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New page: left|200px<br /><applet load="1evm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1evm" /> '''NMR OBSERVATION OF A-TETRAD'''<br /> ==Over...
 
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[[Image:1evm.gif|left|200px]]<br /><applet load="1evm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1evm" />
'''NMR OBSERVATION OF A-TETRAD'''<br />


==Overview==
==NMR OBSERVATION OF A-TETRAD==
The structure of the telomeric DNA has been a subject of extensive, investigation in recent years due to the realization that it has important, functional roles to play in vivo and the observations that truncated, telomeric sequences exhibit a great variety of 3D structures in aqueous, solutions. In this context, we describe here NMR structural studies on two, truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in, solutions containing K(+)ions. The G(3)stretches in both the, oligonucleotides were seen to form parallel-stranded quadruplexes., However, the AG(3)segment as a whole, had different structural, characteristics. The structure of d-AG(3)T revealed the formation of a, novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had, syn glycosidic conformation as opposed to the anti conformation of the G's, in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and, the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T., These observations are expected to be significant from the point of view, of structural diversity and recognition in telomeres.
<StructureSection load='1evm' size='340' side='right'caption='[[1evm]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1evm]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EVM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1evm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evm OCA], [https://pdbe.org/1evm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1evm RCSB], [https://www.ebi.ac.uk/pdbsum/1evm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1evm ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.


==About this Structure==
NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad.,Patel PK, Koti AS, Hosur RV Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:10481022<ref>PMID:10481022</ref>
1EVM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EVM OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10481022 10481022]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1evm" style="background-color:#fffaf0;"></div>
[[Category: Hosur, R.V.]]
== References ==
[[Category: Koti, A.S.R.]]
<references/>
[[Category: Patel, P.K.]]
__TOC__
[[Category: a-tetrad]]
</StructureSection>
[[Category: g-quadruplex]]
[[Category: Large Structures]]
[[Category: human telomere]]
[[Category: Hosur RV]]
[[Category: nmr structure]]
[[Category: Koti ASR]]
 
[[Category: Patel PK]]
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