8cel: Difference between revisions

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{{Theoretical_model}}
{{Theoretical_model}}
{{Seed}}
[[Image:8cel.png|left|200px]]


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==THEORETICAL MODEL OF CBH1 IN COMPLEX WITH A CELLULOSE NANOMER==
The line below this paragraph, containing "STRUCTURE_8cel", creates the "Structure Box" on the page.
<StructureSection load='8cel' size='340' side='right' caption='[[8cel]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8CEL FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8cel FirstGlance], [http://www.ebi.ac.uk/pdbsum/8cel PDBsum]</span></td></tr>
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</table>
{{STRUCTURE_8cel|  PDB=8cel  |  SCENE=  }}
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Detailed information has been obtained, by means of protein X-ray crystallography, on how a cellulose chain is bound in the cellulose-binding tunnel of cellobiohydrolase I (CBHI), the major cellulase in the hydrolysis of native, crystalline cellulose by the fungus Trichoderma reesei. Three high-resolution crystal structures of different catalytically deficient mutants of CBHI in complex with cellotetraose, cellopentaose and cellohexaose have been refined at 1.9, 1.7 and 1.9 A resolution, respectively. The observed binding of cellooligomers in the tunnel allowed unambiguous identification of ten well-defined subsites for glucosyl units that span a length of approximately 50 A. All bound oligomers have the same directionality and orientation, and the positions of the glucosyl units in each binding site agree remarkably well between the different complexes. The binding mode observed here corresponds to that expected during productive binding of a cellulose chain. The structures support the hypothesis that hydrolysis by CBHI proceeds from the reducing towards the non-reducing end of a cellulose chain, and they provide a structural explanation for the observed distribution of initial hydrolysis products.


===THEORETICAL MODEL OF CBH1 IN COMPLEX WITH A CELLULOSE NANOMER===
High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei.,Divne C, Stahlberg J, Teeri TT, Jones TA J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:9466911<ref>PMID:9466911</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 8cel" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 9466911 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_9466911}}
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</StructureSection>
==About this Structure==
[[Category: Theoretical Model]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CEL OCA].
 
==Reference==
<ref group="xtra">PMID:9466911</ref><references group="xtra"/>
[[Category: Divne, C]]
[[Category: Divne, C]]
[[Category: Jones, T A]]
[[Category: Jones, T A]]
[[Category: Stahlberg, J]]
[[Category: Stahlberg, J]]
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