2r2i: Difference between revisions

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{{Seed}}
[[Image:2r2i.png|left|200px]]


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==Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound==
The line below this paragraph, containing "STRUCTURE_2r2i", creates the "Structure Box" on the page.
<StructureSection load='2r2i' size='340' side='right'caption='[[2r2i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2r2i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R2I FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUCA1A, GCAP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
{{STRUCTURE_2r2i|  PDB=2r2i  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2i OCA], [https://pdbe.org/2r2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r2i RCSB], [https://www.ebi.ac.uk/pdbsum/2r2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2i ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/GUC1A_CHICK GUC1A_CHICK]] Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r2i_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r2i ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Guanylate cyclase-activating proteins (GCAPs) are Ca(2+)-binding proteins myristoylated at the N terminus that regulate guanylate cyclases in photoreceptor cells and belong to the family of neuronal calcium sensors (NCS). Many NCS proteins display a recoverin-like "calcium-myristoyl switch" whereby the myristoyl group, buried inside the protein in the Ca(2+)-free state, becomes fully exposed upon Ca(2+) binding. Here we present a 2.0 A resolution crystal structure of myristoylated GCAP1 with Ca(2+) bound. The acyl group is buried inside Ca(2+)-bound GCAP1. This is in sharp contrast to Ca(2+)-bound recoverin, where the myristoyl group is solvent exposed. Furthermore, we provide direct evidence that the acyl group in GCAP1 remains buried in the Ca(2+)-free state and does not undergo switching. A pronounced kink in the C-terminal helix and the presence of the myristoyl group allow clustering of sequence elements crucial for GCAP1 activity.


===Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound===
Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1.,Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC Structure. 2007 Nov;15(11):1392-402. PMID:17997965<ref>PMID:17997965</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17997965}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2r2i" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 17997965 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17997965}}
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</StructureSection>
==About this Structure==
[[Category: Chick]]
2R2I is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2I OCA].
[[Category: Large Structures]]
 
[[Category: Stephen, R]]
==Reference==
<ref group="xtra">PMID:17997965</ref><references group="xtra"/>
[[Category: Gallus gallus]]
[[Category: Stephen, R.]]
[[Category: Calcium]]
[[Category: Calcium]]
[[Category: Ef hand]]
[[Category: Ef hand]]
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[[Category: Sensory transduction]]
[[Category: Sensory transduction]]
[[Category: Vision]]
[[Category: Vision]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:09:11 2009''
Retrieved from "https://proteopedia.org/2r2i"