User:Stephen Mills/Peptide tutorial 2 - Revision history

Stephen Mills: /* Oligopeptides */

2014-08-21T04:35:25Z

Oligopeptides

← Older revision		Revision as of 04:35, 21 August 2014
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	Now you are ready to work with oligopeptides, or polymers composed of 3-20 amino acid residues. These polymers are linear; that is, each amino acid is linked to it's neighbor in a head-to-tail fashion rather than forming branched chains. You will be observing a 5mer in this tutorial.		Now you are ready to work with oligopeptides, or polymers composed of 3-20 amino acid residues. These polymers are linear; that is, each amino acid is linked to it's neighbor in a head-to-tail fashion rather than forming branched chains. You will be observing a 5mer in this tutorial.

	<Structure load='GDCRY.pdb' size='500' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_start/1' />		<Structure load='GDCRY.pdb' size='500' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_start/1' name='penta'/>

	For this part, you may need to resize and move the molecule around. To remind you:<br/>		For this part, you may need to resize and move the molecule around. To remind you:<br/>
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	'''Identify where Y and R are located in this oligopeptide.'''		'''Identify where Y and R are located in this oligopeptide.'''

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_yr/2'>Click here to have Y and R colored purple.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_yr/2' target='penta'>Click here to have Y and R colored purple.</scene>

	Notice that the NH of Y is bonded to the carbonyl of R.		Notice that the NH of Y is bonded to the carbonyl of R.
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	'''Identify where C and D are located in this peptide.'''		'''Identify where C and D are located in this peptide.'''
	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_cd/2'>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_cd/2' target='penta'>
	Click here to have C and D colored purple.</scene>		Click here to have C and D colored purple.</scene>

Stephen Mills: /* Let's look at another dipeptide. */

2014-08-21T04:34:16Z

Let's look at another dipeptide.

Stephen Mills: /* Last Part!! */

2011-09-07T04:46:04Z

Last Part!!

← Older revision		Revision as of 04:46, 7 September 2011
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	'''Congratulations! You have just finished the first Biochemistry Tutorial'''		'''Congratulations! You have just finished the first Biochemistry Tutorial'''

			[[User:Stephen_Mills/Biochemistry_Tutorials\|Click here to go back to the main Tutorial page.]]

Stephen Mills: /* Last Part!! */

2011-08-30T07:28:09Z

Last Part!!

← Older revision		Revision as of 07:28, 30 August 2011
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	You will probably need to move the molecule around and rotate it to see the whole structure.		You will probably need to move the molecule around and rotate it to see the whole structure.

	When you write it down, it will spell out a question.		When you write down the sequence in the one letter codes, it will spell out a question.

	'''Submit the sequence of the peptide '''''and''''' the answer to the question'''		'''Submit the sequence of the peptide '''''and''''' the answer to the question'''

Stephen Mills: User:Stephen Mills/Sandbox 2 Peptide tutorial 2 moved to User:Stephen Mills/Peptide tutorial 2: Ready for use, keep it protected

2011-08-13T06:16:54Z

User:Stephen Mills/Sandbox 2 Peptide tutorial 2 moved to User:Stephen Mills/Peptide tutorial 2: Ready for use, keep it protected

← Older revision	Revision as of 06:16, 13 August 2011
(No difference)

Stephen Mills: /* Last Part!! */

2011-07-30T06:53:38Z

Last Part!!

← Older revision		Revision as of 06:53, 30 July 2011
Line 65:		Line 65:

	Now a test peptide.		Now a test peptide.
	<Structure load='~~WHENISPIEDAY2~~.pdb' size='600' frame='true' align='left' caption='' scene='~~Insert optional scene name here~~' />		<Structure load='WHENISPIEDAY3.pdb' size='600' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Test_peptide/1' />

	'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>		'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>

Stephen Mills: /* Last Part!! */

2011-07-30T06:46:57Z

Last Part!!

← Older revision		Revision as of 06:46, 30 July 2011
Line 65:		Line 65:

	Now a test peptide.		Now a test peptide.
	<Structure load='~~WHENISPIEDAY~~.pdb' size='600' frame='true' align='left' caption='' scene='Insert optional scene name here' />		<Structure load='WHENISPIEDAY2.pdb' size='600' frame='true' align='left' caption='' scene='Insert optional scene name here' />

	'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>		'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>

Stephen Mills: /* Last Part!! */

2011-07-30T06:41:02Z

Last Part!!

← Older revision		Revision as of 06:41, 30 July 2011
Line 65:		Line 65:

	Now a test peptide.		Now a test peptide.
	<Structure load='WHENISPIEDAY.~~PDB~~' size='600' frame='true' align='left' caption='' scene='~~User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Final_peptide/1~~' />		<Structure load='WHENISPIEDAY.pdb' size='600' frame='true' align='left' caption='' scene='Insert optional scene name here' />

	'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>		'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>

Stephen Mills: /* Last Part!! */

2011-07-30T06:34:05Z

Last Part!!

← Older revision		Revision as of 06:34, 30 July 2011
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	==Last Part!!==		==Last Part!!==

	Now a test peptide.		Now a test peptide.
			<Structure load='WHENISPIEDAY.PDB' size='600' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Final_peptide/1' />

	'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>		'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>

Stephen Mills: /* Oligopeptides */

2011-07-30T06:30:02Z

Oligopeptides

← Older revision		Revision as of 06:30, 30 July 2011
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	<Structure load='GDCRY.pdb' size='500' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_start/1' />		<Structure load='GDCRY.pdb' size='500' frame='true' align='left' caption='' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_start/1' />

	For this part, you may need to resize and move the molecule around. To remind you:		For this part, you may need to resize and move the molecule around. To remind you:<br/>
	To Rotate: left drag		To Rotate: left drag<br/>
	To Zoom: scroll button or shift + left drag		To Zoom: scroll button or shift + left drag<br/>
	To Translate: ctrl + right drag (On a Mac, this doesn't work in FireFox, but does work in Safari).		To Translate: ctrl + right drag (On a Mac, this doesn't work in FireFox, but does work in Safari).<br/>

	'''Identify where Y and R are located in this oligopeptide.'''		'''Identify where Y and R are located in this oligopeptide.'''

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_yr/1'>Click here to have Y and R colored purple.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_yr/2'>Click here to have Y and R colored purple.</scene>

	Notice that the NH of Y is bonded to the carbonyl of R.		Notice that the NH of Y is bonded to the carbonyl of R.
Line 51:		Line 51:

	'''Identify where C and D are located in this peptide.'''		'''Identify where C and D are located in this peptide.'''
	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_cd/1'>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Pentapeptide_cd/2'>
	Click here to have C and D colored purple.</scene>		Click here to have C and D colored purple.</scene>

	Notice how Cysteine's thiol (SH) group points outward from the 5mer backbone at an angle that would allow for the stable formation of the disulfide bond.		Notice how Cysteine's thiol (SH) group points outward from the 5mer backbone at an angle that would allow for the stable formation of the disulfide bond.<br/>

	'''Identify the remaining amino acid in this peptide.'''		'''Identify the remaining amino acid in this peptide.'''<br/>

	'''Write down the short-hand notation for this pentapeptide, in the correct order from the N-terminus to the C-terminus and submit this to your instructor.'''		'''Write down the short-hand notation for this pentapeptide, in the correct order from the N-terminus to the C-terminus and submit this to your instructor.'''

	<br/><br/><br/><br/><br/><br/><br/><br/>		<br/><br/><br/><br/><br/><br/><br/><br/>

			==Last Part!!==

			Now a test peptide.

			'''Identify the sequence of this peptide and submit the answer to your instructor.'''<br/>
			You will probably need to move the molecule around and rotate it to see the whole structure.

			When you write it down, it will spell out a question.

			'''Submit the sequence of the peptide '''''and''''' the answer to the question'''
			<br/><br/><br/><br/>

			'''Congratulations! You have just finished the first Biochemistry Tutorial'''

← Older revision		Revision as of 04:34, 21 August 2014
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	Identify each amino acid in this dipeptide.		Identify each amino acid in this dipeptide.
	<Structure load='CysAsp3.pdb' size='400' frame='true' align='left' caption='Second Dipeptide' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspstart/1' />		<Structure load='CysAsp3.pdb' size='400' frame='true' align='left' caption='Second Dipeptide' scene='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspstart/1' name='CysAsp'/>

	What is the short-hand notation for this dipeptide?		What is the short-hand notation for this dipeptide?

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysasplabels/2'>Click here to show the labels on each amino acid.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysasplabels/2' target='CysAsp'>Click here to show the labels on each amino acid.</scene>

	Identify which amino acid is at the N-terminus and which is at the C-terminus.		Identify which amino acid is at the N-terminus and which is at the C-terminus.

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysasptermini/1'>Click here to label each terminus.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysasptermini/1' target='CysAsp'>Click here to label each terminus.</scene>

	Identify the α carbons of each amino acid.		Identify the α carbons of each amino acid.

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspalphas/1'>Click here to have them highlighted.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspalphas/1' target='CysAsp'>Click here to have them highlighted.</scene>

	Identify the atoms involved in the peptide bond in this dipeptide.		Identify the atoms involved in the peptide bond in this dipeptide.

	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspamide/1'>Click here to have those atoms change color.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspamide/1' target='CysAsp'>Click here to have those atoms change color.</scene>

	Now, measure the distance between the α carbons.		Now, measure the distance between the α carbons.
	<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspmeasure/1'>Click here to get a good starting configuration.</scene>		<scene name='User:Stephen_Mills/Sandbox_2_Peptide_tutorial_2/Cysaspmeasure/1' target='CysAsp'>Click here to get a good starting configuration.</scene>

	To measure a distance, Double click on the first atom, then double click on the second atom. You may need to rotate the molecule a bit to see the distance well. Try it now.		To measure a distance, Double click on the first atom, then double click on the second atom. You may need to rotate the molecule a bit to see the distance well. Try it now.