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'''Unreleased structure'''


The entry 6ftb is ON HOLD
==Staphylococcus aureus monofunctional glycosyltransferase in complex with moenomycin==
<StructureSection load='6ftb' size='340' side='right' caption='[[6ftb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ftb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_mw2 Staphylococcus aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FTB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1QW:(2R)-2,3-DIHYDROXYPROPYL+DODECANOATE'>1QW</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=M0E:MOENOMYCIN'>M0E</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hzs|3hzs]], [[2c6w|2c6w]], [[2zc5|2zc5]], [[2olu|2olu]], [[3dwk|3dwk]], [[2olv|2olv]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mgt, MW1814 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1242971 Staphylococcus aureus MW2])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidoglycan_glycosyltransferase Peptidoglycan glycosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.129 2.4.1.129] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ftb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ftb OCA], [http://pdbe.org/6ftb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ftb RCSB], [http://www.ebi.ac.uk/pdbsum/6ftb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ftb ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/MGT_STAAW MGT_STAAW]] Involved in the biosynthesis of cell wall peptidoglycan. Responsible for the elongation of the glycan strands using lipid-linked disaccharide-pentapeptide as the substrate (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial peptidoglycan glycosyltransferases (PGT) catalyse the essential polymerization of lipid II into linear glycan chains required for peptidoglycan biosynthesis. The PGT domain is composed of a large head subdomain and a smaller jaw subdomain and can be potently inhibited by the antibiotic moenomycin A (MoeA). We present an X-ray structure of the MoeA-bound Staphylococcus aureus monofunctional PGT enzyme, revealing electron density for a second MoeA bound to the jaw subdomain as well as the PGT donor site. Isothermal titration calorimetry confirms two drug-binding sites with markedly different affinities and positive cooperativity. Hydrophobic cluster analysis suggests that the membrane-interacting surface of the jaw subdomain has structural and physicochemical properties similar to amphipathic cationic alpha -helical antimicrobial peptides for lipid II recognition and binding. Furthermore, molecular dynamics simulations of the drug-free and -bound forms of the enzyme demonstrate the importance of the jaw subdomain movement for lipid II selection and polymerization process and provide molecular-level insights into the mechanism of peptidoglycan biosynthesis by PGTs.


Authors: Punekar, A.S., Dowson, C.J., Roper, D.I.
The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization.,Punekar AS, Samsudin F, Lloyd AJ, Dowson CG, Scott DJ, Khalid S, Roper DI Cell Surf. 2018 Jun;2:54-66. doi: 10.1016/j.tcsw.2018.06.002. PMID:30046666<ref>PMID:30046666</ref>


Description: Staphylococcus aureus monofunctional glycosyltransferase in complex with moenomycin
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Roper, D.I]]
<div class="pdbe-citations 6ftb" style="background-color:#fffaf0;"></div>
[[Category: Punekar, A.S]]
== References ==
[[Category: Dowson, C.J]]
<references/>
__TOC__
</StructureSection>
[[Category: Peptidoglycan glycosyltransferase]]
[[Category: Staphylococcus aureus mw2]]
[[Category: Dowson, C J]]
[[Category: Punekar, A S]]
[[Category: Roper, D I]]
[[Category: Antibiotic]]
[[Category: Cell shape]]
[[Category: Cell wall biosynthesis]]
[[Category: Glycosyltransferase]]
[[Category: Inhibitor]]
[[Category: Membrane]]
[[Category: Moenomycin]]
[[Category: Monofunctional]]
[[Category: Peptidoglycan]]
[[Category: Peptidoglycan synthesis]]
[[Category: Transferase]]
[[Category: Transglycosylase]]
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