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==Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis==
==Crystal Structure of the Prothrombinase Complex from the Venom of Pseudonaja Textilis==
<StructureSection load='4bxs' size='340' side='right' caption='[[4bxs]], [[Resolution|resolution]] 3.32&Aring;' scene=''>
<StructureSection load='4bxs' size='340' side='right'caption='[[4bxs]], [[Resolution|resolution]] 3.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4bxs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eastern_brown_snake Eastern brown snake]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BXS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4bxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonaja_textilis Pseudonaja textilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BXS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.32&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bxw|4bxw]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxs OCA], [http://pdbe.org/4bxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bxs RCSB], [http://www.ebi.ac.uk/pdbsum/4bxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxs ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bxs OCA], [https://pdbe.org/4bxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bxs RCSB], [https://www.ebi.ac.uk/pdbsum/4bxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bxs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/FA5V_PSETE FA5V_PSETE]] Snake prothrombin activator that attacks the hemostatic system of prey. This non-catalytic subunit is functionally similar to blood coagulation factor V. It serves as a critical cofactor for the prothrombinase activity of the catalytic subunit, which is similar to the blood coagulation factor X.<ref>PMID:12362232</ref>
[https://www.uniprot.org/uniprot/FAXC_PSETE FAXC_PSETE] Snake prothrombin activator that attacks the hemostatic system of prey. This non-catalytic subunit is functionally similar to blood coagulation factor V (PubMed:12362232, PubMed:23869089). It serves as a critical cofactor for the prothrombinase activity of the catalytic subunit, which is similar to the blood coagulation factor X (PubMed:12362232, PubMed:23869089). The complex converts prothrombin to thrombin by sequential cleavage at two positions, Arg-320 followed by Arg-271 (PubMed:23869089). Cleavage at Arg-320 produces an active intermediate known as meizothrombin (PubMed:23869089). Meizothrombin is the 'second' substrate for prothrombinase, and it docks in an altered manner to present the second cleavage site (271) (PubMed:23869089). Cleavage at Arg-271 releases active thrombin from its pro-fragment (PubMed:23869089). This order of events is reversed if the protease component of prothrombinase is used on its own, suggesting that the 271 site is inherently more accessible to proteolysis (PubMed:23869089). The complex converts prothrombin to thrombin in presence but also in the absence of membrane (PubMed:23869089).<ref>PMID:12362232</ref> <ref>PMID:23869089</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Eastern brown snake]]
[[Category: Large Structures]]
[[Category: Adams, T E]]
[[Category: Pseudonaja textilis]]
[[Category: Camire, R M]]
[[Category: Adams TE]]
[[Category: Huntington, J A]]
[[Category: Camire RM]]
[[Category: Johnson, D J.D]]
[[Category: Huntington JA]]
[[Category: Krishnaswamy, S]]
[[Category: Johnson DJD]]
[[Category: Lechtenberg, B C]]
[[Category: Krishnaswamy S]]
[[Category: Murray-Rust, T A]]
[[Category: Lechtenberg BC]]
[[Category: Blood clotting]]
[[Category: Murray-Rust TA]]
[[Category: Blood coagulation]]
[[Category: Hydrolase]]
[[Category: Prothrombinase]]
Retrieved from "https://proteopedia.org/4bxs"