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==Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), tetragonal form, to 3.6 Ang resolution==
==Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), tetragonal form, to 3.6 Ang resolution==
<StructureSection load='3dky' size='340' side='right' caption='[[3dky]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<StructureSection load='3dky' size='340' side='right'caption='[[3dky]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3dky]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DKY FirstGlance]. <br>
<table><tr><td colspan='2'>[[3dky]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DKY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dkx|3dkx]], [[1l2m|1l2m]], [[2hw0|2hw0]], [[1ksx|1ksx]], [[1ksy|1ksy]], [[2gxa|2gxa]], [[1rz9|1rz9]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">repB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1311 Streptococcus agalactiae])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dky OCA], [https://pdbe.org/3dky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dky RCSB], [https://www.ebi.ac.uk/pdbsum/3dky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dky ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dky OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dky RCSB], [http://www.ebi.ac.uk/pdbsum/3dky PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/REPB_STRAG REPB_STRAG]] Is essential for plasmid replication. Nicks the positive strand at the plus origin of replication.  
[https://www.uniprot.org/uniprot/REPB_STRAG REPB_STRAG] Is essential for plasmid replication. Nicks the positive strand at the plus origin of replication.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dk/3dky_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dk/3dky_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dky ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 3dky" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus agalactiae]]
[[Category: Streptococcus agalactiae]]
[[Category: Blanco, A G]]
[[Category: Blanco AG]]
[[Category: Boer, D R]]
[[Category: Boer DR]]
[[Category: Coll, M]]
[[Category: Coll M]]
[[Category: Espinosa, M]]
[[Category: Del Solar G]]
[[Category: Gomis-Ruth, F X]]
[[Category: Espinosa M]]
[[Category: Ruiz-Maso, J A]]
[[Category: Gomis-Ruth FX]]
[[Category: Solar, G Del]]
[[Category: Ruiz-Maso JA]]
[[Category: Uson, I]]
[[Category: Uson I]]
[[Category: Vives-Llacer, M]]
[[Category: Vives-Llacer M]]
[[Category: Dna replication]]
[[Category: Flexible nuclease domain]]
[[Category: Hexamer]]
[[Category: Nuclease]]
[[Category: Plasmid replication]]
[[Category: Replication]]
[[Category: Replication initiation]]
[[Category: Replication initiator]]

Latest revision as of 15:11, 1 November 2023

Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), tetragonal form, to 3.6 Ang resolution

3dky, resolution 3.60Å

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