3i8t: Difference between revisions
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New page: '''Unreleased structure''' The entry 3i8t is ON HOLD Authors: Brynda, J., Krejcirikova, V., Rezacova, P. Description: N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose... |
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==N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose== | |||
<StructureSection load='3i8t' size='340' side='right'caption='[[3i8t]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3i8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I8T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i8t OCA], [https://pdbe.org/3i8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i8t RCSB], [https://www.ebi.ac.uk/pdbsum/3i8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i8t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LEG4_MOUSE LEG4_MOUSE] Galectin that binds lactose and a related range of sugars (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Galectin-4, a member of the tandem-repeat subfamily of galectins, participates in cell-membrane interactions and plays an important role in cell adhesion and modulation of immunity and malignity. The oligosaccharide specificity of the mouse galectin-4 carbohydrate-recognition domains (CRDs) has been reported previously. In this work, the structure and binding properties of the N-terminal domain CRD1 were further investigated and the crystal structure of CRD1 in complex with lactose was determined at 2.1 A resolution. The lactose-binding affinity was characterized by fluorescence measurements and two lactose-binding sites were identified: a high-affinity site with a K(d) value in the micromolar range (K(d1) = 600 +/- 70 microM) and a low-affinity site with K(d2) = 28 +/- 10 mM. | |||
Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition.,Krejcirikova V, Pachl P, Fabry M, Maly P, Rezacova P, Brynda J Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):204-11. Epub 2011, Feb 15. PMID:21358051<ref>PMID:21358051</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3i8t" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Galectin 3D structures|Galectin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | |||
[[Category: Brynda J]] | |||
[[Category: Krejcirikova V]] | |||
[[Category: Rezacova P]] | |||
