4tql: Difference between revisions
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==Computationally designed three helix bundle== | ==Computationally designed three helix bundle== | ||
<StructureSection load='4tql' size='340' side='right' caption='[[4tql]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='4tql' size='340' side='right'caption='[[4tql]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4tql]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQL OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4tql]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TQL FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tql OCA], [https://pdbe.org/4tql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tql RCSB], [https://www.ebi.ac.uk/pdbsum/4tql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tql ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe a procedure for designing proteins with backbones produced by varying the parameters in the Crick coiled coil-generating equations. Combinatorial design calculations identify low-energy sequences for alternative helix supercoil arrangements, and the helices in the lowest-energy arrangements are connected by loop building. We design an antiparallel monomeric untwisted three-helix bundle with 80-residue helices, an antiparallel monomeric right-handed four-helix bundle, and a pentameric parallel left-handed five-helix bundle. The designed proteins are extremely stable (extrapolated DeltaGfold > 60 kilocalories per mole), and their crystal structures are close to those of the design models with nearly identical core packing between the helices. The approach enables the custom design of hyperstable proteins with fine-tuned geometries for a wide range of applications. | |||
High thermodynamic stability of parametrically designed helical bundles.,Huang PS, Oberdorfer G, Xu C, Pei XY, Nannenga BL, Rogers JM, DiMaio F, Gonen T, Luisi B, Baker D Science. 2014 Oct 24;346(6208):481-5. doi: 10.1126/science.1257481. PMID:25342806<ref>PMID:25342806</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4tql" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: | [[Category: Baker D]] | ||
[[Category: | [[Category: DiMaio F]] | ||
[[Category: | [[Category: Gonen T]] | ||
[[Category: | [[Category: Nannenga BL]] | ||
[[Category: | [[Category: Oberdorfer G]] | ||
