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==Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump==
==Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump==
<StructureSection load='5nik' size='340' side='right'caption='[[5nik]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<SX load='5nik' size='340' side='right' viewer='molstar' caption='[[5nik]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5nik]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NIK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NIK FirstGlance]. <br>
<table><tr><td colspan='2'>[[5nik]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NIK FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tolC, colE1-i, mtcB, mukA, refI, toc, weeA, b3035, JW5503 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), macA, ybjY, b0878, JW0862 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), macB, ybjZ, b0879, JW0863 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nik OCA], [http://pdbe.org/5nik PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nik RCSB], [http://www.ebi.ac.uk/pdbsum/5nik PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nik ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nik OCA], [https://pdbe.org/5nik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nik RCSB], [https://www.ebi.ac.uk/pdbsum/5nik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nik ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TOLC_ECOLI TOLC_ECOLI]] Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.<ref>PMID:6337123</ref> <ref>PMID:11274125</ref> <ref>PMID:15228545</ref> <ref>PMID:18955484</ref> <ref>PMID:23176499</ref> [[http://www.uniprot.org/uniprot/MACB_ECOLI MACB_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:23974027</ref>  [[http://www.uniprot.org/uniprot/MACA_ECOLI MACA_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacA stimulates the ATPase activity of MacB by promoting the closed ATP-bound state of MacB, increases the capacity of MacB to bind macrolides such as erythromycin, and provides a physical link between MacB and TolC. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, MacA binds tightly rough-core lipopolysaccharide (R-LPS), suggesting that the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:21696464</ref> <ref>PMID:23974027</ref> 
[https://www.uniprot.org/uniprot/TOLC_ECOLI TOLC_ECOLI] Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.<ref>PMID:6337123</ref> <ref>PMID:11274125</ref> <ref>PMID:15228545</ref> <ref>PMID:18955484</ref> <ref>PMID:23176499</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bai, X C]]
[[Category: Bai X-C]]
[[Category: Blaza, J N]]
[[Category: Blaza JN]]
[[Category: Du, D]]
[[Category: Du D]]
[[Category: Fitzpatrick, A W.P]]
[[Category: Fitzpatrick AWP]]
[[Category: Llabres, S]]
[[Category: Llabres S]]
[[Category: Luisi, B F]]
[[Category: Luisi BF]]
[[Category: Murakami, S]]
[[Category: Murakami S]]
[[Category: Neuberger, A]]
[[Category: Neuberger A]]
[[Category: Okada, U]]
[[Category: Okada U]]
[[Category: Scheres, S H.W]]
[[Category: Scheres SHW]]
[[Category: Veen, H W.van]]
[[Category: Zachariae U]]
[[Category: Zachariae, U]]
[[Category: Van Veen HW]]
[[Category: Abc transporter]]
[[Category: Drug efflux pump]]
[[Category: Macrolide transporter]]
[[Category: Multi-drug resistance]]
[[Category: Toxin transporter]]
[[Category: Transport protein]]
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