Ferguson ZNF Sandbox: Difference between revisions
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=====Zinc Ribbon===== | =====Zinc Ribbon===== | ||
In the <scene name='Ferguson_ZNF_Sandbox/Zinc_ribbon/1'>zinc ribbon group</scene>, the ligands for binding are contributed by two zinc knuckles<ref name="four />. The structure contains two beta hairpins, forming two similar sites. One of these hairpins is called the primary beta hairpin and contains the N-terminal zinc sub-site, such as the transcription factor TFIIB and transcriptional elongation factor SII<ref name="four" />. | In the <scene name='Ferguson_ZNF_Sandbox/Zinc_ribbon/1'>zinc ribbon group</scene>, the ligands for binding are contributed by two zinc knuckles<ref name="four" />. The structure contains two beta hairpins, forming two similar sites. One of these hairpins is called the primary beta hairpin and contains the N-terminal zinc sub-site, such as the transcription factor TFIIB and transcriptional elongation factor SII<ref name="four" />. | ||
===Designer Zinc Fingers to Create DNA Scissors=== | ===Designer Zinc Fingers to Create DNA Scissors=== | ||
A breakthrough in the studies of zinc finger proteins has been the ability to cleave sites in a large genome through endonuclease activities<ref name="five">Davis, David, and David Stokoe. "Zinc Finger Nucleases as Tools to Understand and Treat Human Diseases." BMC Medicine 8.1 (2010): 42.</ref>. FokI endonuclease has two domans; one that binds to DNA and another that cleaves the DNA. By fusing a FokI mononmer with two zinc finger proteins, which bind adjacent sequences, will generate at least an 18 base pair sequence specific DNA nuclease that allows for selective targeting in mammalian genomes <ref name="five" /> | A breakthrough in the studies of zinc finger proteins has been the ability to cleave sites in a large genome through endonuclease activities<ref name="five">Davis, David, and David Stokoe. "Zinc Finger Nucleases as Tools to Understand and Treat Human Diseases." BMC Medicine 8.1 (2010): 42.</ref>. FokI endonuclease has two domans; one that binds to DNA and another that cleaves the DNA. By fusing a FokI mononmer with two zinc finger proteins, which bind adjacent sequences, the complex will generate at least an 18 base pair sequence specific DNA nuclease that allows for selective targeting in mammalian genomes <ref name="five" /> | ||
[[Image:ZFN DNA Scissors.jpg|center|400px]] | [[Image:ZFN DNA Scissors.jpg|center|400px]] | ||
