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[[Image:2pyy.jpg|left|200px]]
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{{STRUCTURE_2pyy|  PDB=2pyy  |  SCENE=  }}
'''Crystal Structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with (L)-glutamate'''


==Crystal Structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with (L)-glutamate==
<StructureSection load='2pyy' size='340' side='right'caption='[[2pyy]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pyy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PYY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pyy OCA], [https://pdbe.org/2pyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pyy RCSB], [https://www.ebi.ac.uk/pdbsum/2pyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pyy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D0VWX9_NOSP7 D0VWX9_NOSP7]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/py/2pyy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pyy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GluR0 from Nostoc punctiforme (NpGluR0) is a bacterial homologue of the ionotropic glutamate receptor (iGluR). We have solved the crystal structure of the ligand-binding core of NpGluR0 in complex with l-glutamate at a resolution of 2.1 A. The structure exhibits a noncanonical ligand interaction and two distinct subunit interfaces. The side-chain guanidium group of Arg80 forms a salt bridge with the gamma-carboxyl group of bound L-glutamate: in GluR0 from Synechocystis (SGluR0) and other iGluRs, the equivalent residues are Asn or Thr, which cannot form a similar interaction. We suggest that the local positively charged environment and the steric constraint created by Arg80 mediate the selectivity of L-glutamate binding by preventing the binding of positively charged and hydrophobic amino acids. In addition, the NpGluR0 ligand-binding core forms a new subunit interface in which the two protomers are arranged differently than the known iGluR and SGluR0 dimer interfaces. The significance of there being two different dimer interfaces was investigated using analytical ultracentrifugation analysis.


==Overview==
Crystal structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with L-glutamate: structural dissection of the ligand interaction and subunit interface.,Lee JH, Kang GB, Lim HH, Jin KS, Kim SH, Ree M, Park CS, Kim SJ, Eom SH J Mol Biol. 2008 Feb 15;376(2):308-16. Epub 2007 Nov 6. PMID:18164033<ref>PMID:18164033</ref>
GluR0 from Nostoc punctiforme (NpGluR0) is a bacterial homologue of the ionotropic glutamate receptor (iGluR). We have solved the crystal structure of the ligand-binding core of NpGluR0 in complex with l-glutamate at a resolution of 2.1 A. The structure exhibits a noncanonical ligand interaction and two distinct subunit interfaces. The side-chain guanidium group of Arg80 forms a salt bridge with the gamma-carboxyl group of bound L-glutamate: in GluR0 from Synechocystis (SGluR0) and other iGluRs, the equivalent residues are Asn or Thr, which cannot form a similar interaction. We suggest that the local positively charged environment and the steric constraint created by Arg80 mediate the selectivity of L-glutamate binding by preventing the binding of positively charged and hydrophobic amino acids. In addition, the NpGluR0 ligand-binding core forms a new subunit interface in which the two protomers are arranged differently than the known iGluR and SGluR0 dimer interfaces. The significance of there being two different dimer interfaces was investigated using analytical ultracentrifugation analysis.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2PYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_punctiforme Nostoc punctiforme]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYY OCA].
</div>
<div class="pdbe-citations 2pyy" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with L-glutamate: structural dissection of the ligand interaction and subunit interface., Lee JH, Kang GB, Lim HH, Jin KS, Kim SH, Ree M, Park CS, Kim SJ, Eom SH, J Mol Biol. 2008 Feb 15;376(2):308-16. Epub 2007 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18164033 18164033]
*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
[[Category: Nostoc punctiforme]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Eom, S H.]]
__TOC__
[[Category: Kang, G B.]]
</StructureSection>
[[Category: Lee, J H.]]
[[Category: Large Structures]]
[[Category: Lim, H H.]]
[[Category: Nostoc punctiforme PCC 73102]]
[[Category: Park, C S.]]
[[Category: Eom SH]]
[[Category: Ree, M.]]
[[Category: Kang GB]]
[[Category: Glur0 ligand binding domain]]
[[Category: Lee JH]]
[[Category: Transport protein]]
[[Category: Lim H-H]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:02:32 2008''
[[Category: Park C-S]]
[[Category: Ree M]]

Latest revision as of 11:13, 30 August 2023

Crystal Structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with (L)-glutamate

2pyy, resolution 2.10Å

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