic ~~domain~~ +DNA~~+netropsin~~<br />
	**[[4mh8]] – MmlvRT residues 683-1330 (mutant)<br />
	**[[1i6j]], [[1d0e]], [[1d1u]], [[1qaj]], [[1qai~~]] – MmlvRT N-terminal+DNA<br />~~
	**[[4xpc]], [[~~4xo0~~]], [[~~4xpe~~]] - MmlvRT ~~catalytic domain + DNA<br />~~
	**[[3fsi]] - ~~MmlvRT catalytic domain~~ +DNA~~+trypanocidal<br/ >~~
	**[[5dmq]], [[5dmr]] - MmlvRT + ERF1<br />

	*Xenotropic mulv-related virus reverse transcriptase		*Xenotropic mulv-related virus reverse transcriptase

Michal Harel at 08:16, 14 February 2019

2019-02-14T08:16:24Z

← Older revision		Revision as of 08:16, 14 February 2019
Line 12:		Line 12:
	*[[Reverse Transcriptase (Hebrew)]]<br />		*[[Reverse Transcriptase (Hebrew)]]<br />

	Reverse Transcriptase is one of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst and on display at the [http://www.molecularplayground.org/ Molecular Playground]. <scene name='Reverse_transcriptase/Presentation/3' caption='The hand-like two-enzymes-in-one protein that amazingly makes DNA from RNA'>-- CBI Molecular Playground Model --</scene>		Reverse Transcriptase is one of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst (see [[UMass Chem 423 Student Projects 2011-2]]) and on display at the [http://www.molecularplayground.org/ Molecular Playground]. <scene name='Reverse_transcriptase/Presentation/3' caption='The hand-like two-enzymes-in-one protein that amazingly makes DNA from RNA'>-- CBI Molecular Playground Model --</scene>

	{{Clear}}		{{Clear}}

Michal Harel at 16:12, 25 September 2018

2018-09-25T16:12:54Z

← Older revision		Revision as of 16:12, 25 September 2018
Line 129:		Line 129:
	**[[3v1r]] – RNAse H domain + inhibitor<br />		**[[3v1r]] – RNAse H domain + inhibitor<br />
	**[[4hkq]] – RT residues 658-1328 (mutant) + DNA + RNA<br />		**[[4hkq]] – RT residues 658-1328 (mutant) + DNA + RNA<br />

			*Telomerase reverse transcriptase see [[Telomerase]]

	*other RT		*other RT

Michal Harel at 21:18, 24 September 2018

2018-09-24T21:18:24Z

← Older revision		Revision as of 21:18, 24 September 2018
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	==Structure==		==Structure==

	This ''hand-like'' <scene name='Reverse_transcriptase/Chains/2'>heterodimer</scene> protein has an usual length of 1000 residues (560 in Chain A and 440 for B), a third of them involved in alpha helices and almost a quarter involved in beta sheets, showing α+β <scene name='Reverse_transcriptase/Secondary/2'>secondary structure</scene> domains. <scene name='Reverse_transcriptase/Chaina/2'>Chain A</scene> has an usual weight of 66KDa whereas <scene name='Reverse_transcriptase/Chainb/2'>Chain B</scene> is around 51KDa. These monomers are derived from the same gene, but p51 lacks the amino acids of one active site and has a different tertiary structure conformation compared to p66. Because of this, p51 is enzymatically inactive<ref>PMID: 1377403</ref>.		This ''hand-like'' <scene name='Reverse_transcriptase/Chains/2'>heterodimer</scene> protein has an usual length of 1000 residues (560 in Chain A (shown in red) and 440 for B (shown in green)), a third of them involved in alpha helices and almost a quarter involved in beta sheets, showing α+β <scene name='Reverse_transcriptase/Secondary/2'>secondary structure</scene> domains. <scene name='Reverse_transcriptase/Chaina/2'>Chain A</scene> has an usual weight of 66KDa whereas <scene name='Reverse_transcriptase/Chainb/2'>Chain B</scene> is around 51KDa. These monomers are derived from the same gene, but p51 lacks the amino acids of one active site and has a different tertiary structure conformation compared to p66. Because of this, p51 is enzymatically inactive<ref>PMID: 1377403</ref>.
	There are five distinct structures within the p66 subchain that are used to describe the functions of RT: the fingers (residues 1–85 and 118–155), the palm (residues 86–117 and 156–236), the thumb (residues 237–318), the connection (319–426), and the RNase H (residues 427-end). The palm contains the main active site (residues 110, 185-186)<ref>PMID: 19022262</ref>.		There are five distinct structures within the p66 subchain that are used to describe the functions of RT: the fingers (residues 1–85 and 118–155), the palm (residues 86–117 and 156–236), the thumb (residues 237–318), the connection (319–426), and the RNase H (residues 427-end). The palm contains the main active site (residues 110, 185-186)<ref>PMID: 19022262</ref>.
	{{Clear}}		{{Clear}}