Dinoflagellate luciferase: Difference between revisions
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<StructureSection load='1vpr' size='300' side='right' scene='' caption='Luciferase domain from a Dinoflagellate. Selenomethionines shown as space-filling objects [[1vpr]]'> | |||
== Introduction == | == Introduction == | ||
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== Structure == | == Structure == | ||
Composed of residues 868-1218, domain 3 (D3) also consists of a 20aa C-terminal unresolved domain. Containing 7 α-helices and 16 β-strands, D3 is further organized into subdomains. The main portion of the enzyme appears to be a β-barrel structure composed of 10 anti-parallel strands connected via a Gly rich sequence to a 3 helix bundle. This bundle is stabilized by a hydrophobic core region as well as a multitude of H-bonding patterns<ref name="main" />. The β-barrel structure actually has some homology with the human muscle fatty acid binding protein (m-FABP, pdb= | Composed of residues 868-1218, domain 3 (D3) also consists of a 20aa C-terminal unresolved domain. Containing 7 α-helices and 16 β-strands, D3 is further organized into subdomains. The main portion of the enzyme appears to be a β-barrel structure composed of 10 anti-parallel strands connected via a Gly rich sequence to a 3 helix bundle. This bundle is stabilized by a hydrophobic core region as well as a multitude of H-bonding patterns<ref name="main" />. The β-barrel structure actually has some homology with the human muscle fatty acid binding protein (m-FABP, pdb= [[1hmt]]). Both are part of a "β-clam" subdomain family, responsible for binding of hydrophobic molecules. However, other known β-clam structures do not possess enzymatic activity<ref name="main" />. | ||
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[[Image:Luciferase_reaction.jpg]] | [[Image:Luciferase_reaction.jpg|left|thumb|450px]] | ||
''Image courtesy of L. Wayne Schultz.'' | ''Image courtesy of L. Wayne Schultz.'' | ||
== References == | |||
<references /> | |||
==Content Donors== | |||
All the initial portions of this page were created by [[User:James Jones|James Jones]] and were moved because it deserved its own separate page distinct from the associated PDB entry. | |||
</StructureSection> | |||
==3D structures of luciferase== | |||
[[Luciferase]] | [[Luciferase]] | ||
[[1vpr]] is the structure used on this page. | ==See Also== | ||
*[[1vpr]] is the structure used on this page. | |||
[ | *[[Colored & Bioluminescent Proteins]] | ||
* [[PyMOL]] | |||
== External Resources == | |||
[http:// | *[http://www.pdb.org/pdb/explore/explore.do?structureId=1VPR Protein Data Bank file on 1VPR] | ||
== | *[http://www.ncbi.nlm.nih.gov/protein/ABO61076.1?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum NCBI protein entry on ''P. lunula'' luciferase] | ||
*[http://dx.doi.org/10.2210/rcsb_pdb/mom_2006_6 PDB molecule of the month feature on luciferases] | |||
[[Category:Topic Page]] | |||
