2opc: Difference between revisions
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< | ==Structure of Melampsora lini avirulence protein, AvrL567-A== | ||
<StructureSection load='2opc' size='340' side='right'caption='[[2opc]], [[Resolution|resolution]] 1.43Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2opc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Melampsora_lini Melampsora lini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OPC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43Å</td></tr> | |||
- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2opc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opc OCA], [https://pdbe.org/2opc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2opc RCSB], [https://www.ebi.ac.uk/pdbsum/2opc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2opc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6R661_MELLI Q6R661_MELLI] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/2opc_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2opc ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available. | |||
The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein.,Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816<ref>PMID:17329816</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2opc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
== | |||
< | |||
[[Category: Melampsora lini]] | [[Category: Melampsora lini]] | ||
[[Category: Catanzariti | [[Category: Catanzariti AM]] | ||
[[Category: Dodds | [[Category: Dodds PN]] | ||
[[Category: Ellis | [[Category: Ellis JG]] | ||
[[Category: Forwood | [[Category: Forwood JK]] | ||
[[Category: Guncar | [[Category: Guncar G]] | ||
[[Category: Kobe | [[Category: Kobe B]] | ||
[[Category: Teh | [[Category: Teh T]] | ||
[[Category: Wang | [[Category: Wang CI]] | ||
Latest revision as of 00:21, 28 December 2023
Structure of Melampsora lini avirulence protein, AvrL567-A
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