2gas: Difference between revisions

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{{Seed}}
[[Image:2gas.png|left|200px]]


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==Crystal Structure of Isoflavone Reductase==
The line below this paragraph, containing "STRUCTURE_2gas", creates the "Structure Box" on the page.
<StructureSection load='2gas' size='340' side='right'caption='[[2gas]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GAS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gas OCA], [https://pdbe.org/2gas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gas RCSB], [https://www.ebi.ac.uk/pdbsum/2gas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gas ProSAT]</span></td></tr>
{{STRUCTURE_2gas|  PDB=2gas  |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/IFR_MEDSA IFR_MEDSA] Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/2gas_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gas ConSurf].
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== Publication Abstract from PubMed ==
Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.


===Crystal Structure of Isoflavone Reductase===
Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.).,Wang X, He X, Lin J, Shao H, Chang Z, Dixon RA J Mol Biol. 2006 May 19;358(5):1341-52. Epub 2006 Mar 29. PMID:16600295<ref>PMID:16600295</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2gas" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16600295 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16600295}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2GAS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].
 
==Reference==
<ref group="xtra">PMID:16600295</ref><references group="xtra"/>
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
[[Category: Chang, Z.]]
[[Category: Chang Z]]
[[Category: Dixon, R A.]]
[[Category: Dixon RA]]
[[Category: He, X.]]
[[Category: He X]]
[[Category: Lin, J.]]
[[Category: Lin J]]
[[Category: Shao, H.]]
[[Category: Shao H]]
[[Category: Wang, X.]]
[[Category: Wang X]]
[[Category: Nadph-dependent reductase]]
 
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Retrieved from "https://proteopedia.org/2gas"