7mi2: Difference between revisions

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New page: '''Unreleased structure''' The entry 7mi2 is ON HOLD until Paper Publication Authors: Jacewicz, A., Shuman, S. Description: Seb1-G476S RNA binding domain [[Category: Unreleased Structu...
 
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'''Unreleased structure'''


The entry 7mi2 is ON HOLD  until Paper Publication
==Seb1-G476S RNA binding domain==
<StructureSection load='7mi2' size='340' side='right'caption='[[7mi2]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7mi2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MI2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mi2 OCA], [https://pdbe.org/7mi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mi2 RCSB], [https://www.ebi.ac.uk/pdbsum/7mi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mi2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEB1_SCHPO SEB1_SCHPO] Involved in the processing of pol II transcripts.<ref>PMID:12907709</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The system of long noncoding RNA (lncRNA)-mediated transcriptional interference that represses fission yeast phosphate homoeostasis gene pho1 provides a sensitive readout of genetic influences on cotranscriptional 3'-processing and termination and a tool for discovery of regulators of this phase of the Pol2 transcription cycle. Here, we conducted a genetic screen for relief of transcriptional interference that unveiled a mechanism by which Pol2 termination is enhanced via a gain-of-function mutation, G476S, in the RNA-binding domain of an essential termination factor, Seb1. The genetic and physical evidence for gain-of-function is compelling: 1) seb1-G476S de-represses pho1 and tgp1, both of which are subject to lncRNA-mediated transcriptional interference; 2) seb1-G476S elicits precocious lncRNA transcription termination in response to lncRNA 5'-proximal poly(A) signals; 3) seb1-G476S derepression of pho1 is effaced by loss-of-function mutations in cleavage and polyadenylation factor (CPF) subunits and termination factor Rhn1; 4) synthetic lethality of seb1-G476S with pho1 derepressive mutants rpb1-CTD-S7A and aps1 is rescued by CPF/Rhn1 loss-of-function alleles; and 5) seb1-G476S elicits an upstream shift in poly(A) site preference in several messenger RNA genes. A crystal structure of the Seb1-G476S RNA-binding domain indicates potential for gain of contacts from Ser476 to RNA nucleobases. To our knowledge, this is a unique instance of a gain-of-function phenotype in a eukaryal transcription termination protein.


Authors: Jacewicz, A., Shuman, S.
Genetic screen for suppression of transcriptional interference identifies a gain-of-function mutation in Pol2 termination factor Seb1.,Schwer B, Garg A, Jacewicz A, Shuman S Proc Natl Acad Sci U S A. 2021 Aug 17;118(33). pii: 2108105118. doi:, 10.1073/pnas.2108105118. PMID:34389684<ref>PMID:34389684</ref>


Description: Seb1-G476S RNA binding domain
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Shuman, S]]
<div class="pdbe-citations 7mi2" style="background-color:#fffaf0;"></div>
[[Category: Jacewicz, A]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Jacewicz A]]
[[Category: Shuman S]]
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