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==Structural and kinetic characterization of Porphyromonas gingivalis glutaminyl cyclase==
==Structural and kinetic characterization of Porphyromonas gingivalis glutaminyl cyclase==
<StructureSection load='7cjg' size='340' side='right'caption='[[7cjg]]' scene=''>
<StructureSection load='7cjg' size='340' side='right'caption='[[7cjg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CJG FirstGlance]. <br>
<table><tr><td colspan='2'>[[7cjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis_W83 Porphyromonas gingivalis W83]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CJG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cjg OCA], [https://pdbe.org/7cjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cjg RCSB], [https://www.ebi.ac.uk/pdbsum/7cjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cjg ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMD:5,6-DIMETHYLBENZIMIDAZOLE'>DMD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cjg OCA], [https://pdbe.org/7cjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cjg RCSB], [https://www.ebi.ac.uk/pdbsum/7cjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cjg ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7MT37_PORGI Q7MT37_PORGI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Porphyromonas gingivalis is a bacterial species known to be involved in the pathogenesis of chronic periodontitis, that more recently has been as well associated with Alzheimer's disease. P. gingivalis expresses a glutaminyl cyclase (PgQC) whose human ortholog is known to participate in the beta amyloid peptide metabolism. We have elucidated the crystal structure of PgQC at 1.95 A resolution in unbound and in inhibitor-complexed forms. The structural characterization of PgQC confirmed that PgQC displays a mammalian fold rather than a bacterial fold. Our biochemical characterization indicates that PgQC uses a mammalian-like catalytic mechanism enabled by the residues Asp(149), Glu(182), Asp(183), Asp(218), Asp(267) and His(299). In addition, we could observe that a non-conserved Trp(193) may drive differences in the binding affinity of ligands which might be useful for drug development. With a screening of a small molecule library, we have identified a benzimidazole derivative rendering PgQC inhibition in the low micromolar range that might be amenable for further medicinal chemistry development.
Structural and kinetic characterization of Porphyromonas gingivalis glutaminyl cyclase.,Lamers S, Feng Q, Cheng Y, Yu S, Sun B, Lukman M, Jiang J, Ruiz-Carrillo D Biol Chem. 2021 Apr 7. pii: hsz-2020-0298. doi: 10.1515/hsz-2020-0298. PMID:33823093<ref>PMID:33823093</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7cjg" style="background-color:#fffaf0;"></div>
==See Also==
*[[Glutaminyl cyclase|Glutaminyl cyclase]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Porphyromonas gingivalis W83]]
[[Category: Feng Q]]
[[Category: Feng Q]]
[[Category: Jiang J]]
[[Category: Jiang J]]
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