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[[Image:2jt7.jpg|left|200px]]


{{Structure
==NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex==
|PDB= 2jt7 |SIZE=350|CAPTION= <scene name='initialview01'>2jt7</scene>
<StructureSection load='2jt7' size='340' side='right'caption='[[2jt7]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DMY:DISTAMYCIN A'>DMY</scene>
<table><tr><td colspan='2'>[[2jt7]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JT7 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jt7 OCA], [https://pdbe.org/2jt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2jt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jt7 ProSAT]</span></td></tr>
 
</table>
'''NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex'''
<div style="background-color:#fffaf0;">
 
== Publication Abstract from PubMed ==
 
==Overview==
The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure.
The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure.


==About this Structure==
Structural and thermodynamic studies of the interaction of distamycin A with the parallel quadruplex structure [d(TGGGGT)]4.,Martino L, Virno A, Pagano B, Virgilio A, Di Micco S, Galeone A, Giancola C, Bifulco G, Mayol L, Randazzo A J Am Chem Soc. 2007 Dec 26;129(51):16048-56. Epub 2007 Dec 4. PMID:18052170<ref>PMID:18052170</ref>
2JT7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JT7 OCA].
 
==Reference==
Structural and thermodynamic studies of the interaction of distamycin A with the parallel quadruplex structure [d(TGGGGT)]4., Martino L, Virno A, Pagano B, Virgilio A, Di Micco S, Galeone A, Giancola C, Bifulco G, Mayol L, Randazzo A, J Am Chem Soc. 2007 Dec 26;129(51):16048-56. Epub 2007 Dec 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18052170 18052170]
[[Category: Single protein]]
[[Category: Martino, L.]]
[[Category: Virno, A.]]
[[Category: DMY]]
[[Category: complex]]
[[Category: distamycin some]]
[[Category: dna]]
[[Category: quadruplex]]
[[Category: telomere]]
[[Category: tggggt]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:44:58 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2jt7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Martino L]]
[[Category: Virno A]]
Retrieved from "https://proteopedia.org/2jt7"