Terminase - Revision history

Michal Harel at 07:52, 18 August 2024

2024-08-18T07:52:14Z

← Older revision		Revision as of 07:52, 18 August 2024
Line 1:		Line 1:
	<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>		<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>

			__TOC__
	== Function ==		== Function ==

	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.		'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits ('''TRM1, TRM2 and TRM3'''), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:52, 19 April 2022

2022-04-19T08:52:19Z

@@ Line 12: / Line 12: @@
 [[Terminase 3D Structures ]]
 </StructureSection>
 == References ==
 <references/>
 [[Category:Topic Page]]

Michal Harel at 08:50, 19 April 2022

2022-04-19T08:50:11Z

← Older revision		Revision as of 08:50, 19 April 2022
Line 9:		Line 9:

	The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.		The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
			== 3D Structures of terminase ==
			[[Terminase 3D Structures ]]
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==
Line 19:		Line 21:
	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />		**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
	**[[6ey7]] – HvTer nuclease domain + inhibitor<br />		**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
	**[[3hef]] – Ter – Bacteriophage T7 <br />		**[[3hef]], [[7lxs]] – Ter – Bacteriophage T7 <br />
	**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />		**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
	**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />		**[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br />
Line 28:		Line 30:
	**[[3p9a]] – Ter – Bacteriophage P22 <br />		**[[3p9a]] – Ter – Bacteriophage P22 <br />
	**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />		**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
	**[[6ejq]] – ~~Ter~~ – Thermus phage<br />		**[[6ejq]] – TpTer – Thermus phage<br />
	**[[~~6hn7~~]] – Ter ~~+ RPPC~~ – ''E. coli'' virus λ <br />		**[[7lw0]] – Ter – ''E. coli'' virus λ <br />
			**[[6hn7]] – Ter + RPPC <br />
	**[[7joq]] – Ter – Pseudomonas phage NV1<br />		**[[7joq]] – Ter – Pseudomonas phage NV1<br />
	**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />		**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />
			**[[7lwr]] – Ter – Enterobacteria phage P21<br />

	*Terminase large subunit		*Terminase large subunit
Line 47:		Line 51:
	**[[4zni]], [[4znk]] – P74Ter ATPase domain <br />		**[[4zni]], [[4znk]] – P74Ter ATPase domain <br />
	**[[4znj]] – P74Ter ATPase domain (mutant)<br />		**[[4znj]] – P74Ter ATPase domain (mutant)<br />
	**[[~~4znl~~]] – ~~P74Ter ATPase domain + ADP~~ <br />		**[[6z6d]], [[6z6e]] – Ter – ''E. coli'' virus H97 <br />

	*Terminase large subunit complex		*Terminase large subunit complex
Line 57:		Line 61:
	**[[5oeb]] – D6eTer + ADP <br />		**[[5oeb]] – D6eTer + ADP <br />
	**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />		**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />
			**[[4znl]] – P74Ter ATPase domain + ADP <br />
			**[[7ks4]] – TpTer ATPase domain + ADP <br />

	*Tripartite terminase		*Tripartite terminase

Michal Harel at 08:37, 23 November 2020

2020-11-23T08:37:54Z

← Older revision		Revision as of 08:37, 23 November 2020
Line 4:		Line 4:
	== Function ==		== Function ==

	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The''' tripartite terminase complex of herpesvirus which ~~contins~~ 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.		'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:37, 23 November 2020

2020-11-23T08:37:04Z

← Older revision		Revision as of 08:37, 23 November 2020
Line 4:		Line 4:
	== Function ==		== Function ==

	'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a small Ter subunit which is a recognition subunit and a large Ter subunit which is an endonuclease/translocase subunit <ref>PMID:~~22297528~~</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP.		'''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The''' tripartite terminase complex of herpesvirus which contins 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>.

	== Structural highlights ==		== Structural highlights ==

Michal Harel at 08:19, 23 November 2020

2020-11-23T08:19:31Z

← Older revision		Revision as of 08:19, 23 November 2020
Line 15:		Line 15:
	{{#tree:id=OrganizedByTopic\|openlevels=0\|		{{#tree:id=OrganizedByTopic\|openlevels=0\|

	*~~Small terminase~~		*Terminase small subunit

	**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />		**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
Line 30:		Line 30:
	**[[6ejq]] – Ter – Thermus phage<br />		**[[6ejq]] – Ter – Thermus phage<br />
	**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />		**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />
			**[[7joq]] – Ter – Pseudomonas phage NV1<br />
			**[[6w7t]] – Ter – Pseudomonas phage PaP3<br />

	*~~Large terminase~~		*Terminase large subunit

	**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />		**[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br />
Line 47:		Line 49:
	**[[4znl]] – P74Ter ATPase domain + ADP <br />		**[[4znl]] – P74Ter ATPase domain + ADP <br />

	*~~Large terminase~~ complex		*Terminase large subunit complex

	**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />		**[[4iee]], [[4ife]] – Sf6Ter + ATP<br />
Line 54:		Line 56:
	**[[5oea]] – D6eTer + ATP <br />		**[[5oea]] – D6eTer + ATP <br />
	**[[5oeb]] – D6eTer + ADP <br />		**[[5oeb]] – D6eTer + ADP <br />
			**[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br />

			*Tripartite terminase

			**[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br />
			**[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br />
			**[[6m5t]] – HvTer TRM3 – Cryo EM<br />
	}}		}}

Michal Harel at 11:12, 13 February 2020

2020-02-13T11:12:05Z

← Older revision		Revision as of 11:12, 13 February 2020
Line 17:		Line 17:
	*Small terminase		*Small terminase

	**[[3n4p]], [[3n4q]] – ~~Ter~~ nuclease domain – Herpesvirus<br />		**[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br />
			**[[6ey7]] – HvTer nuclease domain + inhibitor<br />
	**[[3hef]] – Ter – Bacteriophage T7 <br />		**[[3hef]] – Ter – Bacteriophage T7 <br />
	**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />		**[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br />
Line 26:		Line 27:
	**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />		**[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br />
	**[[3p9a]] – Ter – Bacteriophage P22 <br />		**[[3p9a]] – Ter – Bacteriophage P22 <br />
			**[[6v1i]] – Ter – Thermus virus – Cryo EM<br />
			**[[6ejq]] – Ter – Thermus phage<br />
			**[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br />

	*Large terminase		*Large terminase

Alexander Berchansky at 12:53, 19 September 2019

2019-09-19T12:53:55Z

← Older revision		Revision as of 12:53, 19 September 2019
Line 8:		Line 8:
	== Structural highlights ==		== Structural highlights ==

	The large subunit of Ter is composed of an <scene name='77/775254/Cv/2'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/4'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.		The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==

Michal Harel at 21:56, 24 September 2018

2018-09-24T21:56:09Z

← Older revision		Revision as of 21:56, 24 September 2018
Line 1:		Line 1:
	<StructureSection load='4ife' size='~~450~~' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>		<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'>

Alexander Berchansky at 14:10, 6 December 2017

2017-12-06T14:10:41Z

← Older revision		Revision as of 14:10, 6 December 2017
Line 8:		Line 8:
	== Structural highlights ==		== Structural highlights ==

	The large subunit of Ter is composed of an N-terminal ATPase domain, a linker region and a C-terminal nuclease domain. ATP binds to the protein in a groove between the ATPase domain and the linker region<ref>PMID:23630261</ref>.		The large subunit of Ter is composed of an <scene name='77/775254/Cv/2'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/4'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>.
	</StructureSection>		</StructureSection>
	== 3D Structures of terminase ==		== 3D Structures of terminase ==