5f67: Difference between revisions

Latest revision as of 06:39, 19 July 2023

An exquisitely specific PDZ/target recognition revealed by the structure of INAD PDZ3 in complex with TRP channel tail

Structural highlights

5f67 is a 4 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.76Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

INAD_DROME Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.^[1] ^[2]

Publication Abstract from PubMed

The vast majority of PDZ domains are known to bind to a few C-terminal tail residues of target proteins with modest binding affinities and specificities. Such promiscuous PDZ/target interactions are not compatible with highly specific physiological functions of PDZ domain proteins and their targets. Here, we report an unexpected PDZ/target binding occurring between the scaffold protein inactivation no afterpotential D (INAD) and transient receptor potential (TRP) channel in Drosophila photoreceptors. The C-terminal 15 residues of TRP are required for the specific interaction with INAD PDZ3. The INAD PDZ3/TRP peptide complex structure reveals that only the extreme C-terminal Leu of TRP binds to the canonical alphaB/betaB groove of INAD PDZ3. The rest of the TRP peptide, by forming a beta hairpin structure, binds to a surface away from the alphaB/betaB groove of PDZ3 and contributes to the majority of the binding energy. Thus, the INAD PDZ3/TRP channel interaction is exquisitely specific and represents a new mode of PDZ/target recognitions.

An Exquisitely Specific PDZ/Target Recognition Revealed by the Structure of INAD PDZ3 in Complex with TRP Channel Tail.,Ye F, Liu W, Shang Y, Zhang M Structure. 2016 Jan 27. pii: S0969-2126(16)00006-X. doi:, 10.1016/j.str.2015.12.013. PMID:26853938^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shieh BH, Niemeyer B. A novel protein encoded by the InaD gene regulates recovery of visual transduction in Drosophila. Neuron. 1995 Jan;14(1):201-10. PMID:7826638
↑ Kumar R, Shieh BH. The second PDZ domain of INAD is a type I domain involved in binding to eye protein kinase C. Mutational analysis and naturally occurring variants. J Biol Chem. 2001 Jul 6;276(27):24971-7. Epub 2001 May 7. PMID:11342563 doi:https://dx.doi.org/10.1074/jbc.M103570200
↑ Ye F, Liu W, Shang Y, Zhang M. An Exquisitely Specific PDZ/Target Recognition Revealed by the Structure of INAD PDZ3 in Complex with TRP Channel Tail. Structure. 2016 Jan 27. pii: S0969-2126(16)00006-X. doi:, 10.1016/j.str.2015.12.013. PMID:26853938 doi:https://dx.doi.org/10.1016/j.str.2015.12.013

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Shieh BH, Niemeyer B. A novel protein encoded by the InaD gene regulates recovery of visual transduction in Drosophila. Neuron. 1995 Jan;14(1):201-10. PMID:7826638

[2] Kumar R, Shieh BH. The second PDZ domain of INAD is a type I domain involved in binding to eye protein kinase C. Mutational analysis and naturally occurring variants. J Biol Chem. 2001 Jul 6;276(27):24971-7. Epub 2001 May 7. PMID:11342563 doi:https://dx.doi.org/10.1074/jbc.M103570200

[3] Ye F, Liu W, Shang Y, Zhang M. An Exquisitely Specific PDZ/Target Recognition Revealed by the Structure of INAD PDZ3 in Complex with TRP Channel Tail. Structure. 2016 Jan 27. pii: S0969-2126(16)00006-X. doi:, 10.1016/j.str.2015.12.013. PMID:26853938 doi:https://dx.doi.org/10.1016/j.str.2015.12.013

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==An exquisitely specific PDZ/target recognition revealed by the structure of INAD PDZ3 in complex with TRP channel tail==
-<StructureSection load='5f67' size='340' side='right' caption='[[5f67]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
+<StructureSection load='5f67' size='340' side='right'caption='[[5f67]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5f67]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F67 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5f67]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F67 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f67 OCA], [http://pdbe.org/5f67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f67 RCSB], [http://www.ebi.ac.uk/pdbsum/5f67 PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f67 OCA], [https://pdbe.org/5f67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f67 RCSB], [https://www.ebi.ac.uk/pdbsum/5f67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f67 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/INAD_DROME INAD_DROME]] Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.<ref>PMID:7826638</ref> <ref>PMID:11342563</ref>
+[https://www.uniprot.org/uniprot/INAD_DROME INAD_DROME] Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.<ref>PMID:7826638</ref> <ref>PMID:11342563</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Liu, W]]
+[[Category: Drosophila melanogaster]]
-[[Category: Shang, Y]]
+[[Category: Large Structures]]
-[[Category: Ye, F]]
+[[Category: Liu W]]
-[[Category: Zhang, M]]
+[[Category: Shang Y]]
-[[Category: Atypical]]
+[[Category: Ye F]]
-[[Category: Inad]]
+[[Category: Zhang M]]
-[[Category: Pdz]]
-[[Category: Protein binding]]
-[[Category: Trp]]

5f67: Difference between revisions

Latest revision as of 06:39, 19 July 2023

An exquisitely specific PDZ/target recognition revealed by the structure of INAD PDZ3 in complex with TRP channel tail

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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