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{{STRUCTURE_3la6|  PDB=3la6  |  SCENE=  }}
===Octameric kinase domain of the E. coli tyrosine kinase Wzc with bound ADP===
{{ABSTRACT_PUBMED_20633230}}


==Function==
==Octameric kinase domain of the E. coli tyrosine kinase Wzc with bound ADP==
[[http://www.uniprot.org/uniprot/WZC_ECOLI WZC_ECOLI]] Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium. Phosphorylates udg.  
<StructureSection load='3la6' size='340' side='right'caption='[[3la6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3la6]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LA6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3la6 OCA], [https://pdbe.org/3la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3la6 RCSB], [https://www.ebi.ac.uk/pdbsum/3la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3la6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/WZC_ECOLI WZC_ECOLI] Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium. Phosphorylates udg.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/3la6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3la6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export are regulated within the transmembrane polysaccharide assembly machinery by the autophosphorylation of atypical tyrosine-kinases, named BY-kinases. However, the accurate functioning of these tyrosine-kinases remains unknown. Here, we report the crystal structure of the non-phosphorylated cytoplasmic domain of the tyrosine-kinase Wzc from Escherichia coli in complex with ADP showing that it forms a ring-shaped octamer. Mutational analysis demonstrates that a conserved EX(2)RX(2)R motif involved in subunit interactions is essential for polysaccharide export. We also elucidate the role of a putative internal regulatory tyrosine and we show that BY-kinases from proteobacteria autophosphorylate on their C-terminal tyrosine cluster via a single step intermolecular mechanism. This structure-function analysis also allows us to demonstrate that two different parts of a conserved basic region called the RK-cluster are essential for polysaccharide export and for kinase activity, respectively. Based on these data, we revisit the dichotomy made between BY-kinases from proteobacteria and firmicutes and we propose a unique process of oligomerization and phosphorylation. We also reassess the function of BY-kinases in the capsular polysaccharide assembly machinery.


==About this Structure==
Identification of structural and molecular determinants of the tyrosine-kinase Wzc and implications in capsular polysaccharide export.,Bechet E, Gruszczyk J, Terreux R, Gueguen-Chaignon V, Vigouroux A, Obadia B, Cozzone AJ, Nessler S, Grangeasse C Mol Microbiol. 2010 Jul 13. PMID:20633230<ref>PMID:20633230</ref>
[[3la6]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LA6 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:020633230</ref><references group="xtra"/><references/>
</div>
[[Category: Ecoli]]
<div class="pdbe-citations 3la6" style="background-color:#fffaf0;"></div>
[[Category: Bechet, E.]]
 
[[Category: Grangeasse, C.]]
==See Also==
[[Category: Gruszczyk, J.]]
*[[Tyrosine kinase 3D structures|Tyrosine kinase 3D structures]]
[[Category: Gueguen-Chaignon, V.]]
== References ==
[[Category: Nessler, S.]]
<references/>
[[Category: Vigouroux, A.]]
__TOC__
[[Category: Bacterial protein kinase]]
</StructureSection>
[[Category: Exopolysaccharide synthesis]]
[[Category: Escherichia coli K-12]]
[[Category: Intermolecular phosphorylation]]
[[Category: Large Structures]]
[[Category: Nucleotide binding domain]]
[[Category: Bechet E]]
[[Category: Oligomerization]]
[[Category: Grangeasse C]]
[[Category: P-loop protein]]
[[Category: Gruszczyk J]]
[[Category: Transferase]]
[[Category: Gueguen-Chaignon V]]
[[Category: Walker a motif]]
[[Category: Nessler S]]
[[Category: Vigouroux A]]
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