3pmk

Crystal structure of the Vesicular Stomatitis Virus RNA free nucleoprotein/phosphoprotein complex

spinqualitylabelsall models 3pmk, resolution 3.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pmk is a 10 chain structure with sequence from Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.03Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B7UCZ2_9RHAB Publication Abstract from PubMed Replication of non-segmented negative-strand RNA viruses requires the continuous supply of the nucleoprotein (N) in the form of a complex with the phosphoprotein (P). Here, we present the structural characterization of a soluble, heterodimeric complex between a variant of vesicular stomatitis virus N lacking its 21 N-terminal residues (N(Delta21)) and a peptide of 60 amino acids (P(60)) encompassing the molecular recognition element (MoRE) of P that binds RNA-free N (N(0)). The complex crystallized in a decameric circular form, which was solved at 3.0 A resolution, reveals how the MoRE folds upon binding to N and competes with RNA binding and N polymerization. Small-angle X-ray scattering experiment and NMR spectroscopy on the soluble complex confirms the binding of the MoRE and indicates that its flanking regions remain flexible in the complex. The structure of this complex also suggests a mechanism for the initiation of viral RNA synthesis. Structure of the vesicular stomatitis virus N-P complex.,Leyrat C, Yabukarski F, Tarbouriech N, Ribeiro EA Jr, Jensen MR, Blackledge M, Ruigrok RW, Jamin M PLoS Pathog. 2011 Sep;7(9):e1002248. Epub 2011 Sep 22. PMID:21960769 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoprotein 3D structures

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