7ya2

Crystal structure of capsular polysaccharide synthesis enzyme CapG from Staphylococcus aureus

spinqualitylabelsall models 7ya2, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ya2 is a 6 chain structure with sequence from Staphylococcus aureus subsp. aureus str. Newman. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.2Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3K5M7_STAAE Publication Abstract from PubMed Bacterial capsular polysaccharides provide protection against environmental stress and immune evasion from the host immune system, and are therefore considered to be attractive therapeutic targets for the development of anti-infectious reagents. Here, we focused on CapG, one of the key enzymes in the synthesis pathway of capsular polysaccharides type 5 (CP5) from the opportunistic pathogen Staphylococcus aureus. SaCapG catalyses the 2-epimerization of UDP-N-acetyl-D-talosamine (UDP-TalNAc) to UDP-N-acetyl-D-fucosamine (UDP-FucNAc), which is one of the nucleotide-activated precursors for the synthesis of the trisaccharide repeating units of CP5. Here, the cloning, expression and purification of recombinant SaCapG are reported. After extensive efforts, single crystals of SaCapG were successfully obtained which belonged to space group C2 and exhibited unit-cell parameters a = 302.91, b = 84.34, c = 145.09 A, beta = 110.65 degrees . The structure was solved by molecular replacement and was refined to 3.2 A resolution. The asymmetric unit revealed a homohexameric assembly of SaCapG, which was consistent with gel-filtration analysis. Structural comparison with UDP-N-acetyl-D-glucosamine 2-epimerase from Methanocaldococcus jannaschii identified alpha2, the alpha2-alpha3 loop and alpha10 as a gate-regulated switch controlling substrate entry and/or product release. Crystal structure of the capsular polysaccharide-synthesis enzyme CapG from Staphylococcus aureus.,Tien N, Ho CY, Lai SJ, Lin YC, Yang CS, Wang YC, Huang WC, Chen Y, Chang JJ Acta Crystallogr F Struct Biol Commun. 2022 Nov 1;78(Pt 11):378-385. doi:, 10.1107/S2053230X22008743. Epub 2022 Oct 14. PMID:36322423 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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