6v98

Crystal structure of Type VI secretion system effector, TseH (VCA0285)

spinqualitylabelsall models 6v98, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6v98 is a 1 chain structure with sequence from "bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA NonStd Res: CAF Gene: C9J66_18215, ERS013186_03768, ERS013193_04941, ERS013197_05943, ERS013198_03598, ERS013200_03989, ERS013202_03729, ERS013206_03782, FPV63_00060 ("Bacillo virgola del Koch" Trevisan 1884) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The arms race among microorganisms is a key driver in the evolution of not only the weapons but also defence mechanisms. Many Gram-negative bacteria use the type six secretion system (T6SS) to deliver toxic effectors directly into neighbouring cells. Defence against effectors requires cognate immunity proteins. However, here we show immunity-independent protection mediated by envelope stress responses in Escherichia coli and Vibrio cholerae against a V. cholerae T6SS effector, TseH. We demonstrate that TseH is a PAAR-dependent species-specific effector highly potent against Aeromonas species but not against its V. cholerae immunity mutant or E. coli. A structural analysis reveals TseH is probably a NlpC/P60-family cysteine endopeptidase. We determine that two envelope stress-response pathways, Rcs and BaeSR, protect E. coli from TseH toxicity by mechanisms including capsule synthesis. The two-component system WigKR (VxrAB) is critical for protecting V. cholerae from its own T6SS despite expressing immunity genes. WigR also regulates T6SS expression, suggesting a dual role in attack and defence. This deepens our understanding of how bacteria survive T6SS attacks and suggests that defence against the T6SS represents a major selective pressure driving the evolution of species-specific effectors and protective mechanisms mediated by envelope stress responses and capsule synthesis. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins.,Hersch SJ, Watanabe N, Stietz MS, Manera K, Kamal F, Burkinshaw B, Lam L, Pun A, Li M, Savchenko A, Dong TG Nat Microbiol. 2020 Feb 24. pii: 10.1038/s41564-020-0672-6. doi:, 10.1038/s41564-020-0672-6. PMID:32094588 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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