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[[Image:1ear.gif|left|200px]]


{{Structure
==Crystal structure of Bacillus pasteurii UreE at 1.7 A. Type II crystal form.==
|PDB= 1ear |SIZE=350|CAPTION= <scene name='initialview01'>1ear</scene>, resolution 1.7&Aring;
<StructureSection load='1ear' size='340' side='right'caption='[[1ear]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A+Symmetry+Related+Subunits+Co+...'>AC1</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[1ear]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EAR FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ear OCA], [https://pdbe.org/1ear PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ear RCSB], [https://www.ebi.ac.uk/pdbsum/1ear PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ear ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ear FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ear OCA], [http://www.ebi.ac.uk/pdbsum/1ear PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ear RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/UREE_SPOPA UREE_SPOPA] Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/1ear_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ear ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly.


'''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.7 A. TYPE II CRYSTAL FORM.'''
Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.,Remaut H, Safarov N, Ciurli S, Van Beeumen J J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602<ref>PMID:11602602</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ear" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly.
*[[Urease accessory protein|Urease accessory protein]]
 
== References ==
==About this Structure==
<references/>
1EAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAR OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11602602 11602602]
[[Category: Single protein]]
[[Category: Sporosarcina pasteurii]]
[[Category: Sporosarcina pasteurii]]
[[Category: Beeumen, J Van.]]
[[Category: Ciurli S]]
[[Category: Ciurli, S.]]
[[Category: Remaut H]]
[[Category: Remaut, H.]]
[[Category: Safarov N]]
[[Category: Safarov, N.]]
[[Category: Van Beeumen J]]
[[Category: putative ni-chaperone]]
[[Category: urease operon]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:58:53 2008''
Retrieved from "https://proteopedia.org/1ear"