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[[Image:2btj.gif|left|200px]]
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{{STRUCTURE_2btj|  PDB=2btj  |  SCENE=  }}
'''FLUORESCENT PROTEIN EOSFP- RED FORM'''


==Fluorescent Protein EosFP - red form==
<StructureSection load='2btj' size='340' side='right'caption='[[2btj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2btj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BTJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene>, <scene name='pdbligand=RC7:{(2R)-4-(4-HYDROXYBENZYL)-2-[2-(1H-IMIDAZOL-4-YL)ETHYL]-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETALDEHYDE'>RC7</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2btj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2btj OCA], [https://pdbe.org/2btj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2btj RCSB], [https://www.ebi.ac.uk/pdbsum/2btj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2btj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bt/2btj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2btj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.


==Overview==
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.,Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:15964985<ref>PMID:15964985</ref>
Genetically encoded fusion constructs derived from fluorescent proteins (FPs) can be designed to report on a multitude of events and signals in cells, tissues, and entire organs without interfering with the complex machinery of life. EosFP is a novel FP from the scleractinian coral Lobophyllia hemprichii that switches its fluorescence emission from green (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light. This property enables localized tagging of proteins and thus provides a valuable tool for tracking protein movements within live cells. Here, we present the x-ray structures of the green and red forms of WT EosFP. They reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2BTJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BTJ OCA].
</div>
<div class="pdbe-citations 2btj" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP., Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15964985 15964985]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lobophyllia hemprichii]]
[[Category: Lobophyllia hemprichii]]
[[Category: Single protein]]
[[Category: Nar H]]
[[Category: Nar, H.]]
[[Category: Nienhaus GU]]
[[Category: Nienhaus, G U.]]
[[Category: Nienhaus K]]
[[Category: Nienhaus, K.]]
[[Category: Wiedenmann J]]
[[Category: Wiedenmann, J.]]
[[Category: Fluorescent protein]]
[[Category: Green-to-red conversion]]
[[Category: Luminescent protein]]
[[Category: Photo-induced protein cleavage]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:46:42 2008''
Retrieved from "https://proteopedia.org/2btj"