3iox: Difference between revisions

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{{Seed}}
[[Image:3iox.png|left|200px]]


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==Crystal Structure of A3VP1 of AgI/II of Streptococcus mutans==
The line below this paragraph, containing "STRUCTURE_3iox", creates the "Structure Box" on the page.
<StructureSection load='3iox' size='340' side='right'caption='[[3iox]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3iox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IOX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PMS:PHENYLMETHANESULFONIC+ACID'>PMS</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene></td></tr>
{{STRUCTURE_3iox|  PDB=3iox  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iox OCA], [https://pdbe.org/3iox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iox RCSB], [https://www.ebi.ac.uk/pdbsum/3iox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iox ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8R5D9_STRMG A8R5D9_STRMG]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/3iox_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iox ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Streptococcus mutans antigen I/II (AgI/II) is a cell surface-localized protein adhesin that interacts with salivary components within the salivary pellicle. AgI/II contributes to virulence and has been studied as an immunological and structural target, but a fundamental understanding of its underlying architecture has been lacking. Here we report a high-resolution (1.8 A) crystal structure of the A(3)VP(1) fragment of S. mutans AgI/II that demonstrates a unique fibrillar form (155 A) through the interaction of two noncontiguous regions in the primary sequence. The A(3) repeat of the alanine-rich domain adopts an extended alpha-helix that intertwines with the P(1) repeat polyproline type II (PPII) helix to form a highly extended stalk-like structure heretofore unseen in prokaryotic or eukaryotic protein structures. Velocity sedimentation studies indicate that full-length AgI/II that contains three A/P repeats extends over 50 nanometers in length. Isothermal titration calorimetry revealed that the high-affinity association between the A(3) and P(1) helices is enthalpically driven. Two distinct binding sites on AgI/II to the host receptor salivary agglutinin (SAG) were identified by surface plasmon resonance (SPR). The current crystal structure reveals that AgI/II family proteins are extended fibrillar structures with the number of alanine- and proline-rich repeats determining their length.


===Crystal Structure of A3VP1 of AgI/II of Streptococcus mutans===
Elongated fibrillar structure of a streptococcal adhesin assembled by the high-affinity association of {alpha}- and PPII-helices.,Larson MR, Rajashankar KR, Patel MH, Robinette RA, Crowley PJ, Michalek S, Brady LJ, Deivanayagam C Proc Natl Acad Sci U S A. 2010 Mar 15. PMID:20231452<ref>PMID:20231452</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_20231452}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3iox" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 20231452 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_20231452}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3IOX is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IOX OCA].
 
==Reference==
<ref group="xtra">PMID:20231452</ref><references group="xtra"/>
[[Category: Streptococcus mutans]]
[[Category: Streptococcus mutans]]
[[Category: Brady, L J.]]
[[Category: Brady LJ]]
[[Category: Crowley, P.]]
[[Category: Crowley P]]
[[Category: Deivanayagam, C C.]]
[[Category: Deivanayagam CC]]
[[Category: Larson, M R.]]
[[Category: Larson MR]]
[[Category: Michalek, S M.]]
[[Category: Michalek SM]]
[[Category: Patel, M.]]
[[Category: Patel M]]
[[Category: Rajashankar, K R.]]
[[Category: Rajashankar KR]]
[[Category: Robinette, R.]]
[[Category: Robinette R]]
[[Category: Alpha helix]]
[[Category: Cell adhesion]]
[[Category: Cell wall]]
[[Category: Peptidoglycan-anchor]]
[[Category: Ppii helix]]
[[Category: Supersandwich fold]]
[[Category: Surface adhesin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:32:34 2010''
Retrieved from "https://proteopedia.org/3iox"