6mn6: Difference between revisions

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<StructureSection load='6mn6' size='340' side='right'caption='[[6mn6]], [[Resolution|resolution]] 3.36&Aring;' scene=''>
<StructureSection load='6mn6' size='340' side='right'caption='[[6mn6]], [[Resolution|resolution]] 3.36&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6mn6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MN6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MN6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6mn6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MN6 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mn6 OCA], [http://pdbe.org/6mn6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mn6 RCSB], [http://www.ebi.ac.uk/pdbsum/6mn6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mn6 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.36&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mn6 OCA], [https://pdbe.org/6mn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mn6 RCSB], [https://www.ebi.ac.uk/pdbsum/6mn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mn6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CNNM3_HUMAN CNNM3_HUMAN]] Probable metal transporter.  
[https://www.uniprot.org/uniprot/CNNM3_HUMAN CNNM3_HUMAN] Probable metal transporter.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The family of cystathionine-beta-synthase (CBS)-pair domain divalent metal cation transport mediators (CNNMs) is composed of four integral membrane proteins associated with Mg(2+) transport. Structurally, CNNMs contain large cytosolic regions composed of a CBS-pair and a cyclic nucleotide-binding homology (CNBH) domain. How these regulate Mg(2+) transport activity is unknown. Here, we determined the crystal structures of cytosolic fragments in two conformations: Mg(2+)-ATP-analog bound and ligand free. The structures reveal open and closed conformations with functionally important contacts not observed in structures of the individual domains. We also identified a second Mg(2+)-binding region in the CBS-pair domain and a different dimerization interface for the CNBH domain. Analytical ultracentrifugation and isothermal titration calorimetry experiments revealed a tight correlation between Mg(2+)-ATP binding and protein dimerization. Mutations that blocked either function prevented cellular Mg(2+) efflux activity. The results suggest Mg(2+) efflux is regulated by conformational changes associated with Mg(2+)-ATP binding to CNNM CBS-pair domains.
 
Mg(2+)-ATP Sensing in CNNM, a Putative Magnesium Transporter.,Chen YS, Kozlov G, Fakih R, Yang M, Zhang Z, Kovrigin EL, Gehring K Structure. 2019 Dec 10. pii: S0969-2126(19)30434-4. doi:, 10.1016/j.str.2019.11.016. PMID:31864811<ref>PMID:31864811</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6mn6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chen, Y S]]
[[Category: Chen YS]]
[[Category: Gehring, K]]
[[Category: Gehring K]]
[[Category: Yang, M]]
[[Category: Yang M]]
[[Category: Cnnm3]]
[[Category: Cyclic nucleotide-binding homology domain]]
[[Category: Cystathionine-beta-synthase domain]]
[[Category: Membrane protein]]
[[Category: Metal transport]]
[[Category: Mg2+ transporter]]
[[Category: Open conformation]]
Retrieved from "https://proteopedia.org/6mn6"