4wvm: Difference between revisions

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~~'''Unreleased structure'''~~

The ~~entry 4wvm~~ is ~~ON HOLD~~

==Stonustoxin structure==

== Structural highlights ==

<table><tr><td colspan='2'>[[4wvm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synanceia_horrida Synanceia horrida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WVM FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wvm OCA], [https://pdbe.org/4wvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wvm RCSB], [https://www.ebi.ac.uk/pdbsum/4wvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wvm ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/STXA_SYNHO STXA_SYNHO] This lethal (towards mice) toxin induces hemolytic activities due to its ability to form pores in the cell membrane, elicits potent hypotension which is endothelium-dependent and appears to be mediated by the nitric oxide pathway and activation of potassium channels, displays edema-inducing activities, increases vascular permeability, induces endothelium-dependent vasorelaxation of isolated rat aorta, induces platelet aggregation, is myotoxic and interferes irreversibly with neuromuscular function.<ref>PMID:1790672</ref> <ref>PMID:8079369</ref> <ref>PMID:9271089</ref>

== Publication Abstract from PubMed ==

The lethal factor in stonefish venom is stonustoxin (SNTX), a heterodimeric cytolytic protein that induces cardiovascular collapse in humans and native predators. Here, using X-ray crystallography, we make the unexpected finding that SNTX is a pore-forming member of an ancient branch of the Membrane Attack Complex-Perforin/Cholesterol-Dependent Cytolysin (MACPF/CDC) superfamily. SNTX comprises two homologous subunits (alpha and beta), each of which comprises an N-terminal pore-forming MACPF/CDC domain, a central focal adhesion-targeting domain, a thioredoxin domain, and a C-terminal tripartite motif family-like PRY SPla and the RYanodine Receptor immune recognition domain. Crucially, the structure reveals that the two MACPF domains are in complex with one another and arranged into a stable early prepore-like assembly. These data provide long sought after near-atomic resolution insights into how MACPF/CDC proteins assemble into prepores on the surface of membranes. Furthermore, our analyses reveal that SNTX-like MACPF/CDCs are distributed throughout eukaryotic life and play a broader, possibly immune-related function outside venom.

~~Authors:~~ Ellisdon, ~~A.M.~~, Panjikar, S., ~~Whisstock~~, J~~.C.~~, McGowan, S.

Stonefish toxin defines an ancient branch of the perforin-like superfamily.,Ellisdon AM, Reboul CF, Panjikar S, Huynh K, Oellig CA, Winter KL, Dunstone MA, Hodgson WC, Seymour J, Dearden PK, Tweten RK, Whisstock JC, McGowan S Proc Natl Acad Sci U S A. 2015 Dec 1. pii: 201507622. PMID:26627714<ref>PMID:26627714</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

==See Also==

*[[Cytolysin 3D structures|Cytolysin 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Synanceia horrida]]

[[Category: Ellisdon AM]]

[[Category: McGowan S]]

[[Category: Panjikar S]]

[[Category: Whisstock JC]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4wvm is ON HOLD
+==Stonustoxin structure==
+<StructureSection load='4wvm' size='340' side='right'caption='[[4wvm]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4wvm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synanceia_horrida Synanceia horrida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WVM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wvm OCA], [https://pdbe.org/4wvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wvm RCSB], [https://www.ebi.ac.uk/pdbsum/4wvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wvm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/STXA_SYNHO STXA_SYNHO] This lethal (towards mice) toxin induces hemolytic activities due to its ability to form pores in the cell membrane, elicits potent hypotension which is endothelium-dependent and appears to be mediated by the nitric oxide pathway and activation of potassium channels, displays edema-inducing activities, increases vascular permeability, induces endothelium-dependent vasorelaxation of isolated rat aorta, induces platelet aggregation, is myotoxic and interferes irreversibly with neuromuscular function.<ref>PMID:1790672</ref> <ref>PMID:8079369</ref> <ref>PMID:9271089</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The lethal factor in stonefish venom is stonustoxin (SNTX), a heterodimeric cytolytic protein that induces cardiovascular collapse in humans and native predators. Here, using X-ray crystallography, we make the unexpected finding that SNTX is a pore-forming member of an ancient branch of the Membrane Attack Complex-Perforin/Cholesterol-Dependent Cytolysin (MACPF/CDC) superfamily. SNTX comprises two homologous subunits (alpha and beta), each of which comprises an N-terminal pore-forming MACPF/CDC domain, a central focal adhesion-targeting domain, a thioredoxin domain, and a C-terminal tripartite motif family-like PRY SPla and the RYanodine Receptor immune recognition domain. Crucially, the structure reveals that the two MACPF domains are in complex with one another and arranged into a stable early prepore-like assembly. These data provide long sought after near-atomic resolution insights into how MACPF/CDC proteins assemble into prepores on the surface of membranes. Furthermore, our analyses reveal that SNTX-like MACPF/CDCs are distributed throughout eukaryotic life and play a broader, possibly immune-related function outside venom.
-Authors: Ellisdon, A.M., Panjikar, S., Whisstock, J.C., McGowan, S.
+Stonefish toxin defines an ancient branch of the perforin-like superfamily.,Ellisdon AM, Reboul CF, Panjikar S, Huynh K, Oellig CA, Winter KL, Dunstone MA, Hodgson WC, Seymour J, Dearden PK, Tweten RK, Whisstock JC, McGowan S Proc Natl Acad Sci U S A. 2015 Dec 1. pii: 201507622. PMID:26627714<ref>PMID:26627714</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4wvm" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Cytolysin 3D structures|Cytolysin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Synanceia horrida]]
+[[Category: Ellisdon AM]]
+[[Category: McGowan S]]
+[[Category: Panjikar S]]
+[[Category: Whisstock JC]]